LOCUS       sequence-424759-        3933 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-PRKAR1A
FEATURES             Location/Qualifiers
     source          1..3933
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             144..935
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1028..1616
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1517..1536
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1770..1787
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1946..1973)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2065..2151)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2200..2222
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2214..2231
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2215..2231
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2247..2346
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3490..3589)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3606..3625)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3607..3625)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3630..3646)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3630..3646)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
       61 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
      121 gccctaaaac aaagttaaac attatgaggg aagcggtgat cgccgaagta tcgactcaac
      181 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
      241 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
      301 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
      361 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
      421 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
      481 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
      541 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
      601 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
      661 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
      721 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
      781 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
      841 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg
      901 tgaaaggcga gatcaccaag gtagtcggca aataaccctc gagccaccca tgaccaaaat
      961 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1021 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1081 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1141 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     1201 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1261 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1321 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1381 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1441 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1501 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1561 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     1621 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     1681 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     1741 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     1801 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     1861 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta
     1921 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
     1981 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac
     2041 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     2101 acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc
     2161 agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga cgttgtaaaa
     2221 cgacggccag tcttaagctc gggccccaaa taatgatttt attttgactg atagtgacct
     2281 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
     2341 ttggcatgga gtctggcagt accgccgcca gtgaggaggc acgcagcctt cgagaatgtg
     2401 agctctacgt ccagaagcat aacattcaag cgctgctcaa agattctatt gtgcagttgt
     2461 gcactgctcg acctgagaga cccatggcat tcctcaggga atactttgag aggttggaga
     2521 aggaggaggc aaaacagatt cagaatctgc agaaagcagg cactcgtaca gactcaaggg
     2581 aggatgagat ttctcctcct ccacccaacc cagtggttaa aggtaggagg cgacgaggtg
     2641 ctatcagcgc tgaggtctac acggaggaag atgcggcatc ctatgttaga aaggttatac
     2701 caaaagatta caagacaatg gccgctttag ccaaagccat tgaaaagaat gtgctgtttt
     2761 cacatcttga tgataatgag agaagtgata tttttgatgc catgttttcg gtctccttta
     2821 tcgcaggaga gactgtgatt cagcaaggtg atgaagggga taacttctat gtgattgatc
     2881 aaggagagac ggatgtctat gttaacaatg aatgggcaac cagtgttggg gaaggaggga
     2941 gctttggaga acttgctttg atttatggaa caccgagagc agccactgtc aaagcaaaga
     3001 caaatgtgaa attgtggggc atcgaccgag acagctatag aagaatcctc atgggaagca
     3061 cactgagaaa gcggaagatg tatgaggaat tccttagtaa agtctctatt ttagagtctc
     3121 tggacaagtg ggaacgtctt acggtagctg atgcattgga accagtgcag tttgaagatg
     3181 ggcagaagat tgtggtgcag ggagaaccag gggatgagtt cttcattatt ttagaggggt
     3241 cagctgctgt gctacaacgt cggtcagaaa atgaagagtt tgttgaagtg ggaagattgg
     3301 ggccttctga ttattttggt gaaattgcac tactgatgaa tcgtcctcgt gctgccacag
     3361 ttgttgctcg tggccccttg aagtgcgtta agctggaccg acctagattt gaacgtgttc
     3421 ttggcccatg ctcagacatc ctcaaacgaa acatccagca gtacaacagt tttgtgtcac
     3481 tgtctgtctg cccaactttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa
     3541 tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg
     3601 atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg
     3661 tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca tgcctcctct
     3721 agaccagcca ggacagaaat gcctcgactt cgctgctacc caaggttgcc gggtgacgca
     3781 caccgtggaa acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc
     3841 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
     3901 gtggtaacgg cgcagtggcg gttttcatgg ctt
//
