LOCUS       sequence-424638-        6604 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient expression of gain-of-function STING mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6604)
  AUTHORS   Dobbs N, Burnaevskiy N, Chen D, Gonugunta VK, Alto NM, Yan N
  TITLE     STING Activation by Translocation from the ER Is Associated with 
            Infection and Autoinflammatory Disease.
  JOURNAL   Cell Host Microbe. 2015 Aug 12;18(2):157-68. doi: 
            10.1016/j.chom.2015.07.001. Epub 2015 Jul 30.
  PUBMED    26235147
REFERENCE   2  (bases 1 to 6604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chom.2015.07.001"; journalName: "Cell Host Microbe"; 
            date: "2015-08-12- 12"; volume: "18"; issue: "2"; pages: "157-68"
COMMENT     Sequence Label: pcDNA3.1-HA-mSTING (N153S)
FEATURES             Location/Qualifiers
     source          1..6604
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     588..607
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(826..930)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1005..1024)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1196..1575
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1576..1779
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1730..1750
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1824..1843
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1824..1842
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1925..1951
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(3156..3173)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3162..3386
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3432..3860
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3519..3538)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3729..3750
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3869..3889)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3874..4203
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4054..4189
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4116..4135
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4270..5064
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4324..4343)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4934..4953
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5238..5359
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5275..5294)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5329..5348
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5408..5424)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5408..5424)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5421..5443)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5432..5448
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5456..5462,5463..5480,5481..5486))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5501..5522
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5639..5656)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5810..6398)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5890..5909)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6569..6604,1..756,757..825))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
       61 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
      121 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
      181 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
      241 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
      301 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
      361 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
      421 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
      481 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
      541 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
      601 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
      661 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
      721 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
      781 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
      841 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
      901 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
      961 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
     1021 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
     1081 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
     1141 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
     1201 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     1261 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     1321 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     1381 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     1441 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     1501 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     1561 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     1621 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     1681 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     1741 ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc
     1801 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agctggctag
     1861 cgtttaaact taagcttggt accgagctcg gatccactag tccagtgtgc tggaattcac
     1921 catgtaccca tacgacgtac cagattacgc tccatactcc aacctgcatc cagccatccc
     1981 acggcccaga ggtcaccgct ccaaatatgt agccctcatc tttctggtgg ccagcctgat
     2041 gatcctttgg gtggcaaagg atccaccaaa tcacactctg aagtacctag cacttcacct
     2101 agcctcgcac gaacttggac tactgttgaa aaacctctgc tgtctggctg aagagctgtg
     2161 ccatgtccag tccaggtacc agggcagcta ctggaaggct gtgcgcgcct gcctgggatg
     2221 ccccatccac tgtatggcta tgattctact atcgtcttat ttctatttcc tccaaaacac
     2281 tgctgacata tacctcagtt ggatgtttgg ccttctggtc ctctataagt ccctaagcat
     2341 gctcctgggc cttcagagct tgactccagc ggaagtctct gcagtctgtg aagaaaagaa
     2401 gttaagtgtt gcccacgggc tggcctggtc atactacatt gggtacttgc ggttgatctt
     2461 accagggctc caggcccgga tccgaatgtt caatcagcta cataacaaca tgctcagtgg
     2521 tgcagggagc cgaagactgt acatcctctt tccattggac tgtggggtgc ctgacaacct
     2581 gagtgtagtt gaccccaaca ttcgattccg agatatgctg ccccagcaaa acatcgaccg
     2641 tgctggcatc aagaatcggg tttattccaa cagcgtctac gagattctgg agaacggaca
     2701 gccagcaggc gtctgtatcc tggagtacgc cacccccttg cagaccctgt ttgccatgtc
     2761 acaggatgcc aaagctggct tcagtcggga ggatcggctt gagcaggcta aactcttctg
     2821 ccggacactt gaggaaatcc tggaagatgt ccccgagtct cgaaataact gccgcctcat
     2881 tgtctaccaa gaacccacag acggaaacag tttctcactg tctcaggagg tgctccggca
     2941 cattcgtcag gaagaaaagg aggaggttac catgaatgcc cccatgacct cagtggcacc
     3001 tcctccctcc gtactgtccc aagagccaag actcctcatc agtggtatgg atcagcctct
     3061 cccactccgc actgacctca tctgagaatt ctgcagatat ccagcacagt ggcggccgct
     3121 cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     3181 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     3241 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     3301 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     3361 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     3421 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     3481 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     3541 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     3601 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     3661 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     3721 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     3781 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     3841 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     3901 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     3961 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4021 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     4081 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     4141 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     4201 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga
     4261 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     4321 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     4381 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     4441 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     4501 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     4561 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     4621 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     4681 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     4741 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     4801 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     4861 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     4921 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     4981 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     5041 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     5101 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     5161 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     5221 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     5281 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5341 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     5401 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     5461 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     5521 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     5581 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     5641 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     5701 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     5761 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     5821 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     5881 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     5941 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6001 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6061 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6121 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6181 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6241 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6301 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     6361 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6421 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     6481 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     6541 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     6601 cagc
//
