LOCUS       sequence-424815-        9437 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  10xGCN4pep transgene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9437)
  TITLE     Cheeseman lab plasmids Nov 2023
REFERENCE   2  (bases 1 to 9437)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGS379_tdTomato-10xGCN4-T2A-BSD Lenti
FEATURES             Location/Qualifiers
     source          1..9437
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    72..93
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(108..113,114..131,132..138)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    146..162
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     151..173
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     170..186
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     170..186
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     205..225
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        207..225
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        253..479
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             480..660
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    707..832
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1329..1562
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1747..1791
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     1969..1989
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    2190..2307
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2379..2590
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2617..2626
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2623..4050
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             4096..4116
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4129..4173
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             4210..4275
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4291..4356
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4372..4437
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4453..4518
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4534..4599
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4615..4680
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4696..4761
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4777..4842
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4858..4923
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4939..5004
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             5038..5091
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5098..5496
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    5542..6130
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5595..5615)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6013..6024)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6202..6435
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6513..6634
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6550..6569)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6604..6623
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6674..6809
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6829..6848)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6830..6848)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6858..6875)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6858..6874)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6867..6889)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7016..7471
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7103..7122)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7313..7334
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7497..7601
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7602..7670,7671..8462)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7820..7839)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8633..9221
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9122..9141
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9375..9392
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
       61 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
      121 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
      181 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
      241 ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga
      301 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa
      361 ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa
      421 ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg
      481 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
      541 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
      601 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
      661 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
      721 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
      781 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
      841 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
      901 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
      961 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     1021 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     1081 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     1141 aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga
     1201 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     1261 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     1321 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     1381 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     1441 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     1501 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     1561 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     1621 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     1681 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     1741 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     1801 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     1861 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     1921 tcaccattat cgtttcagac ccacctccca accccgaggg gacccagaga gggcctattt
     1981 cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat aattagaatt
     2041 aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt
     2101 cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcataa attctagatc
     2161 ttgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     2221 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     2281 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt
     2341 tggcgccggc tcgtcgagtg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     2401 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     2461 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     2521 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     2581 cagaacacag gtgtcgtgac gcgggatccg atatccgcca ccatggtgag caagggcgag
     2641 gaggtcatca aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac
     2701 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     2761 ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc
     2821 atgtacggct ccaaggcgta cgtgaagcac cccgccgaca tccccgatta caagaagctg
     2881 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg
     2941 accgtgaccc aggactcctc cctgcaggac ggcacgctga tctacaaggt gaagatgcgc
     3001 ggcaccaact tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     3061 tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg
     3121 aagctgaagg acggcggcca ctacctggtg gagttcaaga ccatctacat ggccaagaag
     3181 cccgtgcaac tgcccggcta ctactacgtg gacaccaagc tggacatcac ctcccacaac
     3241 gaggactaca ccatcgtgga acagtacgag cgctccgagg gccgccacca cctgttcctg
     3301 gggcatggca ccggcagcac cggcagcggc agctccggca ccgcctcctc cgaggacaac
     3361 aacatggccg tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac
     3421 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     3481 gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc
     3541 cagttcatgt acggctccaa ggcgtacgtg aagcaccccg ccgacatccc cgattacaag
     3601 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt
     3661 ctggtgaccg tgacccagga ctcctccctg caggacggca cgctgatcta caaggtgaag
     3721 atgcgcggca ccaacttccc ccccgacggc cccgtaatgc agaagaagac catgggctgg
     3781 gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccaccag
     3841 gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagaccat ctacatggcc
     3901 aagaagcccg tgcaactgcc cggctactac tacgtggaca ccaagctgga catcacctcc
     3961 cacaacgagg actacaccat cgtggaacag tacgagcgct ccgagggccg ccaccacctg
     4021 ttcctgtacg gcatggacga gctgtacaag accggagggg atcggtggag ctccaccggt
     4081 ggcggccgct ctagagagaa tctttatttt cagggcgccg ccaaattcaa agaaaccgct
     4141 gctgctaaat tcgaacgcca gcacatggac agcggaggtg gaggtactag tctcgagggt
     4201 tctggaggat tgttgcccaa aaactaccac ttggagaacg aggtcgcaag gttgaaaaaa
     4261 ttggtcggtg agcgtggcgg ctcaggcggc cttcttccaa aaaattatca tcttgaaaat
     4321 gaagttgcta gacttaaaaa acttgttgga gaaagaggcg ggagcggcgg cctgctgccc
     4381 aagaactacc acctggagaa cgaggtcgcc cgcctgaaga agctggtcgg cgagcgcggt
     4441 ggttctggag gtttgctccc aaagaactac cacttggaaa acgaagtcgc aagattgaag
     4501 aagttggtcg gtgaaagagg aggaagcgga ggattattac ctaaaaatta tcatttggaa
     4561 aatgaagttg ctagattgaa gaagttggtt ggagagagag gggggtctgg aggactacta
     4621 ccgaaaaact accacctaga gaacgaggtg gcccgactaa aaaagctagt gggcgagcgc
     4681 ggcggcagtg gcggcctcct ccccaagaat taccacctcg aaaatgaagt agcaaggctc
     4741 aagaagctcg taggagaaag aggtggttcc gggggactcc ttccaaaaaa ctatcatctt
     4801 gaaaacgaag ttgcacgtct taaaaaactt gttggcgaac gtggcggctc tggcgggctg
     4861 ctgcccaaga attaccacct ggaaaatgaa gtggcccgcc tgaagaagct ggtgggcgaa
     4921 cgcggaggca gtggaggcct actaccgaag aactaccacc tagagaacga ggtggcacgc
     4981 ctaaagaagc tagtgggcga gcgcggcggc tcgggtggtt tattacctaa ggaattcgag
     5041 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc tatcgatatg
     5101 gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac
     5161 agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag cgacggccgc
     5221 atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga actcgtggtg
     5281 ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat
     5341 gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct cgatctgcat
     5401 cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt
     5461 gaattgctgc cctctggtta tgtgtgggag ggctaaggta ccactagtcc cgggatgcat
     5521 agcgctacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     5581 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     5641 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     5701 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     5761 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     5821 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     5881 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     5941 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     6001 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     6061 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     6121 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
     6181 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     6241 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     6301 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     6361 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     6421 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
     6481 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     6541 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     6601 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     6661 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     6721 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     6781 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     6841 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     6901 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     6961 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     7021 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     7081 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     7141 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     7201 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     7261 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     7321 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     7381 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     7441 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     7501 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     7561 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     7621 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     7681 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     7741 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     7801 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     7861 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     7921 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     7981 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     8041 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     8101 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     8161 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     8221 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     8281 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     8341 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     8401 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     8461 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     8521 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     8581 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     8641 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     8701 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     8761 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     8821 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     8881 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     8941 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     9001 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     9061 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     9121 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     9181 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     9241 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     9301 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     9361 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     9421 cgcctctccc cgcgcgt
//
