LOCUS       sequence-424636-        6577 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient expression of STING.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6577)
  AUTHORS   Dobbs N, Burnaevskiy N, Chen D, Gonugunta VK, Alto NM, Yan N
  TITLE     STING Activation by Translocation from the ER Is Associated with 
            Infection and Autoinflammatory Disease.
  JOURNAL   Cell Host Microbe. 2015 Aug 12;18(2):157-68. doi: 
            10.1016/j.chom.2015.07.001. Epub 2015 Jul 30.
  PUBMED    26235147
REFERENCE   2  (bases 1 to 6577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chom.2015.07.001"; journalName: "Cell Host Microbe"; 
            date: "2015-08-12- 12"; volume: "18"; issue: "2"; pages: "157-68"
COMMENT     Sequence Label: pcDNA3.1-mSTING
FEATURES             Location/Qualifiers
     source          1..6577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      230..818
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     719..738
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     972..989
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1106..1127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1142..1147,1148..1165,1166..1172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1180..1196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1185..1207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1204..1220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1204..1220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1269..1390
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1280..1299)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1334..1353
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1564..2358)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1675..1694)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2285..2304
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2425..2754)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2439..2574
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2493..2512)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     2739..2759
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(2768..3196)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2878..2899)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3090..3109
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    3242..3466
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3455..3472
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(4758..4777)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4759..4777)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(4822..5025)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4851..4871)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5026..5405
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     5577..5596
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5671..5775
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5776..5844,5845..6577,1..59)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5994..6013)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
       61 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
      121 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
      181 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
      241 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
      301 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
      361 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
      421 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
      481 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
      541 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
      601 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
      661 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
      721 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
      781 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
      841 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
      901 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
      961 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     1021 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     1081 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     1141 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     1201 acacaggaaa cagctatgac catgattacg ccaagctcta gctagaggtc gacggtatac
     1261 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     1321 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     1381 taaacaagtt ggggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc
     1441 agccggcgtc ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat
     1501 cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt catttcgaac cccagagtcc
     1561 cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc
     1621 gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc
     1681 acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc cacagtcgat
     1741 gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt
     1801 cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc ctggcgaaca gttcggctgg
     1861 cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg
     1921 agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc
     1981 aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag
     2041 gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc
     2101 ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag
     2161 ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag
     2221 aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg
     2281 ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa
     2341 tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcag atccgaaaat
     2401 ggatatacaa gctcccggga gctttttgca aaagcctagg cctccaaaaa agcctcctca
     2461 ctacttctgg aatagctcag aggcagaggc ggcctcggcc tctgcataaa taaaaaaaat
     2521 tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg
     2581 agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca tacttctgcc
     2641 tgctggggag cctggggact ttccacacct ggttgctgac taattgagat gcatgctttg
     2701 catacttctg cctgctgggg agcctgggga ctttccacac cctaactgac acacattcca
     2761 cagaattaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     2821 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     2881 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     2941 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     3001 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     3061 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     3121 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     3181 ccgctacagg gcgcgtgggg atacccccta gagccccagc tggttctttc cgcctcagaa
     3241 gccatagagc ccaccgcatc cccagcatgc ctgctattgt cttcccaatc ctcccccttg
     3301 ctgtcctgcc ccaccccacc ccccagaata gaatgacacc tactcagaca atgcgatgca
     3361 atttcctcat tttattagga aaggacagtg ggagtggcac cttccagggt caaggaaggc
     3421 acgggggagg ggcaaacaac agatggctgg caactagaag gcacagtcga ggctgatcag
     3481 cgggtttaaa cgggccctct agactcgagc ggccgccact gtgctggata tctgcagaat
     3541 tctcagatga ggtcagtgcg gagtgggaga ggctgatcca taccactgat gaggagtctt
     3601 ggctcttggg acagtacgga gggaggaggt gccactgagg tcatgggggc attcatggta
     3661 acctcctcct tttcttcctg acgaatgtgc cggagcacct cctgagacag tgagaaactg
     3721 tttccgtctg tgggttcttg gtagacaatg aggcggcagt tatttcgaga ctcggggaca
     3781 tcttccagga tttcctcaag tgtccggcag aagagtttag cctgctcaag ccgatcctcc
     3841 cgactgaagc cagctttggc atcctgtgac atggcaaaca gggtctgcaa gggggtggcg
     3901 tactccagga tacagacgcc tgctggctgt ccgttctcca gaatctcgta gacgctgttg
     3961 gaataaaccc gattcttgat gccagcacgg tcgatgtttt gctggggcag catatctcgg
     4021 aatcgaatgt tggggtcaac tacactcagg ttgtcaggca ccccacagtc caatggaaag
     4081 aggatgtaca gtcttcggct ccctgcacca ctgagcatgt tgttatgtag ctgattgaac
     4141 attcggatcc gggcctggag ccctggtaag atcaaccgca agtacccaat gtagtatgac
     4201 caggccagcc cgtgggcaac atttaacttc ttttcttcac agactgcaga gacttccgct
     4261 ggagtcaagc tctgaaggcc caggagcatg cttagggact tatagaggac cagaaggcca
     4321 aacatccaac tgaggtatat gtcagcagtg ttttggagga aatagaaata agacgatagt
     4381 agaatcatag ccatacagtg gatggggcat cccaggcagg cgcgcacagc cttccagtag
     4441 ctgccctggt acctggactg gacatggcac agctcttcag ccagacagca gaggtttttc
     4501 aacagtagtc caagttcgtg cgaggctagg tgaagtgcta ggtacttcag agtgtgattt
     4561 ggtggatcct ttgccaccca aaggatcatc aggctggcca ccagaaagat gagggctaca
     4621 tatttggagc ggtgacctct gggccgtggg atggctggat gcaggttgga gtatggcatg
     4681 gtgaattcca ccacactgga ctagtggatc cgagctcggt accaagctta agtttaaacg
     4741 ctagccagct tgggtctccc tatagtgagt cgtattaatt tcgataagcc agtaagcagt
     4801 gggttctcta gttagccaga gagctctgct tatatagacc tcccaccgta cacgcctacc
     4861 gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc gttgattttg
     4921 gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc
     4981 aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg taatagcgat
     5041 gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcata
     5101 atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg gcatatgata
     5161 cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt gacgtcaatg
     5221 gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca atgggcgggg
     5281 gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc ggaactccat
     5341 atatgggcta tgaactaatg accccgtaat tgattactat taataactag tcaataatca
     5401 atgtcaacgc gtatatctgg cccgtacatc gcgaagcagc gcaaaacgcc taaccctaag
     5461 cagattcttc atgcaattgt cggtcaagcc ttgccttgtt gtagcttaaa ttttgctcgc
     5521 gcactactca gcgacctcca acacacaagc agggagcaga tactggctta actatgcggc
     5581 atcagagcag attgtactga gagtgcacca taggggatcg ggagatctcc cgatccgtcg
     5641 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     5701 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     5761 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     5821 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     5881 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     5941 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     6001 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     6061 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     6121 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     6181 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     6241 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     6301 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     6361 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     6421 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     6481 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     6541 atcgtagtta tctacacgac ggggagtcag gcaacta
//
