LOCUS       sequence-424715-        3159 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pDONR223 SARS-CoV-1 ORF7a_stop
FEATURES             Location/Qualifiers
     source          1..3159
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(176..275)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(291..308)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(291..307)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(300..322)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      371..457
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      549..576
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(735..752)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(906..1494)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(986..1005)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1587..2378)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     2809..2825
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2809..2825
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2830..2849
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2830..2848
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2866..2965
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gtgcaagtta gtgcaaattt attgtcagca agagggtgaa atggtgaatt gccctcgtat
       61 gttcctgatg ggcaaggttc ttttagtagt acagtcgtac ctctaacaca ctcctgatag
      121 tgatatagct cgcaagatgt aaatacaatc aatgtcagga agagaataat tttcatgcca
      181 acttttttgt acaaagttgg cattataaaa aagcattgct catcaatttg ttgcaacgaa
      241 caggtcacta tcagtcaaaa taaaatcatt atttggggcc cgagcttaag actggccgtc
      301 gttttacaac gtcgtgactg ggaaaacatc catgctagcg ttaacgcgag agtagggaac
      361 tgccaggcat caaataaaac gaaaggctca gtcggaagac tgggcctttc gttttatctg
      421 ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt
      481 tgtgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca
      541 aactaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctcttcctgg
      601 ctagcggtac gcgtattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
      661 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
      721 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
      781 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
      841 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      901 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      961 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     1021 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     1081 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     1141 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     1201 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     1261 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     1321 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     1381 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     1441 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     1501 cctttgatct tttctacggg gtctgacgct cagtggaacg acgcgtaact cacgttaagg
     1561 gattttggtc atgggtggct cgagggttat ttgccgacta ccttggtgat ctcgcctttc
     1621 acgtagtgga caaattcttc caactgatct gcgcgcgagg ccaagcgatc ttcttcttgt
     1681 ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg caggcgctcc
     1741 attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc gctgtaccaa
     1801 atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg cggcgagttc
     1861 catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac cggatcaaag
     1921 agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt tgtcagcaag
     1981 atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat gtcattgcgc
     2041 tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat gatgtcgtcg
     2101 tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg ggaagccgaa
     2161 gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct tacggtcacc
     2221 gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc ggagccgtac
     2281 aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac tacctctgat
     2341 agttgagtcg atacttcggc gatcaccgct tccctcataa tgtttaactt tgttttaggg
     2401 cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg
     2461 taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa acagtcataa
     2521 caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac
     2581 cagttgcgtg agcgcatacg ctacttgcat tacagcttac gaaccgaaca ggcttatgtc
     2641 cactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggtagc
     2701 agcgaagtcg aggcatttct gtcctggctg gtctagagga ggcatgatga tatattttta
     2761 tcttgtgcaa tgtaacatca gagattttga gacacgggcc agagctgcca ggaaacagct
     2821 atgaccatgt aatacgactc actatagggg atatcagctg gatggcaaat aatgatttta
     2881 ttttgactga tagtgacctg ttcgttgcaa caaattgata agcaatgctt tcttataatg
     2941 ccaactttgt acaagaaagt tgggtatcat tctgtctttc tcttaatggt gaagcaaagt
     3001 attaaaaata ctagagcagc aacaatgaga aaaagtggcg agtagagctc ttgttgaacc
     3061 tcctcttgtc tgatgaaaag ttttggtgaa actgatcttg cacgcagctg ataggtatgt
     3121 cgagtaccgt cagcacaagc aaaagcaaag tgtgtgcta
//
