LOCUS       sequence-42476-e         518 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 518)
  AUTHORS   He A, Kong SW, Ma Q, Pu WT
  TITLE     Co-occupancy by multiple cardiac transcription factors identifies 
            transcriptional enhancers active in heart.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Apr 5. 108(14):5632-7.
  PUBMED    21415370
REFERENCE   2  (bases 1 to 518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2011 Apr 5. 108(14):5632-7."
COMMENT     Sequence Label: pEntr-Nkx2-5flbio
FEATURES             Location/Qualifiers
     source          1..518
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    89..188
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(233..277)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             complement(290..313)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agttttattg ttcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga
       61 gacacgggcc agagctgcag ctggatggca aataatgatt ttattttgac tgatagtgac
      121 ctgttcgttg caacaaattg ataagcaatg ctttcttata atgccaactt tgtacaagaa
      181 agctgggtct agatatctcg agtgcggccg cctacctcga gctcggcgcg ccttcgtgcc
      241 attcgatttt ctgagcctcg aagatgtcgt tcaggccgga cccatcacct ttatcgtcat
      301 cgtctttgta gtcacgcgtc ggtccgctcc aggctcggat gccgtgcagc gtggagacgc
      361 ccgaattgcc ctgcggcatc ccgggactct gcacggtgtt caagtccccg acgccaaagt
      421 tcacgaagtt gctgttggcg gaggcggcgg ggggctgcgc ggcggggggc gcagcggggt
      481 aggcggcgca gctgtagccg ggactacagg ccgcgccg
//
