LOCUS       sequence-424640-        6580 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient expression of gain-of-function STING mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6580)
  AUTHORS   Dobbs N, Burnaevskiy N, Chen D, Gonugunta VK, Alto NM, Yan N
  TITLE     STING Activation by Translocation from the ER Is Associated with 
            Infection and Autoinflammatory Disease.
  JOURNAL   Cell Host Microbe. 2015 Aug 12;18(2):157-68. doi: 
            10.1016/j.chom.2015.07.001. Epub 2015 Jul 30.
  PUBMED    26235147
REFERENCE   2  (bases 1 to 6580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chom.2015.07.001"; journalName: "Cell Host Microbe"; 
            date: "2015-08-12- 12"; volume: "18"; issue: "2"; pages: "157-68"
COMMENT     Sequence Label: pcDNA3.1-hSTING (N154S)
FEATURES             Location/Qualifiers
     source          1..6580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      68..656
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     557..576
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     810..827
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    944..965
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(980..985,986..1003,1004..1010)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1018..1034
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1023..1045
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1042..1058
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1042..1058
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1107..1228
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1118..1137)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1172..1191
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1402..2196)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1513..1532)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2123..2142
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2263..2592)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2277..2412
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2331..2350)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     2577..2597
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(2606..3034)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2716..2737)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2928..2947
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    3080..3304
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3293..3310
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(4599..4618)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4600..4618)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(4663..4866)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4692..4712)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4867..5246
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     5418..5437
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5512..5616
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5617..5685,5686..6477)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5835..5854)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
       61 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
      121 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
      181 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
      241 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
      301 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
      361 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
      421 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
      481 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
      541 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
      601 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
      661 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
      721 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
      781 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
      841 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
      901 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
      961 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     1021 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagctctagc
     1081 tagaggtcga cggtatacag acatgataag atacattgat gagtttggac aaaccacaac
     1141 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     1201 aaccattata agctgcaata aacaagttgg ggtgggcgaa gaactccagc atgagatccc
     1261 cgcgctggag gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat
     1321 agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca
     1381 tttcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg
     1441 ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc
     1501 aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc
     1561 cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa
     1621 gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct
     1681 ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac
     1741 aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa
     1801 tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac
     1861 tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag
     1921 cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt
     1981 cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg caccggacag
     2041 gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc
     2101 agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc
     2161 cggagaacct gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc
     2221 ttgatcagat ccgaaaatgg atatacaagc tcccgggagc tttttgcaaa agcctaggcc
     2281 tccaaaaaag cctcctcact acttctggaa tagctcagag gcagaggcgg cctcggcctc
     2341 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt
     2401 taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcat
     2461 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta
     2521 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacaccc
     2581 taactgacac acattccaca gaattaattc gcgttaaatt tttgttaaat cagctcattt
     2641 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     2701 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     2761 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     2821 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     2881 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     2941 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     3001 cccgccgcgc ttaatgcgcc gctacagggc gcgtggggat accccctaga gccccagctg
     3061 gttctttccg cctcagaagc catagagccc accgcatccc cagcatgcct gctattgtct
     3121 tcccaatcct cccccttgct gtcctgcccc accccacccc ccagaataga atgacaccta
     3181 ctcagacaat gcgatgcaat ttcctcattt tattaggaaa ggacagtggg agtggcacct
     3241 tccagggtca aggaaggcac gggggagggg caaacaacag atggctggca actagaaggc
     3301 acagtcgagg ctgatcagcg ggtttaaacg ggccctctag actcgagcgg ccgccactgt
     3361 gctggatatc tgcagaattc tcaagagaaa tccgtgcgga gagggagggg cttttccatt
     3421 ccactgatga ggagctcagg ctcttgggac atcgtggagg tactgggcac cgctgaggtc
     3481 ttcaagctgc ccacagtaac ctcttccttt tcctcctgcc gcaggtgccg gagaacctcc
     3541 tgggacagcg agaagctgct gtcatctgca ggttcctggt aggcaatgag gcggcagttg
     3601 ttctgagact caggggcatc tgccaggatg tcctcaagtg tccggcagaa gagtttggcc
     3661 tgctcaagcc tatcctcccg gctaaagcca gcttgactgt attgtgacat ggcaaacaaa
     3721 gtctgcaagg gggtggcgta ctccaggaca caggtgcccg cccgctgccc gttctccaga
     3781 agctcataga tgctgttgct gtaaacccga tccttgatgc cagcacggtc accggtctgc
     3841 tggggcagtt tatccaggaa gcgaatgttg gggtcagcca tactcaggtt atcaggcacc
     3901 ccacagtcca atgggaggag aatatacagc cgctggctca ctgcaccccg tagcaggttg
     3961 ttgtaatgct gattgtaagt tcgaatccgg gcctggagct ctggcaggat cagccgcaga
     4021 tatccgatgt aatatgacca tgccagccca tgggccacgc tgaaattccc tttttcacac
     4081 actgcagaga tctcagctgg ggccaggccc ttgaggccca ggaggatgtt cagtgcctgc
     4141 gagaggccca ggagggcaag catccaagtg aagggcgggc cgaccgcatt tgggagggag
     4201 tagtagaaat agatggacag cagcaacagg gccccacggc ggagggggca gcccaggcag
     4261 gcccgcacag tcctccagta gctgccccgg tacctggagt ggatgtggcg cagctcctca
     4321 gccaggctgc agaccccgtt taacagcagt cccagctgca gggaggctag gtggagcacc
     4381 aggtaccgga gagtgtgctc tggtggctct cctagccccc aaagggtcac caggcaggca
     4441 ctcagcagaa ccaaggctgc cttctgggcc ccgtgacccc tgggacacgg gatggatgga
     4501 tgcaggctgg agtggggcat ggtgaattcc accacactgg actagtggat ccgagctcgg
     4561 taccaagctt aagtttaaac gctagccagc ttgggtctcc ctatagtgag tcgtattaat
     4621 ttcgataagc cagtaagcag tgggttctct agttagccag agagctctgc ttatatagac
     4681 ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt tacgacattt
     4741 tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa tggggtggag
     4801 acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact gccaaaaccg
     4861 catcaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca
     4921 taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg
     4981 gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata
     5041 ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat
     5101 tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag
     5161 ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa ttgattacta
     5221 ttaataacta gtcaataatc aatgtcaacg cgtatatctg gcccgtacat cgcgaagcag
     5281 cgcaaaacgc ctaaccctaa gcagattctt catgcaattg tcggtcaagc cttgccttgt
     5341 tgtagcttaa attttgctcg cgcactactc agcgacctcc aacacacaag cagggagcag
     5401 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc ataggggatc
     5461 gggagatctc ccgatccgtc gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     5521 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5581 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5641 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5701 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5761 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5821 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     5881 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5941 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     6001 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     6061 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     6121 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     6181 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     6241 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     6301 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     6361 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     6421 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     6481 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6541 aggatctagg tgaagatcct ttttgataat ctcatgacca
//
