LOCUS       sequence-424814-        9116 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  6xGCN4pep transgene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9116)
  TITLE     Cheeseman lab plasmids Nov 2023
REFERENCE   2  (bases 1 to 9116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGS377_tdTomato-6xGCN4-T2A-BSD Lenti
FEATURES             Location/Qualifiers
     source          1..9116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    73..94
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(109..114,115..132,133..139)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    147..163
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     152..174
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     171..187
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     171..187
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     206..226
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        208..226
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        254..480
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             481..661
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    708..833
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1330..1563
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1748..1792
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     1970..1990
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    2191..2308
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2380..2591
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2618..2627
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2624..4051
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             4097..4117
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4130..4174
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             4211..4276
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4292..4357
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4373..4438
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4454..4519
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4535..4600
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4616..4681
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4718..4771
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4778..5176
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    5222..5810
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5275..5295)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5693..5704)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5882..6115
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6193..6314
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6230..6249)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6284..6303
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6354..6489
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6509..6528)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6510..6528)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6538..6555)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6538..6554)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6547..6569)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6696..7151
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6783..6802)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6993..7014
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7177..7281
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7282..7350,7351..8142)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7500..7519)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8313..8901
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8802..8821
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9055..9072
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
       61 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
      121 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
      181 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
      241 gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg
      301 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta
      361 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga
      421 accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac
      481 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
      541 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      601 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      661 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      721 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      781 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      841 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      901 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      961 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     1021 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     1081 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     1141 aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg
     1201 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     1261 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     1321 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     1381 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     1441 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     1501 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     1561 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     1621 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     1681 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     1741 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     1801 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     1861 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     1921 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccagag agggcctatt
     1981 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattagaat
     2041 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt
     2101 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata aattctagat
     2161 cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     2221 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     2281 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact
     2341 ttggcgccgg ctcgtcgagt ggctccggtg cccgtcagtg ggcagagcgc acatcgccca
     2401 cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc
     2461 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     2521 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     2581 ccagaacaca ggtgtcgtga cgcgggatcc gatatccgcc accatggtga gcaagggcga
     2641 ggaggtcatc aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca
     2701 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     2761 gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt
     2821 catgtacggc tccaaggcgt acgtgaagca ccccgccgac atccccgatt acaagaagct
     2881 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt
     2941 gaccgtgacc caggactcct ccctgcagga cggcacgctg atctacaagg tgaagatgcg
     3001 cggcaccaac ttcccccccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     3061 ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc accaggccct
     3121 gaagctgaag gacggcggcc actacctggt ggagttcaag accatctaca tggccaagaa
     3181 gcccgtgcaa ctgcccggct actactacgt ggacaccaag ctggacatca cctcccacaa
     3241 cgaggactac accatcgtgg aacagtacga gcgctccgag ggccgccacc acctgttcct
     3301 ggggcatggc accggcagca ccggcagcgg cagctccggc accgcctcct ccgaggacaa
     3361 caacatggcc gtcatcaaag agttcatgcg cttcaaggtg cgcatggagg gctccatgaa
     3421 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     3481 cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc
     3541 ccagttcatg tacggctcca aggcgtacgt gaagcacccc gccgacatcc ccgattacaa
     3601 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     3661 tctggtgacc gtgacccagg actcctccct gcaggacggc acgctgatct acaaggtgaa
     3721 gatgcgcggc accaacttcc cccccgacgg ccccgtaatg cagaagaaga ccatgggctg
     3781 ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg agatccacca
     3841 ggccctgaag ctgaaggacg gcggccacta cctggtggag ttcaagacca tctacatggc
     3901 caagaagccc gtgcaactgc ccggctacta ctacgtggac accaagctgg acatcacctc
     3961 ccacaacgag gactacacca tcgtggaaca gtacgagcgc tccgagggcc gccaccacct
     4021 gttcctgtac ggcatggacg agctgtacaa gaccggaggg gatcggtgga gctccaccgg
     4081 tggcggccgc tctagagaga atctttattt tcagggcgcc gccaaattca aagaaaccgc
     4141 tgctgctaaa ttcgaacgcc agcacatgga cagcggaggt ggaggtacta gtctcgaggg
     4201 ttctggagga ttgttgccca aaaactacca cttggagaac gaggtcgcaa ggttgaaaaa
     4261 attggtcggt gagcgtggcg gctcaggcgg ccttcttcca aaaaattatc atcttgaaaa
     4321 tgaagttgct agacttaaaa aacttgttgg agaaagaggc gggagcggcg gcctgctgcc
     4381 caagaactac cacctggaga acgaggtcgc ccgcctgaag aagctggtcg gcgagcgcgg
     4441 tggttctgga ggtttgctcc caaagaacta ccacttggaa aacgaagtcg caagattgaa
     4501 gaagttggtc ggtgaaagag gaggaagcgg aggattatta cctaaaaatt atcatttgga
     4561 aaatgaagtt gctagattga agaagttggt tggagagaga ggggggtctg gaggactact
     4621 accgaaaaac taccacctag agaacgaggt ggcccgacta aaaaagctag tgggcgagcg
     4681 cggcggcagt ggcggcctcc tccccaagaa tgaattcgag ggcagaggaa gtcttctaac
     4741 atgcggtgac gtggaggaga atcccggccc tatcgatatg gccaagcctt tgtctcaaga
     4801 agaatccacc ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga
     4861 ctacagcgtc gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt
     4921 atatcatttt actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc
     4981 ggcagctggc aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag
     5041 cccctgcgga cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt
     5101 gaaggacagt gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta
     5161 tgtgtgggag ggctaaggta ccactagtcc cgggatgcat agcgctacgc gttaagtcga
     5221 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     5281 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     5341 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     5401 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     5461 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     5521 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     5581 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     5641 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     5701 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     5761 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta
     5821 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     5881 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     5941 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     6001 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     6061 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg
     6121 tatagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     6181 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     6241 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     6301 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc
     6361 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     6421 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     6481 ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
     6541 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     6601 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     6661 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     6721 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     6781 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     6841 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     6901 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     6961 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     7021 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     7081 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     7141 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     7201 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     7261 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     7321 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     7381 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     7441 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     7501 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     7561 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     7621 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     7681 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     7741 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     7801 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     7861 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     7921 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     7981 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     8041 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     8101 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     8161 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     8221 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     8281 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     8341 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     8401 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     8461 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     8521 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     8581 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     8641 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     8701 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     8761 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     8821 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     8881 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     8941 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     9001 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     9061 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcg
//
