LOCUS       sequence-42471-e         666 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 666)
  AUTHORS   He A, Kong SW, Ma Q, Pu WT
  TITLE     Co-occupancy by multiple cardiac transcription factors identifies 
            transcriptional enhancers active in heart.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Apr 5. 108(14):5632-7.
  PUBMED    21415370
REFERENCE   2  (bases 1 to 666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2011 Apr 5. 108(14):5632-7."
COMMENT     Sequence Label: pEntr-Tbx5flbio
FEATURES             Location/Qualifiers
     source          1..666
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    76..175
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(220..264)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             complement(277..300)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac acgggccaga
       61 gctgcagctg gatggcaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa
      121 caaattgata agcaatgctt tcttataatg ccaactttgt acaagaaagc tgggtctaga
      181 tatctcgagt gcggccgcct acctcgagct cggcgcgcct tcgtgccatt cgattttctg
      241 agcctcgaag atgtcgttca ggccggaccc atcaccttta tcgtcatcgt ctttgtagtc
      301 acgcgtgcta ttgtcgctcc actctggcac catgccaact ccgtgcacag agtggtactg
      361 atgaggggat agagtccttg gcacgccatg agagtagagg aactcagggg gctgaagggt
      421 gccaggggac tgcaggccag tctgaggccc acactgcctg accacaggct ggtgggccac
      481 ggaggtctgg tgctggaaca ttccctctcc cagctgtggg gagccatggt tggccatgcc
      541 agccagccga gggaccaggg gccccgaggt gaagtgagcg gagaagtgct ggtagggtag
      601 cctgtccatg ggctgcacgg tggtgacggt gcagctgctg taggaaggca tgcttggcca
      661 cgtgtt
//
