LOCUS       sequence-424747-        6681 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses TALED_Left-mouse-ATP6-1397N in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6681)
  AUTHORS   Cho SI, Lim K, Hong S, Lee J, Kim A, Lim CJ, Ryou S, Lee JM, Mok YG,
            Chung E, Kim S, Han S, Cho SM, Kim J, Kim EK, Nam KH, Oh Y, Choi M, 
            An TH, Oh KJ, Lee S, Lee H, Kim JS
  TITLE     Engineering TALE-linked deaminases to facilitate precision adenine 
            base editing in mitochondrial DNA.
  JOURNAL   Cell. 2024 Jan 4;187(1):95-109.e26. doi: 10.1016/j.cell.2023.11.035.
  PUBMED    38181745
REFERENCE   2  (bases 1 to 6681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.11"; journalName: "Cell"; date: "2024-01-4- 4";
            volume: "187"; issue: "1"; pages: "95-109.e26"
COMMENT     Sequence Label: TALED_Left-mouse-ATP6-1397N.
FEATURES             Location/Qualifiers
     source          1..6681
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     149..168
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        243..347
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(348..416,417..1208)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(566..585)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1379..1967
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1868..1887
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2121..2138
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2255..2276
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2291..2296,2297..2314,2315..2321)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2329..2345
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2334..2356
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2353..2369
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2353..2369
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    2418..2539
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2429..2448)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2483..2502
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     polyA_signal    2770..2994
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2983..3000
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        3020..3038
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3020..3037
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(5829..5855)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(5859..5885)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(5889..5915)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(6011..6030)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6012..6030)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(6075..6278)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6104..6124)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6279..6658
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tcgcgaagca gcgcaaaacg cctaacccta agcagattct tcatgcaatt gtcggtcaag
       61 ccttgccttg ttgtagctta aattttgctc gcgcactact cagcgacctc caacacacaa
      121 gcagggagca gatactggct taactatgcg gcatcagagc agattgtact gagagtgcac
      181 cataggggat cgggagatct cccgatccgt cgacgtcagg tggcactttt cggggaaatg
      241 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      301 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      361 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      421 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      481 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      541 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      601 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      661 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      721 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
      781 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
      841 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
      901 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
      961 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1021 ctggctggtt tattgctgat aaatctggag ccggtgagcg tggatctcgc ggtatcattg
     1081 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1141 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1201 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1261 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1321 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1381 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1441 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1501 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     1561 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1621 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1681 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1741 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     1801 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     1861 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     1921 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     1981 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2041 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2101 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     2161 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     2221 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     2281 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     2341 aacaatttca cacaggaaac agctatgacc atgattacgc caagctctag ctagaggtcg
     2401 acggtataca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     2461 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     2521 aagctgcaat aaacaagttg gggtgggcga agaactccag catgagatcc ccgcgctgga
     2581 ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg
     2641 cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc
     2701 ccagagtccc gctcactgca gcgtggggat accccctaga gccccagctg gttctttccg
     2761 cctcagaagc catagagccc accgcatccc cagcatgcct gctattgtct tcccaatcct
     2821 cccccttgct gtcctgcccc accccacccc ccagaataga atgacaccta ctcagacaat
     2881 gcgatgcaat ttcctcattt tattaggaaa ggacagtggg agtggcacct tccagggtca
     2941 aggaaggcac gggggagggg caaacaacag atggctggca actagaaggc acagtcgagg
     3001 ctgatcagcg agctctagca tttaggtgac actatagaat agggccctga ctagccttca
     3061 ggtgggacga cagtcatctt ggcattttcg ggcagaaggg tttcagtcat attcacacag
     3121 aagccacaag taccctctgg gttgttatgg aagaccaacc cctcgcttat accattatcg
     3181 cgcataaaca gtgctgactg cccttctaca tgaccagcat tggcgtagtt ggggtagggt
     3241 gtagggcccc ctgaggagaa tactttggat tccagcccac cagcatcgtt tacgtagtaa
     3301 aaggtcccga cggtttgacc attataggcg gggagttggg gtgcgctaat ctggtaagga
     3361 ccaagtgcgt agctacctga tccagagccc cccaacccct ttttcacagc atccagggca
     3421 ggtcgtccgc caagacaagc aagagcaaca agatgatcat ttgtcaacgc ggctagcgcc
     3481 ggatcaggtc tggagagctg ggcaacaatg ctctctagag cctgtttgcc gccgccgttg
     3541 cttgcaatcg ccaccacctg ttccggagtc agaccgtgtg cttggcacaa cacgggaaga
     3601 agtctctgca cagtctccaa agcctgtttc ccaccgttgt tacttgcaat tgcgaccacc
     3661 tggtcgggag tcaatccatg ggcttgacaa agaactggca acaggcgctg gacagtttca
     3721 agggcttgct tccccccgat gttggaggca attgccacga cctgtgcagg ggtaagccca
     3781 tggtcctgac acaagacggg caacaaccgt tgcactgttt caagtgcctg tttgccaccc
     3841 ccattgctag cgatggcgac aacctgtgca ggtgtcaatc catgagcttg gcacaaaact
     3901 ggcagaagcc tttgcacagt ctccagggcc tgtttacccc cgtcgtgtga ggcgattgca
     3961 acaacttgtt cgggagtcaa tccgtgtgcc tggcagagaa cgggaagcaa ccgctggaca
     4021 gtttccaatg cttgcttccc cccatcatga ctggcgatag ccacgacttg tgcaggggtg
     4081 agcccatggt cttgacaaag gactggcaac aatctttgga ctgtttccag ggcctgctta
     4141 ccaccaccat tactggcaat ggcgacaact tgggctggag tcagcccatg gtcctgacac
     4201 aggactggca aaagtctctg aacggtttca agagcctgct tgcccccatc gtgtgaagca
     4261 atggccacca cctgatcagg agtgaggcca tgatcttggc acaagacggg aagcagcctt
     4321 tgcacagtct cgagggcttg cttgccgccg tcatgagagg cgattgcaac gacctggtcg
     4381 ggggtcaaac cgtgagcctg acaaagcacg gggagcagcc tctgcacagt ttcgagagcc
     4441 tgtttccccc catcatgact agcgatagcg acgacttgag ctggggtgag gccatgagct
     4501 tggcagagga caggcaaaag cctctggacg gtttccagtg cttgtttacc tcctatgttg
     4561 gatgcgatgg ccaccacctg tgctggagta agtccatgag cttggcaaag gacaggcaac
     4621 aatctttgaa ctgtttccaa agcctgtttg ccaccatcgt gtgaagcaat tgccacaact
     4681 tgctcgggtg tcaacccatg atcctgacaa agaacgggca gcaagcgttg gactgtctcc
     4741 aaggcctgct tccctcctcc attacttgca atagccacca cctgagctgg tgtaagacca
     4801 tgggcctggc aaaggacagg aagaagccgc tgaacggttt ccagggcctg tttgcccccg
     4861 ccatttgaag caattgcaac cacttggtct ggggtgagtc cgtgatcctg gcacaagact
     4921 ggcaacaatc tttggacagt ttccaaggcc tgcttgcctc cgatattgct tgcaatagca
     4981 acaacttgat cgggtgtgag cccatgtgct tggcagagga cgggaagcaa gcgctgcaca
     5041 gtctcaagag cttgcttccc tcctccattg ctggcaatgg ccacgacctg gtcaggtgtc
     5101 aacccatggt cctgacacaa aacagggagg agtctctgaa cagtttcaag agcttgtttc
     5161 cctccattat tgcttgcgat agcgacaacc tgggctggtg tcaacccgtg tgcctgacac
     5221 aagactggca agagccgttg cactgtctcg agggcttgct tacccccgtc gtgagaggca
     5281 attgccacca cctgatcagg agtgagtccg tgatcctggc aaagcacggg caacaaccgt
     5341 tgcacagttt cgagtgcctg tttcccgccg atgttggagg caatggcaac gacttggtct
     5401 ggggtcaagt tcaggggggc acccgtcagt gcattgcgcc atgcatgcac tgcctccact
     5461 gcggtcacgc cgccacgttt tgcaatcttg agaagttggc ctgtgtccaa ctgtaacggt
     5521 ggacctctca actctcccgc caccgtgagc aaggcctcca gagcgcgtgc gccggaccac
     5581 tgtttgccga cgccaacgat cgcttcgtgt gtcgcctctg gcaacgctgc gatcatgtcc
     5641 tgatacttga cagcgacggt ccctaacgct gccgggtgtt ggctgagcgc aacgatgtgc
     5701 gcgtgtgtaa acccatggcc gaccagtgcc tcgtggtgct gcgccactgt cgaacgaacc
     5761 ttcggtttga tcttctcctg ttgctgctgg ctgtagccga gcgtgcgtag atcttgaatt
     5821 cggatcccag cataatcagg gacatcgtat ggatatccag cgtaatctgg tacgtcgtaa
     5881 gggtagccgg cgtagtcagg tacatcgtaa gggtaatcag ggagagagtg cttttgtcgg
     5941 gagccaaggt accccaagac tggagcaagc tgtcgggaag taccacagac ggctcgacta
     6001 agagccatct ccctatagtg agtcgtatta atttcgataa gccagtaagc agtgggttct
     6061 ctagttagcc agagagctct gcttatatag acctcccacc gtacacgcct accgcccatt
     6121 tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa
     6181 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc
     6241 tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat
     6301 acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag
     6361 gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga
     6421 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
     6481 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
     6541 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg
     6601 ctatgaacta atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcaa
     6661 cgcgtatatc tggcccgtac a
//
