LOCUS       sequence-424768-        1151 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of CREB1 domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Martinez-Yamout MA, Nasir I, Shnitkind S, Ellis JP, Berlow RB, Kroon
            G, Deniz AA, Dyson HJ, Wright PE
  TITLE     Glutamine-rich regions of the disordered CREB transactivation domain
            mediate dynamic intra- and intermolecular interactions.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Nov 21;120(47):e2313835120. doi: 
            10.1073/pnas.2313835120. Epub 2023 Nov 14.
  PUBMED    37971402
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-11-21"; volume: "120(47):e2313835120. 
            doi"; pages: " 10.1073/pnas.2313835120. Epub 2023 Nov 14"
COMMENT     Sequence Label: H6Gb1-CREB(1-280)
FEATURES             Location/Qualifiers
     source          1..1151
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             10..32
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..239
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             264..284
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ctagaatatt ttgtttaact ttaagaagga gatataccat gggccatcat catcatcatc
       61 acagcagcgg catgacttac aaactgatcc ttaacggtaa aactttgaaa ggcgaaacca
      121 ctaccgaagc tgttgatgct gcgactgcag aaaaagtttt caaacagtac gctaacgaca
      181 acggtgttga cggtgaatgg acttacgacg atgcgactaa gaccttcacc gttactgaag
      241 aattcagcag cggcagcagc ggcgaaaacc tgtattttca gggctcccat atgaccatgg
      301 actctggagc agacaaccag cagagtggag atgctgctgt aacagaagct gaaagtcaac
      361 aaatgacagt tcaagcccag ccacagattg ccacattagc ccaggtatcc atgccagcag
      421 ctcatgcgac gtcatctgct cccactgtaa ccttagtgca gctgcccaat gggcagacag
      481 tccaggtcca tggggtcatc caggcggccc agccatcagt tattcagtct ccacaagtcc
      541 aaacagttca gtcttcctgt aaggacttaa aaagactttt ctccggaact cagatttcaa
      601 ctattgcaga aagtgaagat tcacaggagt ctgtggatag tgtaactgat tcccaaaaac
      661 gaagggaaat cctttcaagg aggccttcct acaggaaaat tttgaatgac ttatcttctg
      721 atgcaccagg ggtgccaagg attgaagaag aaaaatcaga agaagagact tcagcccctg
      781 ccatcaccac tgtaacagtg ccaaccccga tttaccaaac tagcagtggg cagtatattg
      841 ccattaccca gggaggagca atacagctgg ctaacaatgg taccgatggg gtacagggcc
      901 tgcagacatt aaccatgacc aatgcagctg ccactcagcc gggtactacc attctacaat
      961 atgcacagac cactgatgga cagcagattc tagtgcccag caaccaagtt gttgttcaag
     1021 ctgcctctgg tgatgtacaa acataccaga ttcgcacagc acccactagc accattgccc
     1081 ctggagttgt tatggcgtcc tccccagcac ttcctacaca gcctgctgaa taatagaagc
     1141 ttgcgccgca c
//
