LOCUS       sequence-424641-        6580 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient expression of gain-of-function STING mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6580)
  AUTHORS   Dobbs N, Burnaevskiy N, Chen D, Gonugunta VK, Alto NM, Yan N
  TITLE     STING Activation by Translocation from the ER Is Associated with 
            Infection and Autoinflammatory Disease.
  JOURNAL   Cell Host Microbe. 2015 Aug 12;18(2):157-68. doi: 
            10.1016/j.chom.2015.07.001. Epub 2015 Jul 30.
  PUBMED    26235147
REFERENCE   2  (bases 1 to 6580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chom.2015.07.001"; journalName: "Cell Host Microbe"; 
            date: "2015-08-12- 12"; volume: "18"; issue: "2"; pages: "157-68"
COMMENT     Sequence Label: pcDNA3.1-hSTING (V155M)
FEATURES             Location/Qualifiers
     source          1..6580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1285..1304)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1286..1304)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1349..1552)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1378..1398)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1553..1932
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     2104..2123
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        2198..2302
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2303..2371,2372..3163)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2521..2540)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3334..3922
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3823..3842
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4076..4093
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4210..4231
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4246..4251,4252..4269,4270..4276)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4284..4300
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4289..4311
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4308..4324
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4308..4324
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    4373..4494
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4384..4403)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4438..4457
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(4668..5462)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4779..4798)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5389..5408
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5529..5858)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5543..5678
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5597..5616)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     5843..5863
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(5872..6300)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5982..6003)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6194..6213
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    6346..6570
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     6559..6576
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 tcagcgggtt taaacgggcc ctctagactc gagcggccgc cactgtgctg gatatctgca
       61 gaattctcaa gagaaatccg tgcggagagg gaggggcttt tccattccac tgatgaggag
      121 ctcaggctct tgggacatcg tggaggtact gggcaccgct gaggtcttca agctgcccac
      181 agtaacctct tccttttcct cctgccgcag gtgccggaga acctcctggg acagcgagaa
      241 gctgctgtca tctgcaggtt cctggtaggc aatgaggcgg cagttgttct gagactcagg
      301 ggcatctgcc aggatgtcct caagtgtccg gcagaagagt ttggcctgct caagcctatc
      361 ctcccggcta aagccagctt gactgtattg tgacatggca aacaaagtct gcaagggggt
      421 ggcgtactcc aggacacagg tgcccgcccg ctgcccgttc tccagaagct catagatgct
      481 gttgctgtaa acccgatcct tgatgccagc acggtcaccg gtctgctggg gcagtttatc
      541 caggaagcga atgttggggt cagccatact caggttatca ggcaccccac agtccaatgg
      601 gaggagaata tacagccgct ggctcactgc accccgtagc aggttgttgt aatgctgatt
      661 gtaagttcga atccgggcct ggagctctgg caggatcagc cgcagatatc cgatgtaata
      721 tgaccatgcc agcccatggg ccatgttgaa attccctttt tcacacactg cagagatctc
      781 agctggggcc aggcccttga ggcccaggag gatgttcagt gcctgcgaga ggcccaggag
      841 ggcaagcatc caagtgaagg gcgggccgac cgcatttggg agggagtagt agaaatagat
      901 ggacagcagc aacagggccc cacggcggag ggggcagccc aggcaggccc gcacagtcct
      961 ccagtagctg ccccggtacc tggagtggat gtggcgcagc tcctcagcca ggctgcagac
     1021 cccgtttaac agcagtccca gctgcaggga ggctaggtgg agcaccaggt accggagagt
     1081 gtgctctggt ggctctccta gcccccaaag ggtcaccagg caggcactca gcagaaccaa
     1141 ggctgccttc tgggccccgt gacccctggg acacgggatg gatggatgca ggctggagtg
     1201 gggcatggtg aattccacca cactggacta gtggatccga gctcggtacc aagcttaagt
     1261 ttaaacgcta gccagcttgg gtctccctat agtgagtcgt attaatttcg ataagccagt
     1321 aagcagtggg ttctctagtt agccagagag ctctgcttat atagacctcc caccgtacac
     1381 gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt
     1441 gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc
     1501 gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa
     1561 tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact
     1621 gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca
     1681 tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac
     1741 gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg
     1801 ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga
     1861 actccatata tgggctatga actaatgacc ccgtaattga ttactattaa taactagtca
     1921 ataatcaatg tcaacgcgta tatctggccc gtacatcgcg aagcagcgca aaacgcctaa
     1981 ccctaagcag attcttcatg caattgtcgg tcaagccttg ccttgttgta gcttaaattt
     2041 tgctcgcgca ctactcagcg acctccaaca cacaagcagg gagcagatac tggcttaact
     2101 atgcggcatc agagcagatt gtactgagag tgcaccatag gggatcggga gatctcccga
     2161 tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     2221 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     2281 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     2341 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     2401 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     2461 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     2521 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     2581 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     2641 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     2701 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     2761 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     2821 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     2881 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     2941 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     3001 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     3061 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     3121 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     3181 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     3241 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     3301 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3361 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     3421 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3481 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     3541 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     3601 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     3661 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     3721 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     3781 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     3841 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     3901 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     3961 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     4021 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     4081 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     4141 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     4201 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     4261 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     4321 tgaccatgat tacgccaagc tctagctaga ggtcgacggt atacagacat gataagatac
     4381 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     4441 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttggggtg
     4501 ggcgaagaac tccagcatga gatccccgcg ctggaggatc atccagccgg cgtcccggaa
     4561 aacgattccg aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat ctcgtgatgg
     4621 caggttgggc gtcgcttggt cggtcatttc gaaccccaga gtcccgctca gaagaactcg
     4681 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
     4741 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct
     4801 atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg
     4861 ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac gagatcctcg
     4921 ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc
     4981 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg
     5041 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc
     5101 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga
     5161 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg
     5221 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc
     5281 tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc
     5341 gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag
     5401 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc
     5461 atgcgaaacg atcctcatcc tgtctcttga tcagatccga aaatggatat acaagctccc
     5521 gggagctttt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt ctggaatagc
     5581 tcagaggcag aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc
     5641 ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggact
     5701 atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg
     5761 gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
     5821 ggggagcctg gggactttcc acaccctaac tgacacacat tccacagaat taattcgcgt
     5881 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     5941 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     6001 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     6061 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     6121 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     6181 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     6241 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     6301 ggggataccc cctagagccc cagctggttc tttccgcctc agaagccata gagcccaccg
     6361 catccccagc atgcctgcta ttgtcttccc aatcctcccc cttgctgtcc tgccccaccc
     6421 caccccccag aatagaatga cacctactca gacaatgcga tgcaatttcc tcattttatt
     6481 aggaaaggac agtgggagtg gcaccttcca gggtcaagga aggcacgggg gaggggcaaa
     6541 caacagatgg ctggcaacta gaaggcacag tcgaggctga
//
