LOCUS       sequence-42457-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  TITLE     Peter Murray lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3.1-mArg1-Flag
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..59
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        40..58
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             142..165
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ggctactaga gaacccactg cttactggct tatcgaaatt aatacgactc actataggga
       61 gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc cactagtcca
      121 gtgtggtgga attccaccat ggactacaag gacgacgatg acaagagctc caagccaaag
      181 tccttagaga ttatcggagc gcctttctca aaaggacagc ctcgaggagg ggtagagaaa
      241 ggccctgcag cactgaggaa agctggtctg ctggaaaaac ttaaagaaac agagtatgac
      301 gtgagagacc acggggacct ggcctttgtt gatgtcccta atgacagctc ctttcaaatt
      361 gtgaagaacc cacggtctgt ggggaaagcc aatgaagagc tggctggtgt ggtggcagag
      421 gtccagaaga atggaagagt cagtgtggtg ctgggtggag accacagtct ggcagttgga
      481 agcatctctg gccacgccag ggtccaccct gacctatgtg tcatttgggt ggatgctcac
      541 actgacatca acactcccct gacaaccagc tctgggaatc tgcatgggca acctgtgtcc
      601 tttctcctga aggaactgaa aggaaagttc ccagatgtac caggattctc ctgggtgact
      661 ccctgcatat ctgccaaaga catcgtgtac attggcttgc gagacgtaga ccctggggaa
      721 cactatataa taaaaactct gggaattaag tatttctcca tgactgaagt agacaagctg
      781 gggattggca aggtgatgga agagaccttc agctacctgc tgggaaggaa gaaaaggccg
      841 attcacctga gctttgatgt cgatgggctg gaccca
//
