LOCUS       sequence-42472-e         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Nowotschin S, Eakin GS, Hadjantonakis AK
  TITLE     Dual transgene strategy for live visualization of chromatin and 
            plasma membrane dynamics in murine embryonic stem cells and 
            embryonic tissues.
  JOURNAL   Genesis. 2009 May;47(5):330-6.
  PUBMED    19358158
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis."; 
            date: "2009-05"; volume: "47(5)"; pages: "330-6"
COMMENT     Sequence Label: pCAG:myr-mRFP1
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(120..143)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(318..337)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     588..606
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(740..760)
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     regulatory      757..766
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttggccacac cagccaccac cttctgatag gcagcctgca cctgaggagt gaattcttat
       61 tgcgccggtg gagtggcggc cctcggcgcg ctcgtactgt tccacgatgg tgtagtcctc
      121 gttgtgggag gtgatgtcca gcttgatgtc ggtcttgtag gcgccgggca gctgcacggg
      181 cttcttggcc atgtaggtgg tcttgacctc ggcgtcgtag tggccgccgt ccttcagctt
      241 cagcctcatc ttgatctcgc ccttcagggc gccgtcctcg gggtacatcc gctcggtgga
      301 ggcctcccag cccatggtct tcttctgcat tacggggccg tcggagggga agttggtgcc
      361 gcgcagcttc accttgtaga tgaactcgcc gtcctgcagg gaggagtcct gggtcacggt
      421 caccacgccg ccgtcctcga agttcatcac gcgctcccac ttgaagccct cggggaaagg
      481 acagcttcaa gtagtcgggg atgtcggcgg ggtgcttcac gtaggccttg gagccgtact
      541 ggaactgagg ggacaggatg tcccaggcga agggcagggg gccgcccttg gtcaccttca
      601 gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc ctcgccctcg atctcgaact
      661 cgtggccgtt cacggagccc tccatgcgca ccttgaagcg catgaactcc ttgatgacgt
      721 cctcggagga ggcctttggc ttgctcttgc tgcttcccat ggtggcgaat tctttgccaa
      781 aatgatgaga cagcacaata accag
//
