LOCUS       sequence-424773-        7366 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV packaging vector carrying AAV2 rep gene and Cap8R modified AAV8 
            capsid coding sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7366)
  AUTHORS   Chen Y, Hong Z, Wang J, Liu K, Liu J, Lin J, Feng S, Zhang T, Shan 
            L, Liu T, Guo P, Lin Y, Li T, Chen Q, Jiang X, Li A, Li X, Li Y, 
            Wilde JJ, Bao J, Dai J, Lu Z
  TITLE     Circuit-specific gene therapy reverses core symptoms in a primate 
            Parkinson's disease model.
  JOURNAL   Cell. 2023 Nov 22;186(24):5394-5410.e18. doi: 
            10.1016/j.cell.2023.10.004. Epub 2023 Nov 2.
  PUBMED    37922901
REFERENCE   2  (bases 1 to 7366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.10.004"; journalName: "Cell"; date: 
            "2023-11-22- 22"; volume: "186"; issue: "24"; pages: "5394-5410.e18"
COMMENT     Sequence Label: AAV8R-12 Capsid
FEATURES             Location/Qualifiers
     source          1..7366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3971..3982
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(4434..4454)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4434..4452)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4473..4489)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4473..4489)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4486..4508)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4497..4513
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4521..4527,4528..4545,4546..4551))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4566..4587
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4704..4721)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4875..5463)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4955..4974)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5634..6425,6426..6494))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6257..6276
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6495..6599)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6625..7080)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6762..6783)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6974..6993
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7207..7229
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7221..7238
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7222..7238
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7248..7267
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7248..7266
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     7295..7311
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     7296..7312
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atgccggggt tttacgagat tgtgattaag gtccccagcg accttgacga gcatctgccc
       61 ggcatttctg acagctttgt gaactgggtg gccgagaagg aatgggagtt gccgccagat
      121 tctgacatgg atctgaatct gattgagcag gcacccctga ccgtggccga gaagctgcag
      181 cgcgactttc tgacggaatg gcgccgtgtg agtaaggccc cggaggctct tttctttgtg
      241 caatttgaga agggagagag ctacttccac atgcacgtgc tcgtggaaac caccggggtg
      301 aaatccatgg ttttgggacg tttcctgagt cagattcgcg aaaaactgat tcagagaatt
      361 taccgcggga tcgagccgac tttgccaaac tggttcgcgg tcacaaagac cagaaatggc
      421 gccggaggcg ggaacaaggt ggtggatgag tgctacatcc ccaattactt gctccccaaa
      481 acccagcctg agctccagtg ggcgtggact aatatggaac agtatttaag cgcctgtttg
      541 aatctcacgg agcgtaaacg gttggtggcg cagcatctga cgcacgtgtc gcagacgcag
      601 gagcagaaca aagagaatca gaatcccaat tctgatgcgc cggtgatcag atcaaaaact
      661 tcagccaggt acatggagct ggtcgggtgg ctcgtggaca aggggattac ctcggagaag
      721 cagtggatcc aggaggacca ggcctcatac atctccttca atgcggcctc caactcgcgg
      781 tcccaaatca aggctgcctt ggacaatgcg ggaaagatta tgagcctgac taaaaccgcc
      841 cccgactacc tggtgggcca gcagcccgtg gaggacattt ccagcaatcg gatttataaa
      901 attttggaac taaacgggta cgatccccaa tatgcggctt ccgtctttct gggatgggcc
      961 acgaaaaagt tcggcaagag gaacaccatc tggctgtttg ggcctgcaac taccgggaag
     1021 accaacatcg cggaggccat agcccacact gtgcccttct acgggtgcgt aaactggacc
     1081 aatgagaact ttcccttcaa cgactgtgtc gacaagatgg tgatctggtg ggaggagggg
     1141 aagatgaccg ccaaggtcgt ggagtcggcc aaagccattc tcggaggaag caaggtgcgc
     1201 gtggaccaga aatgcaagtc ctcggcccag atagacccga ctcccgtgat cgtcacctcc
     1261 aacaccaaca tgtgcgccgt gattgacggg aactcaacga ccttcgaaca ccagcagccg
     1321 ttgcaagacc ggatgttcaa atttgaactc acccgccgtc tggatcatga ctttgggaag
     1381 gtcaccaagc aggaagtcaa agactttttc cggtgggcaa aggatcacgt ggttgaggtg
     1441 gagcatgaat tctacgtcaa aaagggtgga gccaagaaaa gacccgcccc cagtgacgca
     1501 gatataagtg agcccaaacg ggtgcgcgag tcagttgcgc agccatcgac gtcagacgcg
     1561 gaagcttcga tcaactacgc agacaggtac caaaacaaat gttctcgtca cgtgggcatg
     1621 aatctgatgc tgtttccctg cagacaatgc gagagaatga atcagaattc aaatatctgc
     1681 ttcactcacg gacagaaaga ctgtttagag tgctttcccg tgtcagaatc tcaacccgtt
     1741 tctgtcgtca aaaaggcgta tcagaaactg tgctacattc atcatatcat gggaaaggtg
     1801 ccagacgctt gcactgcctg cgatctggtc aatgtggatt tggatgactg catctttgaa
     1861 caataaatga tttaaatcag gtatggctgc cgatggttat cttccagatt ggctcgagga
     1921 caacctctct gagggcattc gcgagtggtg ggcgctgaaa cctggagccc cgaagcccaa
     1981 agccaaccag caaaagcagg acgacggccg gggtctggtg cttcctggct acaagtacct
     2041 cggacccttc aacggactcg acaaggggga gcccgtcaac gcggcggacg cagcggccct
     2101 cgagcacgac aaggcctacg accagcagct gcaggcgggt gacaatccgt acctgcggta
     2161 taaccacgcc gacgccgagt ttcaggagcg tctgcaagaa gatacgtctt ttgggggcaa
     2221 cctcgggcga gcagtcttcc aggccaagaa gcgggttctc gaacctctcg gtctggttga
     2281 ggaaggcgct aagacggctc ctggaaagaa gagaccggta gagccatcac cccagcgttc
     2341 tccagactcc tctacgggca tcggcaagaa aggccaacag cccgccagaa aaagactcaa
     2401 ttttggtcag actggcgact cagagtcaga gccagaccct caacctctcg gagaacctcc
     2461 agcagcgccc tctggtgtgg gacctaatac aatggctgca ggcggtggcg caccaatggc
     2521 agacaataac gaaggcgccg acggagtggg tagttcctcg ggaaattggc attgcgattc
     2581 cacatggctg ggcgacagag tcatcaccac cagcacccga acctgggccc tgcccaccta
     2641 caacaaccac ctctacaagc aaatctccaa cgggacatcg ggaggagcca ccaacgacaa
     2701 cacctacttc ggctacagca ccccctgggg gtattttgac tttaacagat tccactgcca
     2761 cttttcacca cgtgactggc agcgactcat caacaacaac tggggattcc ggcccaagag
     2821 actcagcttc aagctcttca acatccaggt caaggaggtc acgcagaatg aaggcaccaa
     2881 gaccatcgcc aataacctca ccagcaccat ccaggtgttt acggactcgg agtaccagct
     2941 gccgtacgtt ctcggctctg cccaccaggg ctgcctgcct ccgttcccgg cggacgtgtt
     3001 catgattccc cagtacggct acctaacact caacgacggt agtcaggccg tgggacgctc
     3061 ctccttctac tgcctggaat actttccttc gcagatgctg agaaccggca acagcttcca
     3121 gtttacttac accttcgagg acgtgccttt ccacagcagc tacgcccaca gccagagctt
     3181 ggaccggctg atgaatcctc tgattgacca gtacctgtac tacttgtctc ggactcaaac
     3241 aacaggaggc acggcaaata cgcagactct gggcttcagc caaggtgggc ctaatacaat
     3301 ggccaatcag gcaaagaact ggctgccagg accctgttac cgccaacaac gcgtctcaac
     3361 gacaaccggg caaaacaaca atagcaactt tgcctggact gctgggacca aataccatct
     3421 gaatggaaga aattcattgg ctaatcctgg catcgctatg gcaacacaca aagacgacga
     3481 ggagcgtttt tttcccagta acgggatcct gatttttggc aaacaaaatg ctgccagaga
     3541 caatgcggat tacagcgatg tcatgctcac cagcgaggaa gaaatcaaaa ccactaaccc
     3601 tgtggctaca gaggaatacg gtatcgtggc agataacttg cagagaggca acctagcaga
     3661 ccaagactac acaaaaactg ctaggcaagc agctaccgca gatgtcaaca gccagggggc
     3721 cttacccggt atggtctggc agaaccggga cgtgtacctg cagggtccca tctgggccaa
     3781 gattcctcac acggacggca acttccaccc gtctccgctg atgggcggct ttggcctgaa
     3841 acatcctccg cctcagatcc tgatcaagaa cacgcctgta cctgcggatc ctccgaccac
     3901 cttcaaccag tcaaagctga actctttcat cacgcaatac agcaccggac aggtcagcgt
     3961 ggaaattgaa tgggagctgc agaaggaaaa cagcaagcgc tggaaccccg agatccagta
     4021 cacctccaac tactacaaat ctattaatgt ggactttgct gttaatacag aaggcgtgta
     4081 ctctgaaccc cgccccattg gcacccgtta cctcacccgt aatctgtaat tgcctgttaa
     4141 tcaataaacc ggttgattcg tttcagttga actttggtct ctgcgaaggg cgaattcgtt
     4201 taaacctgca ggactagagg tcctgtatta gaggtcacgt gagtgttttg cgacattttg
     4261 cgacaccatg tggtcacgct gggtatttaa gcccgagtga gcacgcaggg tctccatttt
     4321 gaagcgggag gtttgaacgc gcagccgcca agccgaattc tgcagatatc catcacactg
     4381 gcggccgctc gactagagcg gccgccaccg cggtggagct ccagcttttg ttccctttag
     4441 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     4501 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     4561 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     4621 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     4681 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     4741 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     4801 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     4861 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     4921 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     4981 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     5041 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     5101 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     5161 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     5221 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     5281 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     5341 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     5401 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     5461 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     5521 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     5581 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     5641 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     5701 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     5761 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     5821 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     5881 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     5941 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     6001 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     6061 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     6121 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     6181 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     6241 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     6301 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     6361 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     6421 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     6481 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     6541 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     6601 acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt
     6661 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     6721 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     6781 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     6841 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     6901 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     6961 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     7021 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     7081 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     7141 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     7201 ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac
     7261 tatagggcga attgggtacc gggccccccc tcgatcgagg tcgacggtat cgggggagct
     7321 cgcagggtct ccattttgaa gcgggaggtt tgaacgcgca gccgcc
//
