LOCUS       sequence-424637-        6607 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient expression of STING.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6607)
  AUTHORS   Chu TT, Tu X, Yang K, Wu J, Repa JJ, Yan N
  TITLE     Tonic prime-boost of STING signalling mediates Niemann-Pick disease 
            type C.
  JOURNAL   Nature. 2021 Aug;596(7873):570-575. doi: 10.1038/s41586-021-03762-2.
            Epub 2021 Jul 21.
  PUBMED    34290407
REFERENCE   2  (bases 1 to 6607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-021-03762-2"; journalName: "Nature"; date: 
            "2021-08"; volume: "596"; issue: "7873"; pages: "570-575"
COMMENT     Sequence Label: pcDNA3.1-HA-mSTING
FEATURES             Location/Qualifiers
     source          1..6607
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1092..1109)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1098..1322
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1368..1796
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1455..1474)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1665..1686
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1805..1825)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1810..2139
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1990..2125
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2052..2071
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             2206..3000
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2260..2279)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2870..2889
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3174..3295
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3211..3230)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3265..3284
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3344..3360)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3344..3360)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3357..3379)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3368..3384
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3392..3398,3399..3416,3417..3422))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3437..3458
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3575..3592)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3746..4334)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3826..3845)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4505..5296,5297..5365))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5128..5147
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5366..5470)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5545..5564)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5736..6115
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6116..6319
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6270..6290
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6364..6383
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6364..6382
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6465..6491
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 accaaatcac actctgaagt acctagcact tcacctagcc tcgcacgaac ttggactact
       61 gttgaaaaac ctctgctgtc tggctgaaga gctgtgccat gtccagtcca ggtaccaggg
      121 cagctactgg aaggctgtgc gcgcctgcct gggatgcccc atccactgta tggctatgat
      181 tctactatcg tcttatttct atttcctcca aaacactgct gacatatacc tcagttggat
      241 gtttggcctt ctggtcctct ataagtccct aagcatgctc ctgggccttc agagcttgac
      301 tccagcggaa gtctctgcag tctgtgaaga aaagaagtta aatgttgccc acgggctggc
      361 ctggtcatac tacattgggt acttgcggtt gatcttacca gggctccagg cccggatccg
      421 aatgttcaat cagctacata acaacatgct cagtggtgca gggagccgaa gactgtacat
      481 cctctttcca ttggactgtg gggtgcctga caacctgagt gtagttgacc ccaacattcg
      541 attccgagat atgctgcccc agcaaaacat cgaccgtgct ggcatcaaga atcgggttta
      601 ttccaacagc gtctacgaga ttctggagaa cggacagcca gcaggcgtct gtatcctgga
      661 gtacgccacc cccttgcaga ccctgtttgc catgtcacag gatgccaaag ctggcttcag
      721 tcgggaggat cggcttgagc aggctaaact cttctgccgg acacttgagg aaatcctgga
      781 agatgtcccc gagtctcgaa ataactgccg cctcattgtc taccaagaac ccacagacgg
      841 aaacagtttc tcactgtctc aggaggtgct ccggcacatt cgtcaggaag aaaaggagga
      901 ggttaccatg aatgccccca tgacctcagt ggcacctcct ccctccgtac tgtcccaaga
      961 gccaagactc ctcatcagtg gtatggatca gcctctccca ctccgcactg acctcatctg
     1021 agaattctgc agatatccag cacagtggcg gccgctcgag tctagagggc ccgtttaaac
     1081 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     1141 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     1201 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     1261 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     1321 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     1381 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     1441 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     1501 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     1561 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     1621 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     1681 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     1741 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     1801 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     1861 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     1921 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     1981 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     2041 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     2101 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     2161 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     2221 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     2281 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     2341 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     2401 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     2461 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     2521 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     2581 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     2641 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     2701 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     2761 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     2821 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     2881 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     2941 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     3001 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     3061 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     3121 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     3181 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     3241 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     3301 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     3361 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     3421 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     3481 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     3541 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     3601 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     3661 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     3721 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     3781 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     3841 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     3901 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     3961 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     4021 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     4081 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     4141 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     4201 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     4261 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     4321 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     4381 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     4441 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     4501 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     4561 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     4621 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     4681 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     4741 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     4801 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     4861 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     4921 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     4981 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     5041 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     5101 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     5161 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     5221 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     5281 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     5341 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     5401 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     5461 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
     5521 cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     5581 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
     5641 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc
     5701 gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta
     5761 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     5821 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     5881 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     5941 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     6001 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     6061 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
     6121 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
     6181 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
     6241 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
     6301 ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga
     6361 aattaatacg actcactata gggagaccca agctggctag cgtttaaact taagcttggt
     6421 accgagctcg gatccactag tccagtgtgg tggaattcac catgtaccca tacgacgtac
     6481 cagattacgc tatgccatac tccaacctgc atccagccat cccacggccc agaggtcacc
     6541 gctccaaata tgtagccctc atctttctgg tggccagcct gatgatcctt tgggtggcaa
     6601 aggatcc
//
