LOCUS       sequence-424737-       14131 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A lentiviral vector expressing the ABE8e-SpRY base editor and an 
            sgRNA cloning site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14131)
  AUTHORS   Ryu J, Barkal S, Yu T, Jankowiak M, Zhou Y, Francoeur M, Phan QV, Li
            Z, Tognon M, Brown L, Love MI, Bhat V, Lettre G, Ascher DB, Cassa 
            CA, Sherwood RI, Pinello L
  TITLE     Joint genotypic and phenotypic outcome modeling improves base 
            editing variant effect quantification.
  JOURNAL   Nat Genet. 2024 Apr 24. doi: 10.1038/s41588-024-01726-6.
  PUBMED    38658794
REFERENCE   2  (bases 1 to 14131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2024 Apr 24. doi: 10.1038/s41588-024-01726-6."
COMMENT     Sequence Label: lentiCRISPRv2FE-ABE8e-SpRY
FEATURES             Location/Qualifiers
     source          1..14131
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..199
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     154..174
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             217..397
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    444..569
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1062..1295
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1480..1524
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1822..1939
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1990..2238
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1990..2010
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2161..2180
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        2782..2993
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             3049..3069
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3664..7764
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAERTRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFLWPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAKQLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTRLGAPRAFKYFDTTIDPKQYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7819..7839
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7852..7917
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             7936..7992
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7993..8589
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     8486..8506
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8605..9193
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8658..8678)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9076..9087)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9265..9498
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9524..9541)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9530..9754
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9800..10228
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9887..9906)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10097..10118
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10237..10257)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10242..10571
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10422..10557
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10484..10503
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10619..10666
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10685..11059
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11189..11310
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11226..11245)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11280..11299
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11359..11375)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11359..11375)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11372..11394)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11383..11399
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11407..11413,11414..11431,11432..11437))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11452..11473
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11590..11607)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11761..12349)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11841..11860)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12520..13311,13312..13380))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13143..13162
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13381..13485)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(13560..13579)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        13751..14130
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
       61 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      181 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      361 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      421 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      481 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      541 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      601 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      661 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      721 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      781 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      841 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      901 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      961 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1021 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1081 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1141 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1201 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1261 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1321 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1381 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1441 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1501 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1561 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1621 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1681 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1741 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     1801 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     1861 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1921 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaat
     1981 taaggtaccg agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct
     2041 gttagagaga taattagaat taatttgact gtaaacacaa agatattagt acaaaatacg
     2101 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
     2161 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
     2221 tggaaaggac gaaacaccgg agacgttaac tccatatttt tcatgagcga ttctatcacc
     2281 caaatcactt gttacaaaac ttgaatatag agccggattt tttggaatgc tatttaagcg
     2341 ttttagattt gataattttc aatcaattaa aattggtcct gtttgatttc catctaatct
     2401 tacaaaatga ccatcatgtt ttattgccat tccaaatctg tcagcatctg ggtcattcat
     2461 aataataata tctgcatcat gtttaatacc atattcaagc ggtatttttc atgcaggatc
     2521 aaattctgga tttggattta caacattttt aaatgtttca tcttcaaatg catcgtctct
     2581 gtttaagagc tatgctggaa acagcatagc aagtttaaat aaggctagtc cgttatcaac
     2641 ttgaaaaagt ggcaccgagt cggtgctttt ttgttttgta catgcaaaga tggataaagt
     2701 tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa
     2761 ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2821 ggggaggggt cggcaattga tccggtgcct agagaaggtg gcgcggggta aactgggaaa
     2881 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2941 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggaccggt
     3001 tctagagcca ccatgaaacg gacagccgac ggaagcgagt tcgagtcacc aaagaagaag
     3061 cggaaagtct ctgaggtgga gttttcccac gagtactgga tgagacatgc cctgaccctg
     3121 gccaagaggg cacgcgatga gagggaggtg cctgtgggag ccgtgctggt gctgaacaat
     3181 agagtgatcg gcgagggctg gaacagagcc atcggcctgc acgacccaac agcccatgcc
     3241 gaaattatgg ccctgagaca gggcggcctg gtcatgcaga actacagact gattgacgcc
     3301 accctgtacg tgacattcga gccttgcgtg atgtgcgccg gcgccatgat ccactctagg
     3361 atcggccgcg tggtgtttgg cgtgaggaac agcaaaagag gcgccgcagg ctccctgatg
     3421 aacgtgctga actaccccgg catgaatcac cgcgtcgaaa ttaccgaggg aatcctggca
     3481 gatgaatgtg ccgccctgct gtgcgatttc taccggatgc ctagacaggt gttcaatgct
     3541 cagaagaagg cccagagctc catcaactcc ggaggatcta gcggaggctc ctctggctct
     3601 gagacacctg gcacaagcga gagcgcaaca cctgaaagca gcgggggcag cagcgggggg
     3661 tcagacaaga agtacagcat cggcctggcc atcggcacca actctgtggg ctgggccgtg
     3721 atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa caccgaccgg
     3781 cacagcatca agaagaacct gatcggagcc ctgctgttcg acagcggcga aacagccgag
     3841 agaacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa ccggatctgc
     3901 tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt cttccacaga
     3961 ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc catcttcggc
     4021 aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca cctgagaaag
     4081 aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctggc cctggcccac
     4141 atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga caacagcgac
     4201 gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga ggaaaacccc
     4261 atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg ccagactgag caagagcaga
     4321 cggctggaaa atctgatcgc ccagctgccc ggcgagaaga agaatggcct gttcggaaac
     4381 ctgattgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga cctggccgag
     4441 gatgccaaac tgcagctgag caaggacacc tacgacgacg acctggacaa cctgctggcc
     4501 cagatcggcg accagtacgc cgacctgttt ctggccgcca agaacctgtc cgacgccatc
     4561 ctgctgagcg acatcctgag agtgaacacc gagatcacca aggcccccct gagcgcctct
     4621 atgatcaaga gatacgacga gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg
     4681 cagcagctgc ctgagaagta caaagagatt ttcttcgacc agagcaagaa cggctacgcc
     4741 ggctacattg acggcggagc cagccaggaa gagttctaca agttcatcaa gcccatcctg
     4801 gaaaagatgg acggcaccga ggaactgctc gtgaagctga acagagagga cctgctgcgg
     4861 aagcagcgga ccttcgacaa cggcagcatc ccccaccaga tccacctggg agagctgcac
     4921 gccattctgc ggcggcagga agatttttac ccattcctga aggacaaccg ggaaaagatc
     4981 gagaagatcc tgaccttccg catcccctac tacgtgggcc ctctggccag gggaaacagc
     5041 agattcgcct ggatgaccag aaagagcgag gaaaccatca ccccctggaa cttcgaggaa
     5101 gtggtggaca agggcgcttc cgcccagagc ttcatcgagc ggatgaccaa cttcgataag
     5161 aacctgccca acgagaaggt gctgcccaag cacagcctgc tgtacgagta cttcaccgtg
     5221 tataacgagc tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc cgccttcctg
     5281 agcggcgagc agaaaaaggc catcgtggac ctgctgttca agaccaaccg gaaagtgacc
     5341 gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc
     5401 tccggcgtgg aagatcggtt caacgcctcc ctgggcacat accacgatct gctgaaaatt
     5461 atcaaggaca aggacttcct ggacaatgag gaaaacgagg acattctgga agatatcgtg
     5521 ctgaccctga cactgtttga ggacagagag atgatcgagg aacggctgaa aacctatgcc
     5581 cacctgttcg acgacaaagt gatgaagcag ctgaagcggc ggagatacac cggctggggc
     5641 aggctgagcc ggaagctgat caacggcatc cgggacaagc agtccggcaa gacaatcctg
     5701 gatttcctga agtccgacgg cttcgccaac agaaacttca tgcagctgat ccacgacgac
     5761 agcctgacct ttaaagagga catccagaaa gcccaggtgt ccggccaggg cgatagcctg
     5821 cacgagcaca ttgccaatct ggccggcagc cccgccatta agaagggcat cctgcagaca
     5881 gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc acaagcccga gaacatcgtg
     5941 atcgaaatgg ccagagagaa ccagaccacc cagaagggac agaagaacag ccgcgagaga
     6001 atgaagcgga tcgaagaggg catcaaagag ctgggcagcc agatcctgaa agaacacccc
     6061 gtggaaaaca cccagctgca gaacgagaag ctgtacctgt actacctgca gaatgggcgg
     6121 gatatgtacg tggaccagga actggacatc aaccggctgt ccgactacga tgtggaccat
     6181 atcgtgcctc agagctttct gaaggacgac tccatcgaca acaaggtgct gaccagaagc
     6241 gacaagaacc ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag
     6301 aactactggc ggcagctgct gaacgccaag ctgattaccc agagaaagtt cgacaatctg
     6361 accaaggccg agagaggcgg cctgagcgaa ctggataagg ccggcttcat caagagacag
     6421 ctggtggaaa cccggcagat cacaaagcac gtggcacaga tcctggactc ccggatgaac
     6481 actaagtacg acgagaatga caagctgatc cgggaagtga aagtgatcac cctgaagtcc
     6541 aagctggtgt ccgatttccg gaaggatttc cagttttaca aagtgcgcga gatcaacaac
     6601 taccaccacg cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag
     6661 taccctaagc tggaaagcga gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag
     6721 atgatcgcca agagcgagca ggaaatcggc aaggctaccg ccaagtactt cttctacagc
     6781 aacatcatga actttttcaa gaccgagatt accctggcca acggcgagat ccggaagcgg
     6841 cctctgatcg agacaaacgg cgaaaccggg gagatcgtgt gggataaggg ccgggatttt
     6901 gccaccgtgc ggaaagtgct gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg
     6961 cagacaggcg gcttcagcaa agagtctatc agacccaaga ggaacagcga taagctgatc
     7021 gccagaaaga aggactggga ccctaagaag tacggcggct tcctgtggcc caccgtggcc
     7081 tattctgtgc tggtggtggc caaagtggaa aagggcaagt ccaagaaact gaagagtgtg
     7141 aaagagctgc tggggatcac catcatggaa agaagcagct tcgagaagaa tcccatcgac
     7201 tttctggaag ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag
     7261 tactccctgt tcgagctgga aaacggccgg aagagaatgc tggcctctgc caagcagctg
     7321 cagaagggaa acgaactggc cctgccctcc aaatatgtga acttcctgta cctggccagc
     7381 cactatgaga agctgaaggg ctcccccgag gataatgagc agaaacagct gtttgtggaa
     7441 cagcacaagc actacctgga cgagatcatc gagcagatca gcgagttctc caagagagtg
     7501 atcctggccg acgctaatct ggacaaagtg ctgtccgcct acaacaagca ccgggataag
     7561 cccatcagag agcaggccga gaatatcatc cacctgttta ccctgaccag actgggagcc
     7621 cctagagcct tcaagtactt tgacaccacc atcgacccca agcagtacag aagcaccaaa
     7681 gaggtgctgg acgccaccct gatccaccag agcatcaccg gcctgtacga gacacggatc
     7741 gacctgtctc agctgggagg cgacggatcc ggcggaggcg gaagcgggaa aagaaccgcc
     7801 gacggcagcg aattcgagcc caagaagaag aggaaagtct cgagcggagg cgactacaaa
     7861 gaccatgacg gtgattataa agatcatgac atcgattaca aggatgacga tgacaaggga
     7921 ggcggtggaa gcggcgcaac aaacttctct ctgctgaaac aagccggaga tgtcgaagag
     7981 aatcctggac cgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     8041 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     8101 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     8161 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     8221 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     8281 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     8341 cccaaggagc ccgcgtggtt cctggccacc gtcggagtct cgcccgacca ccagggcaag
     8401 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     8461 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     8521 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     8581 ggtgcctgaa cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     8641 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     8701 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     8761 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     8821 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     8881 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     8941 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     9001 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     9061 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     9121 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     9181 gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     9241 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     9301 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     9361 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     9421 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     9481 tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     9541 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     9601 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     9661 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     9721 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     9781 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     9841 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     9901 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     9961 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
    10021 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
    10081 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
    10141 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
    10201 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
    10261 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
    10321 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
    10381 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
    10441 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
    10501 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
    10561 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt
    10621 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta
    10681 aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc
    10741 ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc
    10801 cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc
    10861 ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc
    10921 ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga
    10981 gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt
    11041 ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga
    11101 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
    11161 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
    11221 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    11281 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
    11341 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
    11401 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
    11461 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
    11521 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
    11581 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
    11641 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    11701 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    11761 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    11821 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    11881 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    11941 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    12001 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    12061 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    12121 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    12181 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
    12241 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    12301 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    12361 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    12421 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    12481 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    12541 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    12601 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    12661 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    12721 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    12781 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    12841 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    12901 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    12961 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    13021 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    13081 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    13141 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    13201 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    13261 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    13321 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    13381 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    13441 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    13501 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
    13561 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
    13621 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
    13681 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
    13741 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
    13801 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
    13861 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
    13921 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
    13981 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
    14041 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
    14101 atctacgtat tagtcatcgc tattaccatg g
//
