LOCUS       sequence-424669-        5663 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human DCAF13. C-terminal HiBiT 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5663)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 5663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-C_DCAF13:1-445
FEATURES             Location/Qualifiers
     source          1..5663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2726..2847
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2737..2756)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(2962..2995)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3005..3052
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3084..3878
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3138..3157)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3748..3767
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(4036..4624)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4116..4135)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4750..4798
                     /note="synthetic polyadenylation signal"
     CDS             complement(4822..5220)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      5217..5226
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5251..5608)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5265..5400
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        5417..5608
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     5565..5585
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggcga tcgcaacaag cttcgggaag
     1201 atctggagct agcggcgggg gatccggtgg gaattcgaca ccatgaaggt gaagatgctg
     1261 agccggaatc cggacaatta tgtccgcgaa accaagttgg acttacagag agttccaaga
     1321 aactacgatc ctgctttaca tccttttgag gtcccacgag aatatataag agctttaaat
     1381 gctaccaaac tggaacgagt atttgcaaaa ccattccttg cttcgctgga tggtcaccgt
     1441 gatggagtca attgcttggc aaagcatcca gagaagctgg ctactgtcct ttctgggtcg
     1501 tgtgatggag aggttagaat ttggaatcta actcagcgga attgtatccg tacaatacaa
     1561 gcacatgaag gctttgtacg aggaatatgt actcgctttt gtgggacttc ttttttcact
     1621 gttggtgatg acaaaactgt gaagcagtgg aaaatggatg ggccaggcta tggagacgag
     1681 gaagagccat tacatacaat attaggaaag acagtgtata ctgggattga tcatcactgg
     1741 aaagaagctg tttttgccac atgtggacag caagtagaca tttgggatga acaaagaact
     1801 aatcctatat gttcaatgac ctggggattt gacagtataa gtagtgttaa atttaaccca
     1861 attgagacat ttctcttggg aagttgtgca tctgacagga atatagtact gtacgatatg
     1921 aggcaagcta ctcctttgaa aaaggttatc ttagatatga gaacaaatac aatctgttgg
     1981 aaccctatgg aagctttcat ttttacagca gcaaatgaag attataactt atatactttt
     2041 gatatgcgtg cactggacac tcctgtaatg gtccatatgg atcatgtatc tgcagtgctt
     2101 gatgtggatt actctcccac tgggaaggag tttgtgtctg ctagtttcga taaatctatt
     2161 cgaatctttc ctgtagacaa aagtcgaagc agggaggtat atcatacaaa gagaatgcaa
     2221 catgttatct gtgtaaaatg gacttctgac agcaagtata ttatgtgtgg atctgatgaa
     2281 atgaacattc gcctgtggaa agctaatgct tctgaaaaat tgggtgtgct tacatcacga
     2341 gaaaaagcag ccaaggatta taaccagaaa ttgaaggaga aatttcagca ttatcctcat
     2401 ataaaacgta tagctcgtca tcgacatcta ccaaaatcta tctatagcca gattcaggaa
     2461 cagcgcatca tgaaagaagc tcgtcgacga aaggaagtga atcgtattaa acacagcaag
     2521 cctggatctg tgccacttgt gtcagagaag aagaaacacg tagtggcagt tgtaaaaggc
     2581 tcgagcggtg ggagctccgg tgtgagcggc tggcggctgt tcaagaagat tagctaagtt
     2641 taaacggccg cgactctaga ctgcaggcat gcaagctgat ttggctgctg ccaccgctga
     2701 gcaaggccgc ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact
     2761 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     2821 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     2881 gttcaggggg agatgtggga ggttttttta agcaagtaaa acctctacaa atgtggtaaa
     2941 atcttaatta agacttctaa tataacttcg tatagcatac attatacgaa gttatggttc
     3001 cactgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
     3061 gaactaaacc caggaggcag atcatgattg aacaagatgg attgcacgca ggttctccgg
     3121 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     3181 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     3241 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
     3301 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     3361 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     3421 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     3481 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     3541 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     3601 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     3661 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     3721 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     3781 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     3841 gcatcgcctt ctatcgcctt cttgacgagt tcttctgatt cgaaatgacc gaccaagcga
     3901 cgcccaaccg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     3961 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     4021 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     4081 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     4141 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     4201 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     4261 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     4321 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     4381 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     4441 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     4501 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     4561 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatt
     4621 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     4681 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttatagtc
     4741 cggaatcgtc acacaaaaaa ccaacacaca gatgtaatga aaataaagat attttattgc
     4801 ggccatcgtg atggctagag tttagccctc ccacacataa ccagagggca gcaattcacg
     4861 aatcccaact gccgtcggct gtccatcact gtccttcact atggctttga tcccaggatg
     4921 cagatcgaga agcacctgtc ggcaccgtcc gcaggggctc aagatgcccc tgttctcatt
     4981 tccgatcgcg acgatacaag tcaggttgcc agctgccgca gcagcagcag tgcccagcac
     5041 cacgagttct gcacaaggtc ccccagtaaa atgatataca ttgacaccag tgaagatgcg
     5101 gccgtcgcta gagagagctg cgctggcgac gctgtagtct tcagagatgg ggatgctgtt
     5161 gattgtagcc gttgctcttt caatgagggt ggattcttct tgagacaaag gcttggccat
     5221 ggtggcgcta gtgtcagaag aatcgagctt tttgcaaaag cctaggcctc caaaaaagcc
     5281 tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa
     5341 aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat
     5401 gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact
     5461 tctgcctgct ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat
     5521 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac
     5581 attccacagc tggttctttc cgcctcagaa ggtacctaac caagttcctc tttcagaggt
     5641 tatttcaggc catggtgctg cgc
//
