LOCUS       sequence-424685-        9073 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9073)
  AUTHORS   Zeng Y, Cullen BR
  TITLE     Sequence requirements for micro RNA processing and function in human
            cells.
  JOURNAL   RNA. 2003 Jan . 9(1):112-23.
  PUBMED    12554881
REFERENCE   2  (bases 1 to 9073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2003 
            Jan . 9(1):112-23."
COMMENT     Sequence Label: pCMV-miR21
FEATURES             Location/Qualifiers
     source          1..9073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(256..282)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(304..323)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(305..323)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(368..571)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        572..951
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     1123..1142
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1217..1321
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1322..1390,1391..2182)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2353..2941
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     enhancer        3605..3984
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3989..4192
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4674..4692)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4760..4864
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4865..4933,4934..5725)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6514..6531
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6648..6669
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6684..6689,6690..6707,6708..6714)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6722..6738
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6727..6749
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6746..6762
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6746..6762
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    6811..6932
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6822..6841)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6876..6895
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(7106..7900)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7217..7236)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7827..7846
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(7967..8296)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7981..8116
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8281..8301
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(8310..8738)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8420..8441)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8632..8651
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    8784..9008
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8997..9014
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        9034..9052
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     9034..9051
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(join(9065..9073,1..9))
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgatgatgc acgtgtagcg gccgcgaatt cggcggctga ggaagctgag gaggcggcgg
       61 cggcggcggc ggcggtggcg gctgttgctg ctgctgctgc tgctgctgct gctgctgctg
      121 ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctg
      181 ctgctgctgc tgctgctgct gctgctgctg ctgctggaag gacttgaggg actcgaaggc
      241 cttcataaag cttccagcgt agtctgggac gtcgtatggg tacatggtct ccagcttggg
      301 tctccctata gtgagtcgta ttaatttcga taagccagta agcagtgggt tctctagtta
      361 gccagagagc tctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca
      421 atggggcgga gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac
      481 tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac
      541 gcccattgat gtactgccaa aaccgcatca ccatggtaat agcgatgact aatacgtaga
      601 tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc
      661 atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg
      721 ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg
      781 gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc
      841 agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat gggctatgaa
      901 ctaatgaccc cgtaattgat tactattaat aactagtcaa taatcaatgt caacgcgtat
      961 atctggcccg tacatcgcga agcagcgcaa aacgcctaac cctaagcaga ttcttcatgc
     1021 aattgtcggt caagccttgc cttgttgtag cttaaatttt gctcgcgcac tactcagcga
     1081 cctccaacac acaagcaggg agcagatact ggcttaacta tgcggcatca gagcagattg
     1141 tactgagagt gcaccatagg ggatcgggag atctcccgat ccgtcgacgt caggtggcac
     1201 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     1261 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     1321 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     1381 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     1441 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     1501 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     1561 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     1621 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     1681 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     1741 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     1801 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     1861 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     1921 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     1981 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     2041 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     2101 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     2161 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     2221 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     2281 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     2341 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2401 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2461 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     2521 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2581 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2641 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2701 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     2761 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2821 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2881 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2941 aaacgccagc aacggagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata
     3001 tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa
     3061 ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc
     3121 ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac
     3181 aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag
     3241 cggtccaatg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg
     3301 atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc
     3361 ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgggg agctttttgc
     3421 aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg
     3481 cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atgagcttgg cccattgcat
     3541 acgttgtatc catatcataa tatgtacatt tatattggct catgtccaac attaccgcca
     3601 tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     3661 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     3721 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     3781 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     3841 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     3901 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     3961 gtattagtca tcgctattac catgcatggt gatgcggttt tggcagtaca tcaatgggcg
     4021 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     4081 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
     4141 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
     4201 gaagtcaagc ttaagtgacc agctacagtc ggaaaccatc agcaagaggt cattgttcac
     4261 catgggttaa cggatccatc gattctagag gtaccgctag cctcgagatc tgtcgggtag
     4321 cttatcagac tgatgttgac tgttgaatct catggcaaca ccagtcgatg ggctgtctga
     4381 caggatcctc gagtgacctt cagaccttgg cactggaggt ggcccggcag aagcgcggca
     4441 tcgtggatca gtgctgcacc agcatctgct ctctctacca actggagaac tactgcaact
     4501 aggcccacca ctaccctgtc cacccctctg caatgaataa aacctttgaa agagcactac
     4561 aagttgtgtg tacatgcgtg catgtgcata tgtggtgcgg ggggaacatg agtggggctg
     4621 gctggagtgg cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt
     4681 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     4741 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     4801 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     4861 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     4921 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     4981 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     5041 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     5101 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     5161 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     5221 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     5281 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     5341 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     5401 gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     5461 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     5521 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     5581 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     5641 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     5701 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     5761 tgatttaaaa cttcattttt aatttaaaag gatctaggct gctgcttgca aacaaaaaaa
     5821 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5881 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     5941 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6001 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6061 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6121 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6181 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6241 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6301 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6361 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6421 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     6481 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     6541 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     6601 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     6661 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     6721 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc
     6781 tagctagagg tcgacggtat acagacatga taagatacat tgatgagttt ggacaaacca
     6841 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     6901 ttgtaaccat tataagctgc aataaacaag ttggggtggg cgaagaactc cagcatgaga
     6961 tccccgcgct ggaggatcat ccagccggcg tcccggaaaa cgattccgaa gcccaacctt
     7021 tcatagaagg cggcggtgga atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg
     7081 gtcatttcga accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga
     7141 tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc
     7201 cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca
     7261 cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg
     7321 gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga
     7381 gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat
     7441 cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt
     7501 cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg
     7561 atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca
     7621 atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc
     7681 ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg cagttcattc agggcaccgg
     7741 acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg
     7801 catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag
     7861 cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg
     7921 tctcttgatc agatccgaaa atggatatac aagctcccgg gagctttttg caaaagccta
     7981 ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggcagag gcggcctcgg
     8041 cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg
     8101 gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat
     8161 gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg
     8221 actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac
     8281 accctaactg acacacattc cacagaatta attcgcgtta aatttttgtt aaatcagctc
     8341 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     8401 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     8461 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     8521 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     8581 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     8641 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     8701 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtgg ggataccccc tagagcccca
     8761 gctggttctt tccgcctcag aagccataga gcccaccgca tccccagcat gcctgctatt
     8821 gtcttcccaa tcctccccct tgctgtcctg ccccacccca ccccccagaa tagaatgaca
     8881 cctactcaga caatgcgatg caatttcctc attttattag gaaaggacag tgggagtggc
     8941 accttccagg gtcaaggaag gcacggggga ggggcaaaca acagatggct ggcaactaga
     9001 aggcacagtc gaggctgatc agcgagctct agcatttagg tgacactata gaatagggcc
     9061 cttaatgatg atg
//
