LOCUS       sequence-424755-        8861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3xGCN4pep transgene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8861)
  TITLE     Cheeseman lab plasmids Nov 2023
REFERENCE   2  (bases 1 to 8861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGS375_tdTomato-3xGCN4-T2A-BSD Lenti
FEATURES             Location/Qualifiers
     source          1..8861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    91..112
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(127..132,133..150,151..157)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    165..181
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     170..192
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     189..205
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     189..205
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     224..244
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        226..244
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        272..498
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             499..679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    726..851
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1348..1581
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1766..1810
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     1988..2008
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    2209..2326
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2398..2609
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2636..2645
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2642..4069
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             4115..4135
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4148..4192
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             4229..4294
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4310..4375
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4391..4456
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4481..4534
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4541..4939
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    4985..5573
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5038..5058)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5456..5467)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5645..5878
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5956..6077
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5993..6012)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6047..6066
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6117..6252
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6272..6291)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6273..6291)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6301..6318)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6301..6317)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6310..6332)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6459..6914
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6546..6565)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6756..6777
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6940..7044
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7045..7113,7114..7905)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7263..7282)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8076..8664
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8565..8584
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8818..8835
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
       61 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
      121 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
      181 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
      241 aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct
      301 tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca
      361 tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt
      421 ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc
      481 ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
      541 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
      601 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
      661 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
      721 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
      781 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
      841 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
      901 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
      961 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     1021 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     1081 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     1141 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgct
     1201 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     1261 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     1321 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     1381 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     1441 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     1501 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     1561 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     1621 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     1681 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     1741 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     1801 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     1861 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     1921 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     1981 acccagagag ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt
     2041 tagagagata attagaatta atttgactgt aaacacaaag atattagtac aaaatacgtg
     2101 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga
     2161 ctatcataaa ttctagatct tgagacaaat ggcagtattc atccacaatt ttaaaagaaa
     2221 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2281 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     2341 ggacagcaga gatccacttt ggcgccggct cgtcgagtgg ctccggtgcc cgtcagtggg
     2401 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     2461 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2521 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2581 ttcgcaacgg gtttgccgcc agaacacagg tgtcgtgacg cgggatccga tatccgccac
     2641 catggtgagc aagggcgagg aggtcatcaa agagttcatg cgcttcaagg tgcgcatgga
     2701 gggctccatg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     2761 gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga
     2821 catcctgtcc ccccagttca tgtacggctc caaggcgtac gtgaagcacc ccgccgacat
     2881 ccccgattac aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     2941 cgaggacggc ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat
     3001 ctacaaggtg aagatgcgcg gcaccaactt cccccccgac ggccccgtaa tgcagaagaa
     3061 gaccatgggc tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg
     3121 cgagatccac caggccctga agctgaagga cggcggccac tacctggtgg agttcaagac
     3181 catctacatg gccaagaagc ccgtgcaact gcccggctac tactacgtgg acaccaagct
     3241 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc gctccgaggg
     3301 ccgccaccac ctgttcctgg ggcatggcac cggcagcacc ggcagcggca gctccggcac
     3361 cgcctcctcc gaggacaaca acatggccgt catcaaagag ttcatgcgct tcaaggtgcg
     3421 catggagggc tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     3481 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc
     3541 ctgggacatc ctgtcccccc agttcatgta cggctccaag gcgtacgtga agcaccccgc
     3601 cgacatcccc gattacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     3661 gaacttcgag gacggcggtc tggtgaccgt gacccaggac tcctccctgc aggacggcac
     3721 gctgatctac aaggtgaaga tgcgcggcac caacttcccc cccgacggcc ccgtaatgca
     3781 gaagaagacc atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct
     3841 gaagggcgag atccaccagg ccctgaagct gaaggacggc ggccactacc tggtggagtt
     3901 caagaccatc tacatggcca agaagcccgt gcaactgccc ggctactact acgtggacac
     3961 caagctggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgctc
     4021 cgagggccgc caccacctgt tcctgtacgg catggacgag ctgtacaaga ccggagggga
     4081 tcggtggagc tccaccggtg gcggccgctc tagagagaat ctttattttc agggcgccgc
     4141 caaattcaaa gaaaccgctg ctgctaaatt cgaacgccag cacatggaca gcggaggtgg
     4201 aggtactagt ctcgagggtt ctggaggatt gttgcccaaa aactaccact tggagaacga
     4261 ggtcgcaagg ttgaaaaaat tggtcggtga gcgtggcggc tcaggcggcc ttcttccaaa
     4321 aaattatcat cttgaaaatg aagttgctag acttaaaaaa cttgttggag aaagaggcgg
     4381 gagcggcggc ctgctgccca agaactacca cctggagaac gaggtcgccc gcctgaagaa
     4441 gctggtcggc gagcgcggtg gttctggagg tttggaattc gagggcagag gaagtcttct
     4501 aacatgcggt gacgtggagg agaatcccgg ccctatcgat atggccaagc ctttgtctca
     4561 agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga
     4621 agactacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa
     4681 tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc
     4741 tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt
     4801 gagcccctgc ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat
     4861 agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg
     4921 ttatgtgtgg gagggctaag gtaccactag tcccgggatg catagcgcta cgcgttaagt
     4981 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     5041 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     5101 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     5161 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     5221 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     5281 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     5341 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     5401 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     5461 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     5521 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact
     5581 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     5641 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     5701 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     5761 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     5821 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     5881 acgtatagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     5941 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     6001 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     6061 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc
     6121 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     6181 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     6241 tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc
     6301 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     6361 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     6421 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
     6481 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     6541 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     6601 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     6661 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     6721 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     6781 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     6841 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     6901 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     6961 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     7021 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     7081 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     7141 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     7201 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     7261 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     7321 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     7381 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     7441 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     7501 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     7561 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     7621 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     7681 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     7741 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     7801 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     7861 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     7921 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     7981 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     8041 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     8101 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     8161 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     8221 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     8281 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     8341 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     8401 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     8461 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     8521 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     8581 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     8641 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     8701 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     8761 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     8821 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca a
//
