LOCUS       sequence-424816-        9590 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  12xGCN4pep transgene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9590)
  TITLE     Cheeseman lab plasmids Nov 2023
REFERENCE   2  (bases 1 to 9590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGS380_tdTomato-12xGCN4-T2A-BSD Lenti
FEATURES             Location/Qualifiers
     source          1..9590
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    73..94
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(109..114,115..132,133..139)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    147..163
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     152..174
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     171..187
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     171..187
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     206..226
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        208..226
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        254..480
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             481..661
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    708..833
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1330..1563
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1748..1792
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     1970..1990
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    2191..2308
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2380..2591
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2618..2627
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2624..4051
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             4097..4117
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4130..4174
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             4211..4276
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4292..4357
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4373..4438
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4454..4519
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4535..4600
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4616..4681
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4697..4762
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4778..4843
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4859..4924
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4940..5005
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             5021..5086
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             5102..5167
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             5192..5245
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5252..5650
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    5696..6284
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5749..5769)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6167..6178)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6356..6589
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6667..6788
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6704..6723)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6758..6777
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6828..6963
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6983..7002)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6984..7002)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7012..7029)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7012..7028)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7021..7043)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7170..7625
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7257..7276)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7467..7488
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7651..7755
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7756..7824,7825..8616)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7974..7993)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8787..9375
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9276..9295
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9529..9546
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
       61 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
      121 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
      181 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
      241 gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg
      301 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta
      361 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga
      421 accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac
      481 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
      541 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      601 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      661 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      721 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      781 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      841 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      901 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      961 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     1021 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     1081 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     1141 aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg
     1201 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     1261 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     1321 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     1381 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     1441 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     1501 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     1561 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     1621 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     1681 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     1741 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     1801 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     1861 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     1921 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccagag agggcctatt
     1981 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattagaat
     2041 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt
     2101 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata aattctagat
     2161 cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     2221 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     2281 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact
     2341 ttggcgccgg ctcgtcgagt ggctccggtg cccgtcagtg ggcagagcgc acatcgccca
     2401 cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc
     2461 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     2521 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     2581 ccagaacaca ggtgtcgtga cgcgggatcc gatatccgcc accatggtga gcaagggcga
     2641 ggaggtcatc aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca
     2701 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     2761 gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt
     2821 catgtacggc tccaaggcgt acgtgaagca ccccgccgac atccccgatt acaagaagct
     2881 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt
     2941 gaccgtgacc caggactcct ccctgcagga cggcacgctg atctacaagg tgaagatgcg
     3001 cggcaccaac ttcccccccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     3061 ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc accaggccct
     3121 gaagctgaag gacggcggcc actacctggt ggagttcaag accatctaca tggccaagaa
     3181 gcccgtgcaa ctgcccggct actactacgt ggacaccaag ctggacatca cctcccacaa
     3241 cgaggactac accatcgtgg aacagtacga gcgctccgag ggccgccacc acctgttcct
     3301 ggggcatggc accggcagca ccggcagcgg cagctccggc accgcctcct ccgaggacaa
     3361 caacatggcc gtcatcaaag agttcatgcg cttcaaggtg cgcatggagg gctccatgaa
     3421 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     3481 cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc
     3541 ccagttcatg tacggctcca aggcgtacgt gaagcacccc gccgacatcc ccgattacaa
     3601 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     3661 tctggtgacc gtgacccagg actcctccct gcaggacggc acgctgatct acaaggtgaa
     3721 gatgcgcggc accaacttcc cccccgacgg ccccgtaatg cagaagaaga ccatgggctg
     3781 ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg agatccacca
     3841 ggccctgaag ctgaaggacg gcggccacta cctggtggag ttcaagacca tctacatggc
     3901 caagaagccc gtgcaactgc ccggctacta ctacgtggac accaagctgg acatcacctc
     3961 ccacaacgag gactacacca tcgtggaaca gtacgagcgc tccgagggcc gccaccacct
     4021 gttcctgtac ggcatggacg agctgtacaa gaccggaggg gatcggtgga gctccaccgg
     4081 tggcggccgc tctagagaga atctttattt tcagggcgcc gccaaattca aagaaaccgc
     4141 tgctgctaaa ttcgaacgcc agcacatgga cagcggaggt ggaggtacta gtctcgaggg
     4201 ttctggagga ttgttgccca aaaactacca cttggagaac gaggtcgcaa ggttgaaaaa
     4261 attggtcggt gagcgtggcg gctcaggcgg ccttcttcca aaaaattatc atcttgaaaa
     4321 tgaagttgct agacttaaaa aacttgttgg agaaagaggc gggagcggcg gcctgctgcc
     4381 caagaactac cacctggaga acgaggtcgc ccgcctgaag aagctggtcg gcgagcgcgg
     4441 tggttctgga ggtttgctcc caaagaacta ccacttggaa aacgaagtcg caagattgaa
     4501 gaagttggtc ggtgaaagag gaggaagcgg aggattatta cctaaaaatt atcatttgga
     4561 aaatgaagtt gctagattga agaagttggt tggagagaga ggggggtctg gaggactact
     4621 accgaaaaac taccacctag agaacgaggt ggcccgacta aaaaagctag tgggcgagcg
     4681 cggcggcagt ggcggcctcc tccccaagaa ttaccacctc gaaaatgaag tagcaaggct
     4741 caagaagctc gtaggagaaa gaggtggttc cgggggactc cttccaaaaa actatcatct
     4801 tgaaaacgaa gttgcacgtc ttaaaaaact tgttggcgaa cgtggcggct ctggcgggct
     4861 gctgcccaag aattaccacc tggaaaatga agtggcccgc ctgaagaagc tggtgggcga
     4921 acgcggaggc agtggaggcc tactaccgaa gaactaccac ctagagaacg aggtggcacg
     4981 cctaaagaag ctagtgggcg agcgcggcgg ctcgggtggt ttattaccta agaattatca
     5041 tttagaaaat gaagtagctc gattaaagaa gttagtaggt gaacgtggtg ggtctggtgg
     5101 tcttcttccg aagaactacc accttgagaa cgaggtagct cgtcttaaga aacttgtagg
     5161 tgagcgtggt ggaagcggtg gtggggaatt cgagggcaga ggaagtcttc taacatgcgg
     5221 tgacgtggag gagaatcccg gccctatcga tatggccaag cctttgtctc aagaagaatc
     5281 caccctcatt gaaagagcaa cggctacaat caacagcatc cccatctctg aagactacag
     5341 cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc actggtgtca atgtatatca
     5401 ttttactggg ggaccttgtg cagaactcgt ggtgctgggc actgctgctg ctgcggcagc
     5461 tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac aggggcatct tgagcccctg
     5521 cggacggtgc cgacaggtgc ttctcgatct gcatcctggg atcaaagcca tagtgaagga
     5581 cagtgatgga cagccgacgg cagttgggat tcgtgaattg ctgccctctg gttatgtgtg
     5641 ggagggctaa ggtaccacta gtcccgggat gcatagcgct acgcgttaag tcgacaatca
     5701 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     5761 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     5821 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     5881 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     5941 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     6001 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     6061 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     6121 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     6181 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     6241 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca
     6301 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     6361 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     6421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     6481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     6541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt
     6601 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     6661 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     6721 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     6781 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     6841 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     6901 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     6961 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     7021 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     7081 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     7141 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     7201 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     7261 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     7321 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     7381 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     7441 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     7501 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     7561 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     7621 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     7681 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     7741 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     7801 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     7861 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     7921 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     7981 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     8041 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     8101 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     8161 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     8221 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     8281 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     8341 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     8401 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     8461 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     8521 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     8581 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     8641 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     8701 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     8761 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     8821 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     8881 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     8941 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     9001 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     9061 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     9121 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     9181 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     9241 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     9301 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     9361 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     9421 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     9481 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     9541 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
//
