LOCUS       sequence-424732-        5126 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5126)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 5126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-H3-23
FEATURES             Location/Qualifiers
     source          1..5126
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        57..414
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        57..248
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(80..100)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      265..400
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             449..1243
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             449..1243
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(503..522)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1113..1132
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1431..1450)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1475..1522
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1851..2439
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2340..2359
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2614..2917
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2918..3121
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3072..3092
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3151..3160
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3568..3620
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      3628..3637
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3634..4344
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3779..3797)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4047..4066
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    4467..4588
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4504..4523)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4558..4577
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4595..5050)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4732..4753)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4944..4963
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        join(5077..5126,1..55)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga
       61 ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
      121 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
      181 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
      241 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
      301 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
      361 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga
      421 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
      481 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
      541 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
      601 cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc
      661 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
      721 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
      781 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
      841 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
      901 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
      961 cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     1021 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     1081 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     1141 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     1201 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     1261 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     1321 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     1381 cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg
     1441 aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg
     1501 cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
     1561 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc
     1621 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct
     1681 gccatagcct caggttactc atatatactt tagattgatt taaaacttca tttttaattt
     1741 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     1801 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     1861 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     1921 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     1981 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     2041 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     2101 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     2161 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     2221 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     2281 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     2341 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     2401 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     2461 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     2521 gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat caattacggg
     2581 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     2641 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     2701 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     2761 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     2821 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     2881 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     2941 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     3001 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     3061 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     3121 tggtttagtg aaccgtcaga tccgctagcc gccaccatgg ctcgtactaa acagacagct
     3181 cggaaatcca ccggcggtaa agcgccacgc aagcagctgg ctaccaaggc tgctcgcaag
     3241 agcgcgccgg ctaccggcgg cgtgaaaaag cctcaccgtt accgcccggg cactgtggct
     3301 ctgcgcgaga tccgccgcta ccaaaagtcg accgagttgc tgattcggaa gctgccgttc
     3361 cagcgcctgg tgcgagaaat cgcccaagac ttcaagaccg atcttcgctt ccagagctct
     3421 gcggtgatgg cgctgcagga ggcttgtgag gcctacttgg tagggctctt tgaggacaca
     3481 aacctttgcg ccatccatgc taagcgagtg actattatgc ccaaagacat ccagctcgct
     3541 cgccgcattc gcggagaaag agcggatctc gagctcaagc ttcgaattct gcagtcgacg
     3601 gtaccgcggg cccgggatcc accggtcgcc accatggtga gcaagggcga ggaggataac
     3661 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
     3721 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     3781 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
     3841 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
     3901 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     3961 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     4021 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     4081 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     4141 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     4201 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     4261 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     4321 ggcatggacg agctgtacaa gtaaagcggc cgcgactcta gatcataatc agccatacca
     4381 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     4441 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     4501 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     4561 gtttgtccaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa tattttgtta
     4621 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     4681 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     4741 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     4801 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     4861 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     4921 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     4981 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     5041 cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     5101 ttctaaatac attcaaatat gtatcc
//
