LOCUS       sequence-42469-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   He A, Kong SW, Ma Q, Pu WT
  TITLE     Co-occupancy by multiple cardiac transcription factors identifies 
            transcriptional enhancers active in heart.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Apr 5. 108(14):5632-7.
  PUBMED    21415370
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2011 Apr 5. 108(14):5632-7."
COMMENT     Sequence Label: pEntr-GATA4flbio
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    86..185
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     185..201
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     185..201
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(219..263)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             complement(276..299)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tttattgttc atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac
       61 acgggccaga gctgcagctg gatggcaaat aatgatttta ttttgactga tagtgacctg
      121 ttcgttgcaa caaattgata agcaatgctt tcttataatg ccaactttgt acaagaaagc
      181 tgggtctaga actagtggat cctcgagctc ggcgcgcctt cgtgccattc gattttctga
      241 gcctcgaaga tgtcgttcag gccggaccca tcacctttat cgtcatcgtc tttgtagtca
      301 gatctcgcgg tgattatgtc cccatgactg tcagccagga ccaggctgtt ccaagagtcc
      361 tgcttggagc tggcctgcga tgtctgagtg acaggagatg catagccttg tggggacagc
      421 ttcagagcag acagcactgg atggatggag ggcccgtggc cggacacagt actgaatgtc
      481 tgggacatgg agctgctgtg cccatagtga gatgacagcc cgggctctgt cttgatgggg
      541 cgcatctctt cactgctgct gctgctgcta gtggcattgc tggagttacc gctggaggca
      601 ccactggagg gagggagggt ctcaccagca ggacctgctg gcgtcttaga tttattcagg
      661 ttcttgggct tccgttttct ggtttgaatc ccctccttcc gcattgcaag aggcctggga
      721 accccatgga gcttcatgta gaggccgcag gcattacata caggctcacc ctcggcatta
      781 cgacgccaca gcgtggtggt ggtagtctgg cagttggcac aggagaggcc tacccggcgg
      841 g
//
