LOCUS       sequence-424812-        8705 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  1xGCN4pep transgene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8705)
  TITLE     Cheeseman lab plasmids Nov 2023
REFERENCE   2  (bases 1 to 8705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGS373_tdTomato-1xGCN4-T2A-BSD Lenti
FEATURES             Location/Qualifiers
     source          1..8705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    94..115
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(130..135,136..153,154..160)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    168..184
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     173..195
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     192..208
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     192..208
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     227..247
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        229..247
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        275..501
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             502..682
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    729..854
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1351..1584
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1769..1813
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     1991..2011
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    2212..2329
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2401..2612
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2639..2648
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2645..4072
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             4118..4138
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4151..4195
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             4232..4297
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4328..4381
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4388..4786
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    4832..5420
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4885..4905)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5303..5314)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5492..5725
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5803..5924
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5840..5859)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5894..5913
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5964..6099
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6119..6138)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6120..6138)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6148..6165)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6148..6164)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6157..6179)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6306..6761
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6393..6412)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6603..6624
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6787..6891
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6892..6960,6961..7752)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7110..7129)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7923..8511
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8412..8431
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8665..8682
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
       61 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
      121 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
      181 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
      241 ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag
      301 tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt
      361 gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg
      421 ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag
      481 tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc
      541 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
      601 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
      661 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
      721 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
      781 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
      841 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
      901 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
      961 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     1021 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     1081 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     1141 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc
     1201 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     1261 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     1321 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     1381 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     1441 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     1501 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     1561 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     1621 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     1681 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     1741 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     1801 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     1861 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     1921 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     1981 gggacccaga gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc
     2041 tgttagagag ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac
     2101 gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat
     2161 ggactatcat aaattctaga tcttgagaca aatggcagta ttcatccaca attttaaaag
     2221 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     2281 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     2341 cagggacagc agagatccac tttggcgccg gctcgtcgag tggctccggt gcccgtcagt
     2401 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2461 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2521 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2581 tttttcgcaa cgggtttgcc gccagaacac aggtgtcgtg acgcgggatc cgatatccgc
     2641 caccatggtg agcaagggcg aggaggtcat caaagagttc atgcgcttca aggtgcgcat
     2701 ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     2761 cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg
     2821 ggacatcctg tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc accccgccga
     2881 catccccgat tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     2941 cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg acggcacgct
     3001 gatctacaag gtgaagatgc gcggcaccaa cttccccccc gacggccccg taatgcagaa
     3061 gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa
     3121 gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa
     3181 gaccatctac atggccaaga agcccgtgca actgcccggc tactactacg tggacaccaa
     3241 gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg agcgctccga
     3301 gggccgccac cacctgttcc tggggcatgg caccggcagc accggcagcg gcagctccgg
     3361 caccgcctcc tccgaggaca acaacatggc cgtcatcaaa gagttcatgc gcttcaaggt
     3421 gcgcatggag ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg
     3481 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt
     3541 cgcctgggac atcctgtccc cccagttcat gtacggctcc aaggcgtacg tgaagcaccc
     3601 cgccgacatc cccgattaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     3661 gatgaacttc gaggacggcg gtctggtgac cgtgacccag gactcctccc tgcaggacgg
     3721 cacgctgatc tacaaggtga agatgcgcgg caccaacttc ccccccgacg gccccgtaat
     3781 gcagaagaag accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt
     3841 gctgaagggc gagatccacc aggccctgaa gctgaaggac ggcggccact acctggtgga
     3901 gttcaagacc atctacatgg ccaagaagcc cgtgcaactg cccggctact actacgtgga
     3961 caccaagctg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg
     4021 ctccgagggc cgccaccacc tgttcctgta cggcatggac gagctgtaca agaccggagg
     4081 ggatcggtgg agctccaccg gtggcggccg ctctagagag aatctttatt ttcagggcgc
     4141 cgccaaattc aaagaaaccg ctgctgctaa attcgaacgc cagcacatgg acagcggagg
     4201 tggaggtact agtctcgagg gttctggagg attgttgccc aaaaactacc acttggagaa
     4261 cgaggtcgca aggttgaaaa aattggtcgg tgagcgtggc ggctcaggcg gccttcttcc
     4321 tgaattcgag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc
     4381 tatcgatatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc
     4441 tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag
     4501 cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga
     4561 actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc
     4621 gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct
     4681 cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt
     4741 tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaaggta ccactagtcc
     4801 cgggatgcat agcgctacgc gttaagtcga caatcaacct ctggattaca aaatttgtga
     4861 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     4921 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     4981 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     5041 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     5101 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     5161 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     5221 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     5281 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     5341 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     5401 cctttgggcc gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat
     5461 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     5521 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     5581 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     5641 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     5701 tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag
     5761 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     5821 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     5881 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     5941 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     6001 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     6061 agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc
     6121 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     6181 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     6241 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     6301 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     6361 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     6421 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     6481 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     6541 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     6601 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     6661 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     6721 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     6781 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     6841 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     6901 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     6961 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     7021 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     7081 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     7141 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     7201 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     7261 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     7321 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     7381 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     7441 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     7501 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     7561 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     7621 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     7681 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     7741 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     7801 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     7861 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     7921 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     7981 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     8041 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     8101 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     8161 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     8221 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     8281 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     8341 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     8401 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     8461 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     8521 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     8581 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     8641 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     8701 atacg
//
