LOCUS       sequence-424688-        7388 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human LLGL2. C-terminal HiBiT 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7388)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 7388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-C_LLGL2:1-1020
FEATURES             Location/Qualifiers
     source          1..7388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    4451..4572
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4462..4481)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(4687..4720)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4730..4777
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4809..5603
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4863..4882)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5473..5492
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(5761..6349)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5841..5860)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    6475..6523
                     /note="synthetic polyadenylation signal"
     CDS             complement(6547..6945)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      6942..6951
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(6976..7333)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6990..7125
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        7142..7333
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     7290..7310
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggcga tcgcaacaag cttcgggaag
     1201 atctggagct agcggcgggg gatccggtgg gaattcgaca ccatgaggcg gttcctgagg
     1261 ccagggcatg accctgtgcg ggagaggctc aagcgggacc tgttccagtt taacaagacg
     1321 gtggagcatg gcttcccgca ccagcccagc gccctcggct acagcccgtc cctgcgcatc
     1381 ctggccatcg gcacccgttc tggagccatc aagctctacg gagccccagg cgtggagttc
     1441 atggggctgc accaggagaa caacgctgtg acgcagatcc acctcctgcc cggccagtgc
     1501 cagctggtca ccctgctgga tgacaacagc ctgcaccttt ggagcctgaa ggtcaagggc
     1561 ggggcatcgg agctgcagga ggatgagagc ttcacactgc gtggaccccc aggggctgcc
     1621 cccagtgcca cacagatcac cgtggtcctg ccacattcct cctgcgagct gctctacctg
     1681 ggcaccgaga gtggcaacgt gtttgtggtg cagctgccag cttttcgtgc gctggaggac
     1741 cggaccatca gctcggacgc ggtgctgcag cggttgccag aggaggcccg ccaccggcgt
     1801 gtgttcgaga tggtggaggc actgcaggag caccctcgag accccaacca gatcctgatc
     1861 ggctacagcc gaggcctcgt tgtcatctgg gacctacagg gcagccgcgt gctctaccac
     1921 ttcctcagca gccagcaact ggagaacatc tggtggcagc gggacggccg cctgctcgtc
     1981 agctgtcact ctgacggcag ctactgccag tggcccgtgt ccagcgaagc ccagcaacca
     2041 gagcccctcc gcagcctcgt gccttacggt ccctttcctt gcaaagcgat taccagaatc
     2101 ctctggctga ccactaggca ggggttgccc ttcaccatct tccagggtgg catgccacgg
     2161 gccagctacg gggaccgcca ctgcatctca gtgatccacg atggccagca gacggccttc
     2221 gacttcacct cccgtgtcat cggcttcact gtcctcacag aggcagaccc tgcagccacc
     2281 tttgacgacc cctatgccct ggtggtgctg gctgaggagg agctggtggt gattgacctg
     2341 cagacagcag gctggccacc ggtccagctg ccctacctgg cttctctgca ctgttccgcc
     2401 atcacctgct ctcaccacgt ctccaacatc ccgctgaagc tgtgggagcg gatcattgcc
     2461 gccggcagcc ggcagaacgc acacttctcc accatggagt ggccaattga tggtggcacc
     2521 agcctgaccc cagccccacc ccagagggac ctgctgctca cagggcacga ggacggcacg
     2581 gtgcggttct gggatgcctc gggtgtctgc ctgcggctgc tctacaaact cagcactgtg
     2641 cgcgtgttcc tcaccgacac ggaccccaac gagaacctca gtgcccaggg cgaggacgag
     2701 tggcccccac tccgcaaggt gggctccttt gacccctaca gtgatgaccc ccggctgggc
     2761 atccagaaga tcttcctctg caagtacagc ggctacctgg ctgtggcagg cacggcaggg
     2821 caggtgctgg tactggaact gaatgacgag gcagcggagc aggctgtgga gcaggtggag
     2881 gccgacctgc tgcaggacca agagggctac cgctggaagg ggcacgagcg cctggcagcc
     2941 cgctcagggc ccgtgcgctt tgagcctggc tttcagccct tcgtgttggt gcagtgtcag
     3001 cccccggctg tggtcacctc cttggccctg cactctgagt ggcggctcgt ggccttcggc
     3061 accagccatg gctttggcct ctttgaccac cagcagcggc ggcaggtctt tgttaagtgc
     3121 acactgcacc ccagtgacca gctggccttg gagggcccac tctcccgcgt caagtccctc
     3181 aagaagtcct tgcgtcagtc attccgccgg atgcgtcgga gccgggtgtc cagccggaag
     3241 cggcacccag ctggcccccc aggagaggca caggagggga gtgccaaggc tgagcggcca
     3301 ggcctccaga acatggagct ggcgcctgtg cagcgcaaga tcgaggctcg ctcggcagag
     3361 gactccttca caggcttcgt ccggaccctg tactttgctg acacctacct gaaggacagc
     3421 tcccggcact gcccctcgct gtgggctggc accaatgggg gcaccatcta tgccttctcc
     3481 ctgcgtgtgc ctcccgccga gcggagaatg gatgagtctg tgcgggcaga gcaggccaag
     3541 gagatccagc tgatgcaccg ggcgccggtg gtgggcatcc tggtgctcga cggacacagc
     3601 gtaccccttc ctgagcccct cgaagtggcc catgatctgt cgaagagccc tgacatgcag
     3661 ggaagccacc agctgctcgt cgtatcagag gagcagttca aggtgttcac gctgcccaag
     3721 gtgagtgcca agctgaagtt gaagctgacg gccctggagg gctcaagagt gcggcgggtc
     3781 agcgtggccc acttcggcag tcgtcgagcc gaggactacg gggagcacca cctggcagtc
     3841 cttaccaacc tgggcgacat ccaggtggtc tcactgcccc tgctcaagcc ccaggtgcgc
     3901 tacagctgca tccgccggga ggacgtcagt ggcatcgcct cctgcgtctt caccaaatat
     3961 ggccaaggct tctacctgat ctcaccctcg gagtttgagc gcttctctct ctccaccaag
     4021 tggctggtgg agccccggtg tctggtggat tcagcagaaa ccaagaacca ccgccctggt
     4081 aacggtgcgg gccccaagaa ggccccgagc cgagccagga actcagggac tcagagtgat
     4141 ggcgaggaga agcagcccgg cctggtgatg gagcgcgctc tgctcagtga tgagagagtc
     4201 ctgaaggaaa tccagagcac actggaggga gaccgcggga gcggcaactg gcgttcacat
     4261 cgagcggccg tggggtgcag cctcagcaat ggcggagcag agggctcgag cggtgggagc
     4321 tccggtgtga gcggctggcg gctgttcaag aagattagct aagtttaaac ggccgcgact
     4381 ctagactgca ggcatgcaag ctgatttggc tgctgccacc gctgagcaag gccgcttcga
     4441 gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     4501 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     4561 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg
     4621 tgggaggttt ttttaagcaa gtaaaacctc tacaaatgtg gtaaaatctt aattaagact
     4681 tctaatataa cttcgtatag catacattat acgaagttat ggttccactg ttgacaatta
     4741 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaacccagga
     4801 ggcagatcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     4861 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     4921 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     4981 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     5041 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     5101 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     5161 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     5221 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     5281 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     5341 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     5401 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     5461 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     5521 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     5581 gccttcttga cgagttcttc tgattcgaaa tgaccgacca agcgacgccc aaccggtatc
     5641 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     5701 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     5761 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     5821 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     5881 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     5941 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     6001 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     6061 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     6121 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     6181 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     6241 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     6301 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatttcaag aagatccttt
     6361 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     6421 catgagatta tcaaaaagga tcttcaccta gatcctttta tagtccggaa tcgtcacaca
     6481 aaaaaccaac acacagatgt aatgaaaata aagatatttt attgcggcca tcgtgatggc
     6541 tagagtttag ccctcccaca cataaccaga gggcagcaat tcacgaatcc caactgccgt
     6601 cggctgtcca tcactgtcct tcactatggc tttgatccca ggatgcagat cgagaagcac
     6661 ctgtcggcac cgtccgcagg ggctcaagat gcccctgttc tcatttccga tcgcgacgat
     6721 acaagtcagg ttgccagctg ccgcagcagc agcagtgccc agcaccacga gttctgcaca
     6781 aggtccccca gtaaaatgat atacattgac accagtgaag atgcggccgt cgctagagag
     6841 agctgcgctg gcgacgctgt agtcttcaga gatggggatg ctgttgattg tagccgttgc
     6901 tctttcaatg agggtggatt cttcttgaga caaaggcttg gccatggtgg cgctagtgtc
     6961 agaagaatcg agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg
     7021 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
     7081 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
     7141 cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     7201 gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct
     7261 gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc acagctggtt
     7321 ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt caggccatgg
     7381 tgctgcgc
//
