LOCUS       sequence-424807-        7375 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses TALED_ Left-mouse-mtRnr1_A1555G-8e-E1347A in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7375)
  AUTHORS   Cho SI, Lim K, Hong S, Lee J, Kim A, Lim CJ, Ryou S, Lee JM, Mok YG,
            Chung E, Kim S, Han S, Cho SM, Kim J, Kim EK, Nam KH, Oh Y, Choi M, 
            An TH, Oh KJ, Lee S, Lee H, Kim JS
  TITLE     Engineering TALE-linked deaminases to facilitate precision adenine 
            base editing in mitochondrial DNA.
  JOURNAL   Cell. 2024 Jan 4;187(1):95-109.e26. doi: 10.1016/j.cell.2023.11.035.
  PUBMED    38181745
REFERENCE   2  (bases 1 to 7375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.11"; journalName: "Cell"; date: "2024-01-4- 4";
            volume: "187"; issue: "1"; pages: "95-109.e26"
COMMENT     Sequence Label: TALED_ Left-mouse-mtRnr1_A1555G-8e-E1347A
FEATURES             Location/Qualifiers
     source          1..7375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(2412..2430)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2413..2430)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2450..2467)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2456..2680
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    2911..3032
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2948..2967)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3002..3021
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3081..3097)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3081..3097)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3094..3116)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3105..3121
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3129..3135,3136..3153,3154..3159))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3174..3195
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3312..3329)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3483..4071)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3563..3582)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4242..5033,5034..5102))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4865..4884
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5103..5207)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5282..5301)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5473..5852
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5853..6056
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6007..6027
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6101..6120
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6101..6119
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6216..6242
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6246..6272
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6276..6302
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tcgtcgctat cgcaagtaat atcggcggga agcaagcttt ggaaacagtg caacgcttgt
       61 tgccagttct gtgccaagcc catggtctga caccagcaca agtggtcgcc atcgcatcca
      121 acggtggtgg gaaacaggca ttggagacag ttcaacggct gcttcctgtg ctgtgccagg
      181 ctcacggact gactcctgac caagttgttg caatcgccag caataatggt ggcaaacaag
      241 cccttgagac agtccagcgg ctcctccccg tcctttgtca agcacatggc ctcacaccag
      301 ctcaagtcgt tgctattgct tcacatgatg gaggtaaaca agcacttgaa accgtgcaaa
      361 gactcttgcc cgtgctttgt caagcacatg gcttgacacc cgaacaagtg gtcgctattg
      421 catccaacgg agggggcaag caggctcttg aaacagtgca acgcctgctg ccagtccttt
      481 gccaagccca tggccttact cctgaccaag tcgtggcaat tgctagtaac ggtggcggga
      541 aacaagcact tgaaactgtt cagcgcttgt tgcccgttct ctgtcaggat cacggtctta
      601 caccagatca ggttgtggcc attgcctcaa acataggagg aaaacaagca ttggagacag
      661 tgcaaaggct tttgccagtt ttgtgtcaag accatgggct gactcctgac caggtcgttg
      721 ccattgctag tcatgacggg ggtaaacagg ctctcgaaac tgtgcaacgc ttgttgccag
      781 ttttgtgcca agctcatggc ttgacacctg cacaagtggt tgcaatcgct tcacacgacg
      841 gaggaaagca agccctcgaa actgtgcagc ggctcctccc cgtgctgtgt caagatcacg
      901 gccttacacc cgctcaagtc gtcgcaattg ccagcaatgg aggcggtaag caggccttgg
      961 agactgttca gaggttgttg cccgtgcttt gccaagatca tgggcttaca cctgcacaag
     1021 ttgttgcaat tgcaagcaac ggcggcggaa aacaggcact ggaaactgtg caaagactcc
     1081 tgccagtttt gtgccaggct catggcctga ctccagacca ggtcgttgct attgccagca
     1141 ataacggagg taagcaagca ttggagactg ttcagcggct cctccctgtc ctctgtcagg
     1201 cccacgggct gactcctgca caggtggtgg ccatcgcttc aaacgggggg gggaagcaag
     1261 ccctcgaaac tgttcaacgg ttgttgcctg ttttgtgtca ggatcacggt ctgactccgg
     1321 aacaggtggt ggcgattgca agcaacggcg gcggcaaaca ggctctagag agcattgttg
     1381 cccagctctc cagacctgat ccggcgctag ccgcgttgct agtctcagga agcgaaactc
     1441 ctggtacctc agagtccgct actcccgaat cctctgaggt ggagttttcc cacgagtact
     1501 ggatgagaca tgccctgacc ctggccaaga gggcacggga tgagagggag gtgcctgtgg
     1561 gagccgtgct ggtgctgaac aatagagtga tcggcgaggg ctggaacaga gccatcggcc
     1621 tgcacgaccc aacagcccat gccgaaatta tggccctgag acagggcggc ctggtcatgc
     1681 agaactacag actgattgac gccaccctgt acgtgacatt cgagccttgc gtgatgtgcg
     1741 ccggcgccat gatccactct aggatcggcc gcgtggtgtt tggcgtgagg aactcaaaaa
     1801 gaggcgccgc aggctccctg atgaacgtgc tgaactaccc cggcatgaat caccgcgtcg
     1861 aaattaccga gggaatcctg gcagatgaat gtgccgccct gctgtgcgat ttctatcgga
     1921 tgcctagaca ggtgttcaat gctcagaaga aggcccagag ctccatcaac ctagtcggat
     1981 ccggcagcta cgccctgggt ccgtatcaga ttagcgcccc gcagctgcca gcttacaatg
     2041 gtcagaccgt gggtaccttc tactatgtga acgacgcggg cggtctggag agcaaggtgt
     2101 ttagcagcgg cggtccaacc ccgtacccaa actatgccaa tgccggtcat gtggccggtc
     2161 agagcgccct gttcatgcgt gataacggca tcagcgaggg tctggtgttc cacaacaacc
     2221 cggaaggcac ctgcggtttt tgcgtgaaca tgaccgaaac actgctgccg gaaaacgcga
     2281 aaatgaccgt ggtgccgccg gaaggtgcca ttccagtgaa gcgcggcgct accggtgaaa
     2341 ccaaagtgtt taccggtaac agcaacagcc cgaagagccc gaccaaaggc ggttgctagt
     2401 cagggcccta ttctatagtg tcacctaaat gctagagctc gctgatcagc ctcgactgtg
     2461 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     2521 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     2581 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     2641 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     2701 agctggggct ctagggggta tccccacgct gcagtgagcg ggactctggg gttcgaaatg
     2761 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     2821 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     2881 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     2941 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     3001 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     3061 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     3121 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     3181 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     3241 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     3301 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     3361 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     3421 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     3481 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     3541 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     3601 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     3661 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     3721 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     3781 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     3841 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     3901 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     3961 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     4021 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     4081 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     4141 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     4201 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     4261 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     4321 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     4381 cgagatccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     4441 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     4501 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     4561 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     4621 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     4681 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     4741 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     4801 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     4861 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     4921 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     4981 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     5041 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     5101 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     5161 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     5221 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca
     5281 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg
     5341 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
     5401 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca
     5461 gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
     5521 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     5581 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     5641 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     5701 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     5761 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     5821 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     5881 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     5941 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     6001 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctct
     6061 ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact cactataggg
     6121 agatggctct tagtcgagcc gtctgtggta cttcccgaca gcttgctcca gtcttggggt
     6181 accttggctc ccgacaaaag cactctctcc ctgattaccc ttacgatgta cctgactacg
     6241 ccggctaccc ttacgacgta ccagattacg ctggatatcc atacgatgtc cctgattatg
     6301 ctgggatccg aattcaagat ctacgcacgc tcggctacag ccagcagcaa caggagaaga
     6361 tcaaaccgaa ggttcgttcg acagtggcgc agcaccacga ggcactggtc ggccatgggt
     6421 ttacacacgc gcacatcgtt gcgctcagcc aacacccggc agcgttaggg accgtcgctg
     6481 tcaagtatca ggacatgatc gcagcgttgc cagaggcgac acacgaagcg atcgttggcg
     6541 tcggcaaaca gtggtccggc gcacgcgctc tggaggcctt gctcacggtg gcgggagagt
     6601 tgagaggtcc accgttacag ttggacacag gccaacttct caagattgca aaacgtggcg
     6661 gcgtgaccgc agtggaggca gtgcatgcat ggcgcaatgc actgacgggt gcccccctga
     6721 acctgacccc tgcccaggtg gtggccattg cctcccacga tggaggtaag caggctctgg
     6781 agacagttca aagattgctt ccagttcttt gccaggatca cggtttgaca cccgaccaag
     6841 tcgttgcaat cgccagtcat gatggtggta agcaagcact cgaaaccgtc cagcgcttgc
     6901 tgcccgtgct ctgccaggct catgggctga cacctgatca ggtcgtggcc atcgcatcaa
     6961 atataggggg taaacaagct ttggagactg tccagaggct cctccccgtc ttgtgtcaag
     7021 cccatgggtt gactcctgac caggtggttg ctatcgcttc aaacaatggt ggtaaacagg
     7081 ctctcgaaac cgtccagagg ttgttgcccg tgctttgcca ggcacacgga cttacccctg
     7141 cacaggtggt tgctattgcc tccaacggtg gaggcaaaca ggcccttgag actgtgcaac
     7201 gcctccttcc cgtgctgtgc caagatcatg ggctgacacc agcccaagtc gtcgctatcg
     7261 caagtaatat cggcgggaag caagctttgg aaacagtgca acgcttgttg ccagttctgt
     7321 gccaagccca tggtctgaca ccagcacaag tggtcgccat cgcatccaac ggtgg
//
