LOCUS       sequence-424770-        1114 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of CREB1 domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Martinez-Yamout MA, Nasir I, Shnitkind S, Ellis JP, Berlow RB, Kroon
            G, Deniz AA, Dyson HJ, Wright PE
  TITLE     Glutamine-rich regions of the disordered CREB transactivation domain
            mediate dynamic intra- and intermolecular interactions.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Nov 21;120(47):e2313835120. doi: 
            10.1073/pnas.2313835120. Epub 2023 Nov 14.
  PUBMED    37971402
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-11-21"; volume: "120(47):e2313835120. 
            doi"; pages: " 10.1073/pnas.2313835120. Epub 2023 Nov 14"
COMMENT     Sequence Label: H6Gb1-CREB(1-280) C90A, T172C
FEATURES             Location/Qualifiers
     source          1..1114
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             8..25
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             41..202
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             227..247
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 catgggccat catcatcatc atcacagcag cggcatgact tacaaactga tccttaacgg
       61 taaaactttg aaaggcgaaa ccactaccga agctgttgat gctgcgactg cagaaaaagt
      121 tttcaaacag tacgctaacg acaacggtgt tgacggtgaa tggacttacg acgatgcgac
      181 taagaccttc accgttactg aagaattcag cagcggcagc agcggcgaaa acctgtattt
      241 tcagggctcc catatgacca tggactctgg agcagacaac cagcagagtg gagatgctgc
      301 tgtaacagaa gctgaaagtc aacaaatgac agttcaagcc cagccacaga ttgccacatt
      361 agcccaggta tccatgccag cagctcatgc gacgtcatct gctcccactg taaccttagt
      421 gcagctgccc aatgggcaga cagtccaggt ccatggggtc atccaggcgg cccagccatc
      481 agttattcag tctccacaag tccaaacagt tcagtcttcc gcgaaggact taaaaagact
      541 tttctccgga actcagattt caactattgc agaaagtgaa gattcacagg agtctgtgga
      601 tagtgtaact gattcccaaa aacgaaggga aatcctttca aggaggcctt cctacaggaa
      661 aattttgaat gacttatctt ctgatgcacc aggggtgcca aggattgaag aagaaaaatc
      721 agaagaagag acttcagccc ctgccatcac cactgtaaca gtgccatgcc cgatttacca
      781 aactagcagt gggcagtata ttgccattac ccagggagga gcaatacagc tggctaacaa
      841 tggtaccgat ggggtacagg gcctgcagac attaaccatg accaatgcag ctgccactca
      901 gccgggtact accattctac aatatgcaca gaccactgat ggacagcaga ttctagtgcc
      961 cagcaaccaa gttgttgttc aagctgcctc tggtgatgta caaacatacc agattcgcac
     1021 agcacccact agcaccattg cccctggagt tgttatggcg tcctccccag cacttcctac
     1081 acagcctgct gaataataga agcttgcgcc gcac
//
