LOCUS       sequence-424644-       10531 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient expression of GCC2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10531)
  AUTHORS   Tu X, Chu TT, Jeltema D, Abbott K, Yang K, Xing C, Han J, Dobbs N, 
            Yan N
  TITLE     Interruption of post-Golgi STING trafficking activates tonic 
            interferon signaling.
  JOURNAL   Nat Commun. 2022 Nov 15;13(1):6977. doi: 10.1038/s41467-022-33765-0.
  PUBMED    36379959
REFERENCE   2  (bases 1 to 10531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-11-15"; volume: "13(1):6977. doi"; pages: " 
            10.1038/s41467-022-33765-0"
COMMENT     Sequence Label: pcDNA3.1-mGCC2-FLAG
FEATURES             Location/Qualifiers
     source          1..10531
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     185..205
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(214..642)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(324..345)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     536..555
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    688..912
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     901..918
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(6158..6177)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6159..6177)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(6222..6425)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6251..6271)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6426..6805
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     6977..6996
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7071..7175
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7176..7244,7245..8036)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7394..7413)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8207..8795
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8696..8715
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8949..8966
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9083..9104
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9119..9124,9125..9142,9143..9149)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9157..9173
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9162..9184
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9181..9197
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9181..9197
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    9246..9367
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9257..9276)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9311..9330
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(9541..10335)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9652..9671)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     10262..10281
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(10402..10531,1..200))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(10416..10531,1..20)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10470..10489)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg
       61 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acacctggtt
      121 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
      181 ccacacccta actgacacac attccacaga attaattcgc gttaaatttt tgttaaatca
      241 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      301 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      361 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      421 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      481 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      541 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      601 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtggggatac cccctagagc
      661 cccagctggt tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc
      721 tattgtcttc ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat
      781 gacacctact cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag
      841 tggcaccttc cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac
      901 tagaaggcac agtcgaggct gatcagcggg tttaaacggg ccctctagac tcgagcggcc
      961 gccactgtgc tggatatctg cagaattcct acntntnant ncngntncng ntncnantnc
     1021 ncntntngnt nanantncnt cgcagtccag accagctatg cagataggac gcccatccag
     1081 aggaacgaga agcactttct tcctcacctt gagcaactgt ggcaagcttt cccttttcct
     1141 cagggctgag ctgcagcatg gtgtctatca caggcagaag cctctctctt tcactacccg
     1201 gcttcaggaa aatgaaccgc agcaagacat tcttcaagta ctccaggtta gccacagact
     1261 tctcacgctc ttggtttctt tccagtcttc ttatttcact cttgagaagc ttaatttgct
     1321 ccataaggat agcgttggta gcctctgtct cccgaagcag gccgtttaag tgatctgcac
     1381 tcttcgtggt ggagctgagc ttctcagcta attcttcttt ggtaaattca ttatgccaca
     1441 aggaggtctc tgcaaggcgt tcaagcttgg tgtcaggaga gctaaggagc tgctctaagg
     1501 actgaatgtg tgtgcctgcc gacgacacag actcgctgtc agttgtctcc atgccctccc
     1561 cctcctcgcg ggccacagtg tgcatgtcca gaagggggag gtctgtggtt cttctttctc
     1621 ggaggctttt cgaaggttga tgggaagaag cagggcttct tgtggatggc tcactcttga
     1681 gctggaagag ctgagcttcc agcttggaga gctggtgctg cagagtctcc actgtctttc
     1741 tgtgctcatc ctgcaagtgt cgaacttggt ctcggaagct ggtacggagt gcttcattct
     1801 gggatgttag ctgacacatt gtctgcgagt gctcagactt catcatggtg ttctcagatt
     1861 gaactgaaca tagcttttcc cgaagttctg cctctttggt cacagtttcc tgcagcatcc
     1921 ggttgtgcct ctctagcaga gtgtcgtgct cagcctgcag ggtctggtac tccgatacac
     1981 tgatttgtaa gctattttgg ctgtcctgta acttgatttt cagttggtca atcagcattt
     2041 ccaggtgttc cctttcttgt ttagctccct cagtttcaac ctgagacaca gatttatttt
     2101 tctgttgctt tagaacgtta tgaactcgga ctttgtagct ctcaaattca gaggtgacag
     2161 cagcttgttc tgccttggca gcgcgactct cctcctgcag ggccaccacc ctctgctggt
     2221 atgcactcag tgtgcgctgg tgctgttccg cagaagtctt cagatgctca tggattttgt
     2281 gtttctcaga tgtcatctca gccagctgaa ttttatagac ttctacttgc tgctggcttg
     2341 cttccagctc accctttaaa gatgcctgga gtagtaagtg atcggtttcc gcttgctttg
     2401 catctgccag ctcctttttg gttttcacaa gcagctgctt gattttagtg ttcttttctt
     2461 catagtgctg tactgacgac tgcagtgaag ttatttcttc ttgtagggtt tcttgtttct
     2521 ctttctgaat tttcatttct tgctccaaat cttctattgt actgttttta tttgttaact
     2581 tttgatttag ttctttcatc aaacgttcgt aatctgctat ttccatattc atcaatgtgg
     2641 tctgttgggc atccttttta accagctcta attcttgcat tgttttctga agctgcttct
     2701 tttccttttg aaattggagt tggagttctt cgagctcatt tacagtactg acatgttcct
     2761 taattttctg ctctttctga agtttttcag cgtccagttc tttttctact actcctttgg
     2821 ctttctggac ttctaagatc tgtgcttcta acaacttggc attagcctgc agggtctcaa
     2881 ttcgagccag gagatcctcg ttgtctgaag ttagtctttc atactggcaa gtcgaatttc
     2941 ttaattgttt ggtaaggtct tctatatgac gttcaaaatt atgagcacgt tctttttcca
     3001 catccagctg ttctgactgc ttatcatatt ctaacagaag actcttatag ctctctgctc
     3061 cttggaggaa ctccttcata gaagcagaca ggcggtcctt ttctgaccgc acggattcca
     3121 gttcttcccg tagtgtctgg gcctctttcc tgttagaatc tagttctttc tttgctttca
     3181 cagcaactag ttttatctta cttattttct catccttgtc tttggattct ttttccagac
     3241 tttctatttt ttcttccagc tcttttacca atgaaagttg atcatttttt acagaaggcg
     3301 atttctctaa ggaatccttt agcagtatca attctgctct cacatcttgg agttcagatt
     3361 ccctctgttt aagcagtagc cttaggtttt catgttctat tacaagaacc ttttcatcat
     3421 ggacttcatt tctgctgtgt aatgtctgag ataattcttc aacctgagtt gaaagagtct
     3481 ggttttcaag gcttgctttc tcattagtat tcttcatttc taaaagatca agttggaggg
     3541 cctcttttgc tgatgtgact gcatctaatt cttttcttag gagaattttc tcttgctcag
     3601 agtctcttac taaagtcctt agttcttcac actgaactgc actgtcctct tgaagacact
     3661 taatcttatt ttctaattct aatacccttg catcatattc aaagactaag ttatgttctt
     3721 cttcctttac tttcactaaa gattcaccta cagcttgtat aagtttgaca acatcttcct
     3781 cttcattttc ttctaaaatg cttgccctca gttgcttaag atactcatac aactgagttt
     3841 taacaaatga cttctgaact tgctcatttt caagcagttt atttaaatcg tctcgctcct
     3901 caaccattaa ctggagtttc ttttcgaatt cagcaacatg ttctttatgg gcaaaatctt
     3961 gttgagcctg aagtgcttct aaatccctgc tcaactgggc cttttctgaa tgtagtctat
     4021 tgttttcctc ataaagagac ttgattttag atgacagagc ttcttgatct tccgtcatag
     4081 ttttcatttg aaccgtaatg ctttggtcat tttgactctt ttctcttact agttcatcta
     4141 gtttccctgt taattcactt actctttgtc tcagttcact gttgtgctgg gcagcttgcc
     4201 ctgctgcttc cctgagttct acagacagtc tctgttccct ttcccatttc tgatggagat
     4261 catccatctc ttcatgggaa gttttgattt tacttataaa gtcatctttt tctttagcaa
     4321 gagaacttat ctttgcttct aattcttgta acatggtatc tttttgacca agactggcta
     4381 agtacatgct attatctgcc tgaaggttct ttatggtact ttcaagttct ggtactaatt
     4441 cttgttgaga taataatttt tcattttctc cttggaggcg attcacagtt tcaagaagag
     4501 catctttttc attaaaagca cttcttagct tctgctgaag ctcatgaaca tcagatgctt
     4561 gctgttgctt cattgtttca aactcttgac ttatttttcc agcagattct ccaagttctg
     4621 tttgtaaaat ttccatcatc tctcgtaaac tctcatagtt tagaactacc tcttgctttt
     4681 catgttgtag attttcacac tgttccttaa gaccttgtat ttcaaacatc aatgctaact
     4741 tttctttttc tgaacctgat ataaatgtct cacttagttc agagacttct ttctgatggt
     4801 gttcctgaag acgctgtatt tctttgttat gttgttccac agcacagcaa agttgcctat
     4861 ttaaatcttc tcgctcatat tttaattttt ctactacata gccctgttct tctttagcca
     4921 gcaacagttc attaatttta aactctaagt cttctcgttc atggaaaaac tcatccttta
     4981 tatgctgggc atccatttca agttttaact tgagattatt catataagtt acttcatctt
     5041 ttaagatact atgttgagat tccagttctt ttaatgaatc ttccaattgt ctgactttat
     5101 cacatactgt ttgttccgca gaaggatctt cctgtaaggc cacagagcaa ttctgactct
     5161 ccgggcttgc atctgaggcg acttcattta agttttccga ttcacattgg tatttctctg
     5221 gaatgttctc acttgcctca cactgtgtca ctctgttctc ttttagttta tttatttctg
     5281 cctcgtgttc tttagctgat gtttctattt gagacatcag ctcagtaact tcttctttgt
     5341 gtgtagctct cagctgcaaa atctctgctt ggaaagtatt aatatctttt tggtagtgct
     5401 gagccttgtc ctcaatgact ttctggagat gaataatctc ttgttgtttt tcatcactgg
     5461 tagcttctaa tttcttctct agacatgaaa tctgttcctc aaatgctgct gtgatgtttt
     5521 gaatctcttc ctgcagtttt ctcacgttat cttcagagtc actctgagac ttaagctgct
     5581 ctctcagctc tacttgctta tggacagctt cttccagttc tttttgtaag atggccgttt
     5641 ctttgctgtg ctctgagtgc actgccatca gctcattttt acagctttca atttctttta
     5701 cgtagtttct atgtgattgt tcaaactctt tgtgtgtctc ttccagttta gttacagaat
     5761 cctcaacctc ttgcttcatt ttaacatttt cttttttcag acatagacac tgttgttcag
     5821 tctctgcttt ttccagaaga agagcatcca agcgttcagt tagtacctta ataatatcat
     5881 cagttcctcc atcaacaggt tttgatttga gttcttcaac ttctttgtct aattctgtac
     5941 accttgcttt ggctttctgc aggagcatca tttgcttctt ggcaaacttg atgagatctt
     6001 ctctgggcaa cgtttctagc ttggatttcg gggtcccgga gggtgctgca gctacggcat
     6061 ctggagcaga atcctccatg gtgaattcca ccacactgga ctagtggatc cgagctcggt
     6121 accaagctta agtttaaacg ctagccagct tgggtctccc tatagtgagt cgtattaatt
     6181 tcgataagcc agtaagcagt gggttctcta gttagccaga gagctctgct tatatagacc
     6241 tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt
     6301 ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga
     6361 cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc
     6421 atcaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat
     6481 aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg
     6541 ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac
     6601 tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt
     6661 attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt
     6721 tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat
     6781 taataactag tcaataatca atgtcaacgc gtatatctgg cccgtacatc gcgaagcagc
     6841 gcaaaacgcc taaccctaag cagattcttc atgcaattgt cggtcaagcc ttgccttgtt
     6901 gtagcttaaa ttttgctcgc gcactactca gcgacctcca acacacaagc agggagcaga
     6961 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca taggggatcg
     7021 ggagatctcc cgatccgtcg acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     7081 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     7141 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     7201 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     7261 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     7321 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     7381 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     7441 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     7501 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     7561 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     7621 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     7681 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     7741 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     7801 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     7861 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     7921 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     7981 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     8041 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     8101 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     8161 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     8221 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     8281 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     8341 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     8401 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     8461 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     8521 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     8581 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     8641 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     8701 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     8761 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     8821 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     8881 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     8941 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     9001 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     9061 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     9121 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     9181 caggaaacag ctatgaccat gattacgcca agctctagct agaggtcgac ggtatacaga
     9241 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     9301 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     9361 acaagttggg gtgggcgaag aactccagca tgagatcccc gcgctggagg atcatccagc
     9421 cggcgtcccg gaaaacgatt ccgaagccca acctttcata gaaggcggcg gtggaatcga
     9481 aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc
     9541 tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat
     9601 accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg
     9661 ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa
     9721 tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac
     9781 gacgagatcc tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc
     9841 gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt
     9901 acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag
     9961 cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg
    10021 agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc
    10081 agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg
    10141 cgctgcctcg tcctgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac
    10201 cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg
    10261 tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc
    10321 atcttgttca atcatgcgaa acgatcctca tcctgtctct tgatcagatc cgaaaatgga
    10381 tatacaagct cccgggagct ttttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta
    10441 cttctggaat agctcagagg cagaggcggc ctcggcctct gcataaataa aaaaaattag
    10501 tcagccatgg ggcggagaat gggcggaact g
//
