LOCUS       sequence-424754-        9269 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  8xGCN4pep transgene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9269)
  TITLE     Cheeseman lab plasmids Nov 2023
REFERENCE   2  (bases 1 to 9269)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGS378_tdTomato-8xGCN4-T2A-BSD Lenti
FEATURES             Location/Qualifiers
     source          1..9269
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     239..259
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        241..259
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        287..513
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             514..694
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    741..866
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1363..1596
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1781..1825
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     2003..2023
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    2224..2341
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2413..2624
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2651..2660
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2657..4084
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             4130..4150
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4163..4207
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             4244..4309
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4325..4390
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4406..4471
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4487..4552
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4568..4633
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4649..4714
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4730..4795
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4811..4876
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4904..4957
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4964..5362
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    5408..5996
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5461..5481)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5879..5890)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6068..6301
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6379..6500
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6416..6435)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6470..6489
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6540..6675
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6695..6714)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6696..6714)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6724..6741)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6724..6740)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6733..6755)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6882..7337
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6969..6988)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7179..7200
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7363..7467
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7468..7536,7537..8328)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7686..7705)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8499..9087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8988..9007
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9241..9258
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
      241 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
      301 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
      361 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
      421 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
      481 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
      541 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      601 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      661 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      721 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      781 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      841 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      901 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      961 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     1021 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1081 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1141 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1201 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1261 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1321 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1381 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1441 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1501 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1561 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1621 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1681 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1741 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1801 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1861 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1921 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1981 cccaaccccg aggggaccca gagagggcct atttcccatg attccttcat atttgcatat
     2041 acgatacaag gctgttagag agataattag aattaatttg actgtaaaca caaagatatt
     2101 agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt
     2161 atgttttaaa atggactatc ataaattcta gatcttgaga caaatggcag tattcatcca
     2221 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     2281 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     2341 tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgtcg agtggctccg
     2401 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
     2461 tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
     2521 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
     2581 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtgtcg tgacgcggga
     2641 tccgatatcc gccaccatgg tgagcaaggg cgaggaggtc atcaaagagt tcatgcgctt
     2701 caaggtgcgc atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga
     2761 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct
     2821 gcccttcgcc tgggacatcc tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa
     2881 gcaccccgcc gacatccccg attacaagaa gctgtccttc cccgagggct tcaagtggga
     2941 gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg acccaggact cctccctgca
     3001 ggacggcacg ctgatctaca aggtgaagat gcgcggcacc aacttccccc ccgacggccc
     3061 cgtaatgcag aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga
     3121 cggcgtgctg aagggcgaga tccaccaggc cctgaagctg aaggacggcg gccactacct
     3181 ggtggagttc aagaccatct acatggccaa gaagcccgtg caactgcccg gctactacta
     3241 cgtggacacc aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     3301 cgagcgctcc gagggccgcc accacctgtt cctggggcat ggcaccggca gcaccggcag
     3361 cggcagctcc ggcaccgcct cctccgagga caacaacatg gccgtcatca aagagttcat
     3421 gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga
     3481 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg
     3541 ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta
     3601 cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa
     3661 gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc
     3721 cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga
     3781 cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc
     3841 ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca
     3901 ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta
     3961 ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga
     4021 acagtacgag cgctccgagg gccgccacca cctgttcctg tacggcatgg acgagctgta
     4081 caagaccgga ggggatcggt ggagctccac cggtggcggc cgctctagag agaatcttta
     4141 ttttcagggc gccgccaaat tcaaagaaac cgctgctgct aaattcgaac gccagcacat
     4201 ggacagcgga ggtggaggta ctagtctcga gggttctgga ggattgttgc ccaaaaacta
     4261 ccacttggag aacgaggtcg caaggttgaa aaaattggtc ggtgagcgtg gcggctcagg
     4321 cggccttctt ccaaaaaatt atcatcttga aaatgaagtt gctagactta aaaaacttgt
     4381 tggagaaaga ggcgggagcg gcggcctgct gcccaagaac taccacctgg agaacgaggt
     4441 cgcccgcctg aagaagctgg tcggcgagcg cggtggttct ggaggtttgc tcccaaagaa
     4501 ctaccacttg gaaaacgaag tcgcaagatt gaagaagttg gtcggtgaaa gaggaggaag
     4561 cggaggatta ttacctaaaa attatcattt ggaaaatgaa gttgctagat tgaagaagtt
     4621 ggttggagag agaggggggt ctggaggact actaccgaaa aactaccacc tagagaacga
     4681 ggtggcccga ctaaaaaagc tagtgggcga gcgcggcggc agtggcggcc tcctccccaa
     4741 gaattaccac ctcgaaaatg aagtagcaag gctcaagaag ctcgtaggag aaagaggtgg
     4801 ttccggggga ctccttccaa aaaactatca tcttgaaaac gaagttgcac gtcttaaaaa
     4861 acttgttggc gaacgtggcg gctctggcgg gctgctggaa ttcgagggca gaggaagtct
     4921 tctaacatgc ggtgacgtgg aggagaatcc cggccctatc gatatggcca agcctttgtc
     4981 tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc
     5041 tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt
     5101 caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc
     5161 tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat
     5221 cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc
     5281 catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc
     5341 tggttatgtg tgggagggct aaggtaccac tagtcccggg atgcatagcg ctacgcgtta
     5401 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     5461 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     5521 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     5581 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     5641 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     5701 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     5761 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     5821 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     5881 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     5941 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg
     6001 actttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     6061 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     6121 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     6181 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     6241 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     6301 agtacgtata gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     6361 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     6421 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     6481 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     6541 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     6601 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     6661 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
     6721 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     6781 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     6841 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     6901 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     6961 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     7021 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     7081 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     7141 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     7201 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     7261 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     7321 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     7381 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     7441 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     7501 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     7561 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     7621 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     7681 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     7741 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     7801 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     7861 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     7921 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     7981 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     8041 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     8101 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     8161 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     8221 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     8281 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     8341 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     8401 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     8461 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     8521 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     8581 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     8641 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     8701 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     8761 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     8821 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     8881 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     8941 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     9001 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     9061 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     9121 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     9181 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     9241 agcgagtcag tgagcgagga agcggaaga
//
