LOCUS       sequence-424738-       10133 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  18xGCN4pep transgene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10133)
  TITLE     Cheeseman lab plasmids Nov 2023
REFERENCE   2  (bases 1 to 10133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGS383_tdTomato-18xGCN4-T2A-BSD Lenti
FEATURES             Location/Qualifiers
     source          1..10133
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    96..117
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(132..137,138..155,156..162)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    170..186
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     175..197
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     194..210
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     194..210
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     229..249
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        231..249
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        277..503
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             504..684
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    731..856
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1353..1586
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1771..1815
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     1993..2013
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    2214..2331
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2403..2614
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2641..2650
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2647..4074
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             4120..4140
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4153..4197
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             4234..4299
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4315..4380
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4396..4461
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4477..4542
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4558..4623
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4639..4704
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4720..4785
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4801..4866
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4882..4947
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4963..5028
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             5044..5109
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             5125..5190
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     primer_bind     5206..5228
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     primer_bind     5229..5251
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             5254..5319
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             5335..5400
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             5416..5481
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             5497..5562
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             5578..5643
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             5659..5724
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             5758..5811
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5818..6216
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    6262..6850
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6315..6335)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6733..6744)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6922..7155
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7233..7354
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7270..7289)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7324..7343
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7394..7529
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7549..7568)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7550..7568)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7578..7595)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7578..7594)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7587..7609)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7736..8191
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7823..7842)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8033..8054
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8217..8321
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8322..8390,8391..9182)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8540..8559)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9353..9941
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9842..9861
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10095..10112
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
       61 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
      121 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
      181 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
      241 cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt
      301 agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc
      361 gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga
      421 cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta
      481 agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc
      541 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      601 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      661 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
      721 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
      781 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
      841 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
      901 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
      961 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     1021 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     1081 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     1141 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg
     1201 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     1261 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     1321 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     1381 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     1441 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     1501 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     1561 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     1621 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     1681 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     1741 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     1801 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     1861 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     1921 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     1981 aggggaccca gagagggcct atttcccatg attccttcat atttgcatat acgatacaag
     2041 gctgttagag agataattag aattaatttg actgtaaaca caaagatatt agtacaaaat
     2101 acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa
     2161 atggactatc ataaattcta gatcttgaga caaatggcag tattcatcca caattttaaa
     2221 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     2281 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     2341 tacagggaca gcagagatcc actttggcgc cggctcgtcg agtggctccg gtgcccgtca
     2401 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     2461 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     2521 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     2581 tctttttcgc aacgggtttg ccgccagaac acaggtgtcg tgacgcggga tccgatatcc
     2641 gccaccatgg tgagcaaggg cgaggaggtc atcaaagagt tcatgcgctt caaggtgcgc
     2701 atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     2761 tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc
     2821 tgggacatcc tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc
     2881 gacatccccg attacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     2941 aacttcgagg acggcggtct ggtgaccgtg acccaggact cctccctgca ggacggcacg
     3001 ctgatctaca aggtgaagat gcgcggcacc aacttccccc ccgacggccc cgtaatgcag
     3061 aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg
     3121 aagggcgaga tccaccaggc cctgaagctg aaggacggcg gccactacct ggtggagttc
     3181 aagaccatct acatggccaa gaagcccgtg caactgcccg gctactacta cgtggacacc
     3241 aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgctcc
     3301 gagggccgcc accacctgtt cctggggcat ggcaccggca gcaccggcag cggcagctcc
     3361 ggcaccgcct cctccgagga caacaacatg gccgtcatca aagagttcat gcgcttcaag
     3421 gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc
     3481 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc
     3541 ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac
     3601 cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc
     3661 gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac
     3721 ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta
     3781 atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc
     3841 gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg
     3901 gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg
     3961 gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag
     4021 cgctccgagg gccgccacca cctgttcctg tacggcatgg acgagctgta caagaccgga
     4081 ggggatcggt ggagctccac cggtggcggc cgctctagag agaatcttta ttttcagggc
     4141 gccgccaaat tcaaagaaac cgctgctgct aaattcgaac gccagcacat ggacagcgga
     4201 ggtggaggta ctagtctcga gggttctgga ggattgttgc ccaaaaacta ccacttggag
     4261 aacgaggtcg caaggttgaa aaaattggtc ggtgagcgtg gcggctcagg cggccttctt
     4321 ccaaaaaatt atcatcttga aaatgaagtt gctagactta aaaaacttgt tggagaaaga
     4381 ggcgggagcg gcggcctgct gcccaagaac taccacctgg agaacgaggt cgcccgcctg
     4441 aagaagctgg tcggcgagcg cggtggttct ggaggtttgc tcccaaagaa ctaccacttg
     4501 gaaaacgaag tcgcaagatt gaagaagttg gtcggtgaaa gaggaggaag cggaggatta
     4561 ttacctaaaa attatcattt ggaaaatgaa gttgctagat tgaagaagtt ggttggagag
     4621 agaggggggt ctggaggact actaccgaaa aactaccacc tagagaacga ggtggcccga
     4681 ctaaaaaagc tagtgggcga gcgcggcggc agtggcggcc tcctccccaa gaattaccac
     4741 ctcgaaaatg aagtagcaag gctcaagaag ctcgtaggag aaagaggtgg ttccggggga
     4801 ctccttccaa aaaactatca tcttgaaaac gaagttgcac gtcttaaaaa acttgttggc
     4861 gaacgtggcg gctctggcgg gctgctgccc aagaattacc acctggaaaa tgaagtggcc
     4921 cgcctgaaga agctggtggg cgaacgcgga ggcagtggag gcctactacc gaagaactac
     4981 cacctagaga acgaggtggc acgcctaaag aagctagtgg gcgagcgcgg cggctcgggt
     5041 ggtttattac ctaagaatta tcatttagaa aatgaagtag ctcgattaaa gaagttagta
     5101 ggtgaacgtg gtgggtctgg tggtcttctt ccgaagaact accaccttga gaacgaggta
     5161 gctcgtctta agaaacttgt aggtgagcgt ggtggaagcg gtggtgggct ggcaagccac
     5221 gtttggtgcc gggagctgca tgtgtcagag gaacttttgc caaagaatta tcatcttgag
     5281 aacgaagtgg ctcgtcttaa gaaactcgta ggagaaagag gtggcagtgg aggtctgctt
     5341 cccaagaatt accacctgga aaatgaggta gctagactga aaaagctcgt aggcgagcgc
     5401 gggggaagtg ggggattgct gccaaaaaat tatcatttgg agaacgaagt agcacgacta
     5461 aagaaattag ttggggagag ggggggatcg ggtggcttac tcccgaaaaa ttatcatctc
     5521 gaaaacgaag tggctcggct aaaaaagtta gttggagagc gtggcggttc tggaggccta
     5581 ttaccaaaaa actaccacct cgaaaatgag gtggcacgct taaaaaagct ggtcggagaa
     5641 cggggaggca gtggtggcct actacccaag aattatcatc ttgagaacga ggtagcgcgt
     5701 ttgaagaagc tcgtcggtga aagaggtggc tcaggagggc tgctcccaaa ggaattcgag
     5761 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc tatcgatatg
     5821 gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac
     5881 agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag cgacggccgc
     5941 atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga actcgtggtg
     6001 ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat
     6061 gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct cgatctgcat
     6121 cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt
     6181 gaattgctgc cctctggtta tgtgtgggag ggctaaggta ccactagtcc cgggatgcat
     6241 agcgctacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     6301 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     6361 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     6421 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     6481 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     6541 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     6601 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     6661 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     6721 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     6781 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     6841 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
     6901 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     6961 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7021 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7081 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7141 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
     7201 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     7261 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     7321 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     7381 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     7441 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     7501 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     7561 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     7621 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     7681 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     7741 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     7801 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     7861 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     7921 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     7981 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     8041 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     8101 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     8161 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     8221 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     8281 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     8341 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     8401 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     8461 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     8521 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     8581 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     8641 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     8701 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     8761 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     8821 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     8881 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     8941 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     9001 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     9061 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     9121 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     9181 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     9241 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     9301 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     9361 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     9421 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     9481 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     9541 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     9601 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     9661 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     9721 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     9781 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     9841 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     9901 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     9961 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    10021 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    10081 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca ata
//
