LOCUS       sequence-424543-       10619 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pegRNA/ngRNA library cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10619)
  AUTHORS   Ren X, Yang H, Nierenberg JL, Sun Y, Chen J, Beaman C, Pham T, 
            Nobuhara M, Takagi MA, Narayan V, Li Y, Ziv E, Shen Y
  TITLE     High-throughput PRIME-editing screens identify functional DNA 
            variants in the human genome.
  JOURNAL   Mol Cell. 2023 Dec 21;83(24):4633-4645.e9. doi: 
            10.1016/j.molcel.2023.11.021.
  PUBMED    38134886
REFERENCE   2  (bases 1 to 10619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.11"; journalName: "Mol Cell"; date: 
            "2023-12-21- 21"; volume: "83"; issue: "24"; pages: "4633-4645.e9"
COMMENT     Sequence Label: LentiV2-mU6-evopreQ1
FEATURES             Location/Qualifiers
     source          1..10619
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..202
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     157..177
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             220..400
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    447..572
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1065..1298
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1483..1527
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1825..1942
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1993..2306
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     2225..2245
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        3026..3237
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             3257..4276
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     CDS             4295..4348
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      4355..4364
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4361..4363,4364..4366,4367..5080)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4361..4363,4364..4366,4367..5077)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4406..4427)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4667..4686)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5014..5035
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5096..5684
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5149..5169)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5567..5578)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5756..5989
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6015..6032)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6021..6245
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6291..6719
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6378..6397)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6588..6609
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6728..6748)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6733..7062
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6913..7048
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6975..6994
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7110..7157
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7176..7550
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7680..7801
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7717..7736)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7771..7790
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7850..7866)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7850..7866)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7863..7885)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7874..7890
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7898..7904,7905..7922,7923..7928))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7943..7964
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8081..8098)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8252..8840)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8332..8351)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9011..9802,9803..9871))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9634..9653
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9872..9976)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10051..10070)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(10242..10619,1..2)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
       61 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      121 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
      181 ggtgggaggt ctatataagc agcgcgtttt gcctgtactg ggtctctctg gttagaccag
      241 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
      301 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
      361 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
      421 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
      481 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
      541 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
      601 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
      661 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
      721 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
      781 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
      841 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
      901 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
      961 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     1021 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     1081 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     1141 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     1201 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     1261 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     1321 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     1381 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     1441 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     1501 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     1561 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     1621 gtactttcta tagtgaatat ccctgcctaa ctctattcac cattatcgtt tcagacccac
     1681 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     1741 gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc aattctgcag
     1801 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     1861 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     1921 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggtt
     1981 aattaaggta ccgatccgac gccgccatct ctaggcccgc gccggccccc tcgcacagac
     2041 ttgtgggaga agctcggcta ctcccctgcc ccggttaatt tgcatataat atttcctagt
     2101 aactatagag gcttaatgtg cgataaaaga cagataatct gttcttttta atactagcta
     2161 cattttacat gataggcttg gatttctata agagatacaa atactaaatt attattttaa
     2221 aaaacagcac aaaaggaaac tcaccctaac tgtaaagtaa ttgtgtgttt tgagactata
     2281 aatatccctt ggagaaaagc cttgtttgga gacggttgta aatgagcaca caaaatacac
     2341 atgctaaaat attatattct atgaccttta taaaatcaac caaaatcttc tttttaataa
     2401 ctttagtatc aataattaga atttttatgt tcctttttgc aaacttttaa taaaaatgag
     2461 caaaataaaa aaacgctagt tttagtaact cgcgttgttt tcttcacctt taataatagc
     2521 tactccacca cttgttccta agcggtcagc tcctgcttca atcatttttt gagcatcttc
     2581 aaatgttcta actccaccag ctgctttaac taaagcattg tctttaacaa ctgacttcat
     2641 tagtttaaca tcttcaaatg ttgcacctga ttttgaaaat cctgttgatg ttttaacaaa
     2701 ttctaatcca gcttcaacag ctatttcaca agctttcatg atttcttctt ttgttaataa
     2761 acaattttcc ataatacatt taacaacatg tgatccagct gcttttttta cagctttcat
     2821 gtcttctaaa actaattcat aatttttgtc ttttaatgca ccaatattta ataccatatc
     2881 aatttctgtt gcaccatctt taattgctac gtctctccta acaccgcggt tctatctagt
     2941 tacgcgttaa accaactaga aatttttttt gaattcgcta gctaggtctt gaaaggagtg
     3001 ggaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     3061 ttggggggag gggtcggcaa ttgatccggt gcctagagaa ggtggcgcgg ggtaaactgg
     3121 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     3181 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggac
     3241 cggttctaga gccaccatga aaaagcctga actcaccgct acctctgtcg agaagtttct
     3301 gatcgaaaag ttcgacagcg tgtccgacct gatgcagctc tccgagggcg aagaatctcg
     3361 ggctttcagc ttcgatgtgg gagggcgtgg atatgtcctg cgggtgaata gctgcgccga
     3421 tggtttctac aaagatcgct atgtttatcg gcactttgca tccgccgctc tccctattcc
     3481 cgaagtgctt gacattgggg agttcagcga gagcctgacc tattgcatct cccgccgtgc
     3541 acagggtgtc accttgcaag acctgcctga aaccgaactg cccgctgttc tccagcccgt
     3601 cgccgaggcc atggatgcca tcgctgccgc cgatcttagc cagaccagcg ggttcggccc
     3661 attcggacct caaggaatcg gtcaatacac tacatggcgc gatttcatct gcgctattgc
     3721 tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg cctccgtcgc
     3781 ccaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt
     3841 gcacgccgat ttcggctcca acaatgtcct gaccgacaat ggccgcataa cagccgtcat
     3901 tgactggagc gaggccatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg
     3961 gaggccctgg ttggcttgta tggagcagca gacccgctac ttcgagcgga ggcatcccga
     4021 gcttgcagga tctcctcggc tccgggctta tatgctccgc attggtcttg accaactcta
     4081 tcagagcttg gttgacggca atttcgatga tgcagcttgg gctcagggtc gctgcgacgc
     4141 aatcgtccgg tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgctgc
     4201 cgtctggacc gatggctgtg tggaagtgct cgccgatagt ggaaacagac gccccagcac
     4261 tcgtcctagg gcaaaggatc tgcagggcag tggagagggc agaggaagtc tgctaacatg
     4321 cggtgacgtc gaggagaatc ctggcccaag cgctgccacc atggtgagca agggcgagga
     4381 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     4441 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     4501 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     4561 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     4621 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     4681 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     4741 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     4801 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     4861 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     4921 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     4981 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     5041 cgccgggatc actctcggca tggacgagct gtacaagtaa acgcgttaag tcgacaatca
     5101 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     5161 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     5221 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     5281 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     5341 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     5401 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     5461 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     5521 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     5581 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     5641 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca
     5701 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     5761 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     5821 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     5881 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     5941 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggcccgttta
     6001 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     6061 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     6121 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     6181 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     6241 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     6301 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     6361 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     6421 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     6481 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     6541 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     6601 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     6661 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     6721 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     6781 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     6841 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     6901 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     6961 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     7021 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagtttgt
     7081 atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg
     7141 gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc
     7201 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt
     7261 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt
     7321 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc
     7381 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg
     7441 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc
     7501 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac
     7561 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     7621 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     7681 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     7741 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     7801 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     7861 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     7921 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     7981 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     8041 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     8101 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     8161 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     8221 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     8281 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     8341 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     8401 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     8461 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     8521 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     8581 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     8641 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     8701 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     8761 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     8821 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     8881 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     8941 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     9001 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     9061 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     9121 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     9181 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     9241 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     9301 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     9361 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     9421 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     9481 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     9541 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     9601 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     9661 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     9721 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     9781 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     9841 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     9901 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     9961 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
    10021 atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag
    10081 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
    10141 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
    10201 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
    10261 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
    10321 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
    10381 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
    10441 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
    10501 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
    10561 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattacca
//
