LOCUS       sequence-424749-        8185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  12xGCN4pep transgene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8185)
  TITLE     Cheeseman lab plasmids Nov 2023
REFERENCE   2  (bases 1 to 8185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGS402_myc-12xGCN4-T2A-BSD Lenti
FEATURES             Location/Qualifiers
     source          1..8185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    97..118
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(133..138,139..156,157..163)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    171..187
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     176..198
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     195..211
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     195..211
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     230..250
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        232..250
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        278..504
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             505..685
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    732..857
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1354..1587
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1772..1816
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     1994..2014
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    2215..2332
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2404..2615
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2644..2673
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2716..2736
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2749..2793
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             2830..2895
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             2911..2976
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             2992..3057
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3073..3138
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3154..3219
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3235..3300
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3316..3381
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3397..3462
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3478..3543
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3559..3624
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3640..3705
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3721..3786
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3811..3864
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3871..4269
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    4315..4903
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4368..4388)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4786..4797)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4975..5208
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5286..5407
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5323..5342)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5377..5396
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5447..5582
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5602..5621)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5603..5621)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5631..5648)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5631..5647)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5640..5662)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5789..6244
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5876..5895)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6086..6107
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6270..6374
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6375..6443,6444..7235)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6593..6612)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7406..7994
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7895..7914
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8148..8165
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
       61 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      121 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      181 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
      241 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
      301 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
      361 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
      421 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
      481 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
      541 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
      601 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
      661 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
      721 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
      781 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
      841 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
      901 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
      961 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1021 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1081 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1141 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1201 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     1261 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     1321 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     1381 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     1441 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     1501 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     1561 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     1621 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     1681 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     1741 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     1801 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     1861 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     1921 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     1981 gaggggaccc agagagggcc tatttcccat gattccttca tatttgcata tacgatacaa
     2041 ggctgttaga gagataatta gaattaattt gactgtaaac acaaagatat tagtacaaaa
     2101 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
     2161 aatggactat cataaattct agatcttgag acaaatggca gtattcatcc acaattttaa
     2221 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     2281 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     2341 ttacagggac agcagagatc cactttggcg ccggctcgtc gagtggctcc ggtgcccgtc
     2401 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
     2461 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     2521 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     2581 ttctttttcg caacgggttt gccgccagaa cacaggtgtc gtgacgcggg atccgatatc
     2641 atggagcaga agctgatcag cgaggaggac ctgggagggg atcggtggag ctccaccggt
     2701 ggcggccgct ctagagagaa tctttatttt cagggcgccg ccaaattcaa agaaaccgct
     2761 gctgctaaat tcgaacgcca gcacatggac agcggaggtg gaggtactag tctcgagggt
     2821 tctggaggat tgttgcccaa aaactaccac ttggagaacg aggtcgcaag gttgaaaaaa
     2881 ttggtcggtg agcgtggcgg ctcaggcggc cttcttccaa aaaattatca tcttgaaaat
     2941 gaagttgcta gacttaaaaa acttgttgga gaaagaggcg ggagcggcgg cctgctgccc
     3001 aagaactacc acctggagaa cgaggtcgcc cgcctgaaga agctggtcgg cgagcgcggt
     3061 ggttctggag gtttgctccc aaagaactac cacttggaaa acgaagtcgc aagattgaag
     3121 aagttggtcg gtgaaagagg aggaagcgga ggattattac ctaaaaatta tcatttggaa
     3181 aatgaagttg ctagattgaa gaagttggtt ggagagagag gggggtctgg aggactacta
     3241 ccgaaaaact accacctaga gaacgaggtg gcccgactaa aaaagctagt gggcgagcgc
     3301 ggcggcagtg gcggcctcct ccccaagaat taccacctcg aaaatgaagt agcaaggctc
     3361 aagaagctcg taggagaaag aggtggttcc gggggactcc ttccaaaaaa ctatcatctt
     3421 gaaaacgaag ttgcacgtct taaaaaactt gttggcgaac gtggcggctc tggcgggctg
     3481 ctgcccaaga attaccacct ggaaaatgaa gtggcccgcc tgaagaagct ggtgggcgaa
     3541 cgcggaggca gtggaggcct actaccgaag aactaccacc tagagaacga ggtggcacgc
     3601 ctaaagaagc tagtgggcga gcgcggcggc tcgggtggtt tattacctaa gaattatcat
     3661 ttagaaaatg aagtagctcg attaaagaag ttagtaggtg aacgtggtgg gtctggtggt
     3721 cttcttccga agaactacca ccttgagaac gaggtagctc gtcttaagaa acttgtaggt
     3781 gagcgtggtg gaagcggtgg tggggaattc gagggcagag gaagtcttct aacatgcggt
     3841 gacgtggagg agaatcccgg ccctatcgat atggccaagc ctttgtctca agaagaatcc
     3901 accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc
     3961 gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat
     4021 tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct
     4081 ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc
     4141 ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac
     4201 agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg
     4261 gagggctaag gtaccactag tcccgggatg catagcgcta cgcgttaagt cgacaatcaa
     4321 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     4381 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     4441 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     4501 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     4561 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     4621 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     4681 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     4741 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     4801 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     4861 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     4921 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     4981 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     5041 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     5101 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     5161 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta
     5221 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     5281 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     5341 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     5401 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     5461 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     5521 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     5581 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     5641 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     5701 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     5761 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     5821 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     5881 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     5941 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     6001 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     6061 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     6121 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     6181 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     6241 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     6301 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     6361 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     6421 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6481 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6541 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6601 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6661 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     6721 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     6781 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6841 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     6901 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     6961 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     7021 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     7081 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     7141 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     7201 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     7261 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     7321 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     7381 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7441 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7501 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     7561 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7621 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7681 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7741 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7801 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7861 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7921 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7981 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     8041 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     8101 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     8161 gcgaggaagc ggaagagcgc ccaat
//
