LOCUS       sequence-424545-       10549 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pegRNA/ngRNA library cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10549)
  AUTHORS   Ren X, Yang H, Nierenberg JL, Sun Y, Chen J, Beaman C, Pham T, 
            Nobuhara M, Takagi MA, Narayan V, Li Y, Ziv E, Shen Y
  TITLE     High-throughput PRIME-editing screens identify functional DNA 
            variants in the human genome.
  JOURNAL   Mol Cell. 2023 Dec 21;83(24):4633-4645.e9. doi: 
            10.1016/j.molcel.2023.11.021.
  PUBMED    38134886
REFERENCE   2  (bases 1 to 10549)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.11"; journalName: "Mol Cell"; date: 
            "2023-12-21- 21"; volume: "83"; issue: "24"; pages: "4633-4645.e9"
COMMENT     Sequence Label: LentiV2-hU6-evopreQ1
FEATURES             Location/Qualifiers
     source          1..10549
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..219
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     174..194
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             237..417
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    464..589
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1082..1315
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1500..1544
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1842..1959
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2010..2258
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2010..2030
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2181..2200
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        2973..3184
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             3204..4223
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     CDS             4242..4295
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      4302..4311
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4308..4310,4311..4313,4314..5027)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4308..4310,4311..4313,4314..5024)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4353..4374)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4614..4633)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4961..4982
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5043..5631
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5096..5116)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5514..5525)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5703..5936
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5962..5979)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5968..6192
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6238..6666
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6325..6344)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6535..6556
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6675..6695)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6680..7009
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6860..6995
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6922..6941
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7057..7104
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7123..7497
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7627..7748
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7664..7683)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7718..7737
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7797..7813)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7797..7813)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7810..7832)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7821..7837
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7845..7851,7852..7869,7870..7875))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7890..7911
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8028..8045)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8199..8787)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8279..8298)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8958..9749,9750..9818))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9581..9600
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9819..9923)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9998..10017)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(10189..10549,1..19)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
       61 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      121 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      181 gggcggtagg cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt
      241 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
      301 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
      361 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
      421 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
      481 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
      541 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
      601 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
      661 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
      721 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
      781 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
      841 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
      901 caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata
      961 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     1021 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     1081 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     1141 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     1201 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     1261 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     1321 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     1381 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     1441 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     1501 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     1561 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     1621 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     1681 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
     1741 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tcggcactgc
     1801 gtgcgccaat tctgcagaca aatggcagta ttcatccaca attttaaaag aaaagggggg
     1861 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
     1921 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
     1981 agagatccag tttggttaat taaggtaccg agggcctatt tcccatgatt ccttcatatt
     2041 tgcatatacg atacaaggct gttagagaga taattagaat taatttgact gtaaacacaa
     2101 agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt
     2161 taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt
     2221 tcttggcttt atatatcttg tggaaaggac gaaacaccgg agacggttgt aaatgagcac
     2281 acaaaataca catgctaaaa tattatattc tatgaccttt ataaaatcaa ccaaaatctt
     2341 ctttttaata actttagtat caataattag aatttttatg ttcctttttg caaactttta
     2401 ataaaaatga gcaaaataaa aaaacgctag ttttagtaac tcgcgttgtt ttcttcacct
     2461 ttaataatag ctactccacc acttgttcct aagcggtcag ctcctgcttc aatcattttt
     2521 tgagcatctt caaatgttct aactccacca gctgctttaa ctaaagcatt gtctttaaca
     2581 actgacttca ttagtttaac atcttcaaat gttgcacctg attttgaaaa tcctgttgat
     2641 gttttaacaa attctaatcc agcttcaaca gctatttcac aagctttcat gatttcttct
     2701 tttgttaata aacaattttc cataatacat ttaacaacat gtgatccagc tgcttttttt
     2761 acagctttca tgtcttctaa aactaattca taatttttgt cttttaatgc accaatattt
     2821 aataccatat caatttctgt tgcaccatct ttaattgcta cgtctctttg acgcggttct
     2881 atctagttac gcgttaaacc aactagaaat tttttttgaa ttcgctagct aggtcttgaa
     2941 aggagtggga attggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     3001 cgagaagttg gggggagggg tcggcaattg atccggtgcc tagagaaggt ggcgcggggt
     3061 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     3121 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     3181 acaggaccgg ttctagagcc accatgaaaa agcctgaact caccgctacc tctgtcgaga
     3241 agtttctgat cgaaaagttc gacagcgtgt ccgacctgat gcagctctcc gagggcgaag
     3301 aatctcgggc tttcagcttc gatgtgggag ggcgtggata tgtcctgcgg gtgaatagct
     3361 gcgccgatgg tttctacaaa gatcgctatg tttatcggca ctttgcatcc gccgctctcc
     3421 ctattcccga agtgcttgac attggggagt tcagcgagag cctgacctat tgcatctccc
     3481 gccgtgcaca gggtgtcacc ttgcaagacc tgcctgaaac cgaactgccc gctgttctcc
     3541 agcccgtcgc cgaggccatg gatgccatcg ctgccgccga tcttagccag accagcgggt
     3601 tcggcccatt cggacctcaa ggaatcggtc aatacactac atggcgcgat ttcatctgcg
     3661 ctattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcct
     3721 ccgtcgccca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc
     3781 acctcgtgca cgccgatttc ggctccaaca atgtcctgac cgacaatggc cgcataacag
     3841 ccgtcattga ctggagcgag gccatgttcg gggattccca atacgaggtc gccaacatct
     3901 tcttctggag gccctggttg gcttgtatgg agcagcagac ccgctacttc gagcggaggc
     3961 atcccgagct tgcaggatct cctcggctcc gggcttatat gctccgcatt ggtcttgacc
     4021 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggct cagggtcgct
     4081 gcgacgcaat cgtccggtcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa
     4141 gcgctgccgt ctggaccgat ggctgtgtgg aagtgctcgc cgatagtgga aacagacgcc
     4201 ccagcactcg tcctagggca aaggatctgc agggcagtgg agagggcaga ggaagtctgc
     4261 taacatgcgg tgacgtcgag gagaatcctg gcccaagcgc tgccaccatg gtgagcaagg
     4321 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     4381 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     4441 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     4501 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     4561 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     4621 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     4681 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     4741 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     4801 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     4861 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     4921 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     4981 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaacg cgttaagtcg
     5041 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     5101 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     5161 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     5221 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     5281 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     5341 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     5401 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     5461 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     5521 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     5581 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cgtcgacttt
     5641 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     5701 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     5761 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     5821 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     5881 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc
     5941 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     6001 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     6061 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     6121 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     6181 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     6241 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     6301 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     6361 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     6421 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     6481 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     6541 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     6601 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     6661 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     6721 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     6781 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     6841 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     6901 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     6961 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     7021 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
     7081 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt
     7141 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
     7201 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
     7261 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
     7321 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
     7381 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
     7441 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac
     7501 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     7561 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     7621 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     7681 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     7741 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     7801 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     7861 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     7921 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     7981 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     8041 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     8101 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     8161 aaggccagga accgtaaaaa gggcgcgttg ctggcgtttt tccataggct ccgcccccct
     8221 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     8281 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     8341 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     8401 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     8461 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     8521 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     8581 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     8641 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     8701 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     8761 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     8821 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     8881 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     8941 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     9001 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     9061 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     9121 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     9181 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     9241 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     9301 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     9361 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     9421 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     9481 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     9541 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     9601 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     9661 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     9721 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     9781 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     9841 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     9901 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
     9961 tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat
    10021 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
    10081 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
    10141 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat
    10201 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
    10261 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
    10321 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
    10381 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
    10441 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
    10501 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatt
//
