LOCUS       sequence-424772-        7363 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV packaging vector containing AAV2-rep gene and CapDJR modified 
            AAV-DJ8 capsid coding sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7363)
  AUTHORS   Chen Y, Wang J, Liu J, Lin J, Lin Y, Nie J, Yue Q, Deng C, Qi X, Li 
            Y, Dai J, Lu Z
  TITLE     A Novel Retrograde AAV Variant for Functional Manipulation of 
            Cortical Projection Neurons in Mice and Monkeys.
  JOURNAL   Neurosci Bull. 2023 Jul 11. doi: 10.1007/s12264-023-01091-0.
  PUBMED    37432585
REFERENCE   2  (bases 1 to 7363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neurosci 
            Bull. 2023 Jul 11. doi: 10.1007/s12264-023-01091-0."
COMMENT     Sequence Label: AAV-DJ8R
FEATURES             Location/Qualifiers
     source          1..7363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3973..3984
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(4436..4456)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4436..4454)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4475..4491)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4475..4491)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4488..4510)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4499..4515
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4523..4529,4530..4547,4548..4553))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4568..4589
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4706..4723)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4877..5465)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4957..4976)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5636..6427,6428..6496))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6259..6278
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6497..6601)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6627..7082)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6764..6785)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6976..6995
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7209..7231
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7223..7240
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7224..7240
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7250..7269
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7250..7268
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     7297..7313
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     7298..7314
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ccgccatgcc ggggttttac gagattgtga ttaaggtccc cagcgacctt gacgagcatc
       61 tgcccggcat ttctgacagc tttgtgaact gggtggccga gaaggaatgg gagttgccgc
      121 cagattctga catggatctg aatctgattg agcaggcacc cctgaccgtg gccgagaagc
      181 tgcagcgcga ctttctgacg gaatggcgcc gtgtgagtaa ggccccggag gctcttttct
      241 ttgtgcaatt tgagaaggga gagagctact tccacatgca cgtgctcgtg gaaaccaccg
      301 gggtgaaatc catggttttg ggacgtttcc tgagtcagat tcgcgaaaaa ctgattcaga
      361 gaatttaccg cgggatcgag ccgactttgc caaactggtt cgcggtcaca aagaccagaa
      421 atggcgccgg aggcgggaac aaggtggtgg atgagtgcta catccccaat tacttgctcc
      481 ccaaaaccca gcctgagctc cagtgggcgt ggactaatat ggaacagtat ttaagcgcct
      541 gtttgaatct cacggagcgt aaacggttgg tggcgcagca tctgacgcac gtgtcgcaga
      601 cgcaggagca gaacaaagag aatcagaatc ccaattctga tgcgccggtg atcagatcaa
      661 aaacttcagc caggtacatg gagctggtcg ggtggctcgt ggacaagggg attacctcgg
      721 agaagcagtg gatccaggag gaccaggcct catacatctc cttcaatgcg gcctccaact
      781 cgcggtccca aatcaaggct gccttggaca atgcgggaaa gattatgagc ctgactaaaa
      841 ccgcccccga ctacctggtg ggccagcagc ccgtggagga catttccagc aatcggattt
      901 ataaaatttt ggaactaaac gggtacgatc cccaatatgc ggcttccgtc tttctgggat
      961 gggccacgaa aaagttcggc aagaggaaca ccatctggct gtttgggcct gcaactaccg
     1021 ggaagaccaa catcgcggag gccatagccc acactgtgcc cttctacggg tgcgtaaact
     1081 ggaccaatga gaactttccc ttcaacgact gtgtcgacaa gatggtgatc tggtgggagg
     1141 aggggaagat gaccgccaag gtcgtggagt cggccaaagc cattctcgga ggaagcaagg
     1201 tgcgcgtgga ccagaaatgc aagtcctcgg cccagataga cccgactccc gtgatcgtca
     1261 cctccaacac caacatgtgc gccgtgattg acgggaactc aacgaccttc gaacaccagc
     1321 agccgttgca agaccggatg ttcaaatttg aactcacccg ccgtctggat catgactttg
     1381 ggaaggtcac caagcaggaa gtcaaagact ttttccggtg ggcaaaggat cacgtggttg
     1441 aggtggagca tgaattctac gtcaaaaagg gtggagccaa gaaaagaccc gcccccagtg
     1501 acgcagatat aagtgagccc aaacgggtgc gcgagtcagt tgcgcagcca tcgacgtcag
     1561 acgcggaagc ttcgatcaac tacgcagaca ggtaccaaaa caaatgttct cgtcacgtgg
     1621 gcatgaatct gatgctgttt ccctgcagac aatgcgagag aatgaatcag aattcaaata
     1681 tctgcttcac tcacggacag aaagactgtt tagagtgctt tcccgtgtca gaatctcaac
     1741 ccgtttctgt cgtcaaaaag gcgtatcaga aactgtgcta cattcatcat atcatgggaa
     1801 aggtgccaga cgcttgcact gcctgcgatc tggtcaatgt ggatttggat gactgcatct
     1861 ttgaacaata aatgatttaa atcaggtatg gctgccgatg gttatcttcc agattggctc
     1921 gaggacactc tctctgaagg aataagacag tggtggaagc tcaaacctgg cccaccacca
     1981 ccaaagcccg cagagcggca taaggacgac agcaggggtc ttgtgcttcc tgggtacaag
     2041 tacctcggac ccttcaacgg actcgacaag ggagagccgg tcaacgaggc agacgccgcg
     2101 gccctcgagc acgacaaagc ctacgaccgg cagctcgaca gcggagacaa cccgtacctc
     2161 aagtacaacc acgccgacgc cgagttccag gagcggctca aagaagatac gtcttttggg
     2221 ggcaacctcg ggcgagcagt cttccaggcc aaaaagaggc ttcttgaacc tcttggtctg
     2281 gttgaggaag cggctaagac ggctcctgga aagaagaggc ctgtagagca ctctcctgtg
     2341 gagccagact cctcctcggg aaccggaaag gcgggccagc agcctgcaag aaaaagattg
     2401 aattttggtc agactggaga cgcagactca gtcccagacc ctcaaccaat cggagaacct
     2461 cccgcagccc cctcaggtgt gggatctctt acaatggctg caggcggtgg cgcaccaatg
     2521 gcagacaata acgagggcgc cgacggagtg ggtaattcct cgggaaattg gcattgcgat
     2581 tccacatgga tgggcgacag agtcatcacc accagcaccc gaacctgggc cctgcccacc
     2641 tacaacaacc acctctacaa gcaaatctcc aacagcacat ctggaggatc ttcaaatgac
     2701 aacgcctact tcggctacag caccccctgg gggtattttg actttaacag attccactgc
     2761 cacttttcac cacgtgactg gcagcgactc atcaacaaca actggggatt ccggcccaag
     2821 agactcagct tcaagctctt caacatccag gtcaaggagg tcacgcagaa tgaaggcacc
     2881 aagaccatcg ccaataacct caccagcacc atccaggtgt ttacggactc ggagtaccag
     2941 ctgccgtacg ttctcggctc tgcccaccag ggctgcctgc ctccgttccc ggcggacgtg
     3001 ttcatgattc cccagtacgg ctacctaaca ctcaacgacg gtagtcaggc cgtgggacgc
     3061 tcctccttct actgcctgga atactttcct tcgcagatgc tgagaaccgg caacaacttc
     3121 cagtttactt acaccttcga ggacgtgcct ttccacagca gctacgccca cagccagagc
     3181 ttggaccggc tgatgaatcc tctgattgac cagtacctgt actacttgtc tcggactcaa
     3241 acaacaggag gcacgacaaa tacgcagact ctgggcttca gccaaggtgg gcctaataca
     3301 atggccaatc aggcaaagaa ctggctgcca ggaccctgtt accgccagca gcgagtatca
     3361 aagacatctg cggataacaa caacagtgaa tactcgtgga ctggagctac caagtaccac
     3421 ctcaatggca gagactctct ggtgaatccg ggcccggcca tggcaagcca caaggacgat
     3481 gaagaaaagt tttttcctca gagcggggtt ctcatctttg ggaagcaagg ctcagagaaa
     3541 acaaatgtgg acattgaaaa ggtcatgatt acagacgaag aggaaatcag gacaaccaat
     3601 cccgtggcta cggagcagta tggttctgta tctaccaacc tccagcaagg caacctagca
     3661 gaccaagact acacaaaaac tgctacacaa gcagctaccg cagatgtcaa cacacaaggc
     3721 gttcttccag gcatggtctg gcaggacaga gatgtgtacc ttcaggggcc catctgggca
     3781 aagattccac acacggacgg acattttcac ccctctcccc tcatgggtgg attcggactt
     3841 aaacaccctc cgcctcagat cctgatcaag aacacgcctg tacctgcgga tcctccgacc
     3901 accttcaacc agtcaaagct gaactctttc atcacccagt attctactgg ccaagtcagc
     3961 gtggagatcg agtgggagct gcagaaggaa aacagcaagc gctggaaccc cgagatccag
     4021 tacacctcca actactacaa atctatcaac gtggactttg ctgttaatac agaaggcgtg
     4081 tactctgaac cccgccccat tggcacccgt tacctcaccc gtaatctgta attgcctgtt
     4141 aatcaataaa ccggttgatt cgtttcagtt gaactttggt ctctgcgaag ggcgaattcg
     4201 tttaaacctg caggactaga ggtcctgtat tagaggtcac gtgagtgttt tgcgacattt
     4261 tgcgacacca tgtggtcacg ctgggtattt aagcccgagt gagcacgcag ggtctccatt
     4321 ttgaagcggg aggtttgaac gcgcagccgc caagccgaat tctgcagata tccatcacac
     4381 tggcggccgc tcgactagag cggccgccac cgcggtggag ctccagcttt tgttcccttt
     4441 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     4501 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     4561 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     4621 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     4681 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     4741 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     4801 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     4861 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     4921 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     4981 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     5041 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     5101 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     5161 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     5221 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     5281 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     5341 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     5401 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     5461 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     5521 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     5581 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     5641 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     5701 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     5761 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     5821 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     5881 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     5941 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     6001 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     6061 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     6121 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     6181 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     6241 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     6301 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     6361 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     6421 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     6481 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     6541 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     6601 gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc
     6661 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     6721 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     6781 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     6841 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     6901 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     6961 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     7021 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     7081 gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     7141 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     7201 agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc
     7261 actatagggc gaattgggta ccgggccccc cctcgatcga ggtcgacggt atcgggggag
     7321 ctcgcagggt ctccattttg aagcgggagg tttgaacgcg cag
//
