LOCUS       sequence-424576-        7344 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human LLGL2. N-terminal HiBiT 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7344)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 7344)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-N_LLGL2:1-1020
FEATURES             Location/Qualifiers
     source          1..7344
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    2046..2167
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2057..2076)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(2282..2315)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2325..2372
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2404..3198
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2458..2477)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3068..3087
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(3356..3944)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3436..3455)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4070..4118
                     /note="synthetic polyadenylation signal"
     CDS             complement(4142..4540)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      4537..4546
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4571..4928)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4585..4720
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        4737..4928
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     4885..4905
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    5007..5088
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        5321..5624
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5625..5828
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5779..5799
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          5964..6096
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     6079..6098
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     6140..6159
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6140..6158
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gccaccggtc cagctgccct acctggcttc tctgcactgt tccgccatca cctgctctca
       61 ccacgtctcc aacatcccgc tgaagctgtg ggagcggatc attgccgccg gcagccggca
      121 gaacgcacac ttctccacca tggagtggcc aattgatggt ggcaccagcc tgaccccagc
      181 cccaccccag agggacctgc tgctcacagg gcacgaggac ggcacggtgc ggttctggga
      241 tgcctcgggt gtctgcctgc ggctgctcta caaactcagc actgtgcgcg tgttcctcac
      301 cgacacggac cccaacgaga acctcagtgc ccagggcgag gacgagtggc ccccactccg
      361 caaggtgggc tcctttgacc cctacagtga tgacccccgg ctgggcatcc agaagatctt
      421 cctctgcaag tacagcggct acctggctgt ggcaggcacg gcagggcagg tgctggtact
      481 ggaactgaat gacgaggcag cggagcaggc tgtggagcag gtggaggccg acctgctgca
      541 ggaccaagag ggctaccgct ggaaggggca cgagcgcctg gcagcccgct cagggcccgt
      601 gcgctttgag cctggctttc agcccttcgt gttggtgcag tgtcagcccc cggctgtggt
      661 cacctccttg gccctgcact ctgagtggcg gctcgtggcc ttcggcacca gccatggctt
      721 tggcctcttt gaccaccagc agcggcggca ggtctttgtt aagtgcacac tgcaccccag
      781 tgaccagctg gccttggagg gcccactctc ccgcgtcaag tccctcaaga agtccttgcg
      841 tcagtcattc cgccggatgc gtcggagccg ggtgtccagc cggaagcggc acccagctgg
      901 ccccccagga gaggcacagg aggggagtgc caaggctgag cggccaggcc tccagaacat
      961 ggagctggcg cctgtgcagc gcaagatcga ggctcgctcg gcagaggact ccttcacagg
     1021 cttcgtccgg accctgtact ttgctgacac ctacctgaag gacagctccc ggcactgccc
     1081 ctcgctgtgg gctggcacca atgggggcac catctatgcc ttctccctgc gtgtgcctcc
     1141 cgccgagcgg agaatggatg agtctgtgcg ggcagagcag gccaaggaga tccagctgat
     1201 gcaccgggcg ccggtggtgg gcatcctggt gctcgacgga cacagcgtac cccttcctga
     1261 gcccctcgaa gtggcccatg atctgtcgaa gagccctgac atgcagggaa gccaccagct
     1321 gctcgtcgta tcagaggagc agttcaaggt gttcacgctg cccaaggtga gtgccaagct
     1381 gaagttgaag ctgacggccc tggagggctc aagagtgcgg cgggtcagcg tggcccactt
     1441 cggcagtcgt cgagccgagg actacgggga gcaccacctg gcagtcctta ccaacctggg
     1501 cgacatccag gtggtctcac tgcccctgct caagccccag gtgcgctaca gctgcatccg
     1561 ccgggaggac gtcagtggca tcgcctcctg cgtcttcacc aaatatggcc aaggcttcta
     1621 cctgatctca ccctcggagt ttgagcgctt ctctctctcc accaagtggc tggtggagcc
     1681 ccggtgtctg gtggattcag cagaaaccaa gaaccaccgc cctggtaacg gtgcgggccc
     1741 caagaaggcc ccgagccgag ccaggaactc agggactcag agtgatggcg aggagaagca
     1801 gcccggcctg gtgatggagc gcgctctgct cagtgatgag agagtcctga aggaaatcca
     1861 gagcacactg gagggagacc gcgggagcgg caactggcgt tcacatcgag cggccgtggg
     1921 gtgcagcctc agcaatggcg gagcagagta atctagaggt caagcttcgg gggtttaaac
     1981 ctgcaggcat gcaagctgat ttggctgctg ccaccgctga gcaaggccgc ttcgagcaga
     2041 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     2101 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     2161 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg agatgtggga
     2221 ggttttttta agcaagtaaa acctctacaa atgtggtaaa atcttaatta agacttctaa
     2281 tataacttcg tatagcatac attatacgaa gttatggttc cactgttgac aattaatcat
     2341 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc caggaggcag
     2401 atcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     2461 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     2521 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     2581 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     2641 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     2701 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     2761 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     2821 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     2881 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     2941 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     3001 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     3061 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     3121 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     3181 cttgacgagt tcttctgatt cgaaatgacc gaccaagcga cgcccaaccg gtatcagctc
     3241 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     3301 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     3361 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     3421 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     3481 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     3541 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     3601 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     3661 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     3721 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     3781 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     3841 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     3901 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatt tcaagaagat cctttgatct
     3961 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     4021 gattatcaaa aaggatcttc acctagatcc ttttatagtc cggaatcgtc acacaaaaaa
     4081 ccaacacaca gatgtaatga aaataaagat attttattgc ggccatcgtg atggctagag
     4141 tttagccctc ccacacataa ccagagggca gcaattcacg aatcccaact gccgtcggct
     4201 gtccatcact gtccttcact atggctttga tcccaggatg cagatcgaga agcacctgtc
     4261 ggcaccgtcc gcaggggctc aagatgcccc tgttctcatt tccgatcgcg acgatacaag
     4321 tcaggttgcc agctgccgca gcagcagcag tgcccagcac cacgagttct gcacaaggtc
     4381 ccccagtaaa atgatataca ttgacaccag tgaagatgcg gccgtcgcta gagagagctg
     4441 cgctggcgac gctgtagtct tcagagatgg ggatgctgtt gattgtagcc gttgctcttt
     4501 caatgagggt ggattcttct tgagacaaag gcttggccat ggtggcgcta gtgtcagaag
     4561 aatcgagctt tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata
     4621 gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg
     4681 gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga
     4741 ctatggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg
     4801 gggactttcc acacctggtt gctgactaat tgagatgcat gctttgcata cttctgcctg
     4861 ctggggagcc tggggacttt ccacacccta actgacacac attccacagc tggttctttc
     4921 cgcctcagaa ggtacctaac caagttcctc tttcagaggt tatttcaggc catggtgctg
     4981 cgcaagacgc gttaattaag acgtcggcag tgaaaaaaat gctttatttg tgaaatttgt
     5041 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     5101 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     5161 aacctctaca aatgtggtat ggctgattat gatccgcggc cgcaatacgt cgacgttatc
     5221 agctgacttc gtacgagagc ctaggattat ggcgcgccac tagttattaa tagtaatcaa
     5281 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     5341 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     5401 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     5461 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtccgccc cctattgacg
     5521 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc
     5581 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     5641 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     5701 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     5761 ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     5821 gcagagctgg tttagtgaac cgtcagatca ctagaatctt tattgcggta gtttatcaca
     5881 gttaaattgc taacgcagtc agtgcttctg acacaacagt ctcgaactta agctgcagaa
     5941 gttggtcgtg aggcactggg caggtaagta tcaaggttac aagacaggtt taaggagacc
     6001 aatagaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag gcacctattg
     6061 gtcttactga catccacttt gcctttctct ccacaggtgt ccactcccag ttcaattaca
     6121 gctcttaagg ctagagtatt aatacgactc actatagggg ttagcgatcg ccatggtgag
     6181 cggctggcgg ctgttcaaga agattagcgg gagctccggt ggctcgagcg gtatgaggcg
     6241 gttcctgagg ccagggcatg accctgtgcg ggagaggctc aagcgggacc tgttccagtt
     6301 taacaagacg gtggagcatg gcttcccgca ccagcccagc gccctcggct acagcccgtc
     6361 cctgcgcatc ctggccatcg gcacccgttc tggagccatc aagctctacg gagccccagg
     6421 cgtggagttc atggggctgc accaggagaa caacgctgtg acgcagatcc acctcctgcc
     6481 cggccagtgc cagctggtca ccctgctgga tgacaacagc ctgcaccttt ggagcctgaa
     6541 ggtcaagggc ggggcatcgg agctgcagga ggatgagagc ttcacactgc gtggaccccc
     6601 aggggctgcc cccagtgcca cacagatcac cgtggtcctg ccacattcct cctgcgagct
     6661 gctctacctg ggcaccgaga gtggcaacgt gtttgtggtg cagctgccag cttttcgtgc
     6721 gctggaggac cggaccatca gctcggacgc ggtgctgcag cggttgccag aggaggcccg
     6781 ccaccggcgt gtgttcgaga tggtggaggc actgcaggag caccctcgag accccaacca
     6841 gatcctgatc ggctacagcc gaggcctcgt tgtcatctgg gacctacagg gcagccgcgt
     6901 gctctaccac ttcctcagca gccagcaact ggagaacatc tggtggcagc gggacggccg
     6961 cctgctcgtc agctgtcact ctgacggcag ctactgccag tggcccgtgt ccagcgaagc
     7021 ccagcaacca gagcccctcc gcagcctcgt gccttacggt ccctttcctt gcaaagcgat
     7081 taccagaatc ctctggctga ccactaggca ggggttgccc ttcaccatct tccagggtgg
     7141 catgccacgg gccagctacg gggaccgcca ctgcatctca gtgatccacg atggccagca
     7201 gacggccttc gacttcacct cccgtgtcat cggcttcact gtcctcacag aggcagaccc
     7261 tgcagccacc tttgacgacc cctatgccct ggtggtgctg gctgaggagg agctggtggt
     7321 gattgacctg cagacagcag gctg
//
