LOCUS       sequence-42474-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Nowotschin S, Hadjantonakis AK
  TITLE     Use of KikGR a photoconvertible green-to-red fluorescent protein for
            cell labeling and lineage analysis in ES cells and mouse embryos.
  JOURNAL   BMC Dev Biol. 2009 Sep 9;9:49.
  PUBMED    19740427
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Dev 
            Biol."; date: "2009-09-9"; volume: "9"; pages: "49"
COMMENT     Sequence Label: pCAG:KikGR
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(52..738)
                     /codon_start=1
                     /product="photoconvertible Kikume green-to-red fluorescent 
                     protein (Tsutsui et al., 2005)"
                     /label=KikGR1
                     /translation="MVSVITSEMKIELRMEGAVNGHKFVITGKGSGQPFEGIQNVDLTV
                     IEGGPLPFAFDILTTAFHYGNRVFVEYPEEIVDYFKQSFPEGYSWERSMSYEDGGICLA
                     TNNITMKKDGSNCFVNEIRFDGVNFPANGPVMQRKTVKWEPSTEKMYVRDGVLKGDVNM
                     ALLLQGGGHYRCDFRTTYKAKKVVQLPDYHFVDHQMEITSHDKDYNKVKLYEHAKAHSG
                     LPRLAK"
     primer_bind     complement(769..788)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(850..872)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 accagccacc accttctgat aggcagcctg cacctgagga gtgaattccg gttacttggc
       61 cagccttggc agcccggaat gagctttagc gtgctcatac agcttaacct tgttgtaatc
      121 cttgtcatgg cttgttatct ccatttgatg atccacgaag tgatagtctg gcaactggac
      181 aaccttcttt gctttgtaag tagttctgaa gtcacatcgg taatggccac ctccttgaag
      241 caacagagcc atgtttacat cacccttcag cactccatca cgcacataca ttttctcagt
      301 ggatggctcc catttgacgg tcttcctctg cataactgga ccattggcag gaaagttcac
      361 accatcaaat cgaatttcat tgacaaaaca gttgctgccg tctttcttca tcgttatatt
      421 gtttgtggcg aggcaaattc ccccgtcttc gtaactcatg cttcgttccc aagaataacc
      481 ctcaggaaac gactgcttga agtagtctac tatttcttct gggtattcga caaatacccg
      541 gttgccgtaa tggaatgctg ttgtcaggat atcgaaagca aaaggaagag gtccgccctc
      601 tatgactgtc aggtccacat tctgtattcc ctcgaaaggc tggccacttc ctttccctgt
      661 aatcacgaac ttgtgcccgt ttacagcgcc ttccatacgc agctcgatct tcatttctga
      721 tgtaatcaca ctcaccatcc ggaattcttt gccaaaatga tgagacagca caataaccag
      781 cacgttgccc aggagctgta ggaaaaagaa gaaggcatga acatggttag cagaggctct
      841 agagccgccg gtcacacgcc agaagccgaa cccc
//
