LOCUS       sequence-424817-        9809 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  14xGCN4pep transgene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9809)
  TITLE     Cheeseman lab plasmids Nov 2023
REFERENCE   2  (bases 1 to 9809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGS381_tdTomato-14xGCN4-T2A-BSD Lenti
FEATURES             Location/Qualifiers
     source          1..9809
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    56..77
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(92..97,98..115,116..122)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    130..146
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     135..157
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     154..170
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     154..170
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     189..209
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        191..209
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        237..463
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             464..644
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    691..816
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1313..1546
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1731..1775
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     1953..1973
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    2174..2291
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2363..2574
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2601..2610
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2607..4034
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             4080..4100
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4113..4157
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             4194..4259
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4275..4340
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4356..4421
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4437..4502
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4518..4583
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4599..4664
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4680..4745
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4761..4826
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4842..4907
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             4923..4988
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             5004..5069
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             5085..5150
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     primer_bind     5166..5188
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     primer_bind     5189..5211
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             5214..5279
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             5295..5360
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             5394..5447
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5454..5852
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    5898..6486
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5951..5971)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6369..6380)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6558..6791
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6869..6990
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6906..6925)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6960..6979
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7030..7165
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7185..7204)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7186..7204)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7214..7231)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7214..7230)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7223..7245)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7372..7827
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7459..7478)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7669..7690
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7853..7957
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7958..8026,8027..8818)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8176..8195)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8989..9577
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9478..9497
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9731..9748
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
       61 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
      121 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
      181 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta
      241 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt
      301 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc
      361 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca
      421 ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga
      481 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
      541 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
      601 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
      661 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
      721 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
      781 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
      841 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
      901 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
      961 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     1021 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     1081 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     1141 accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     1201 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1261 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1321 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1381 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1441 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1501 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1561 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1621 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1681 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1741 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1801 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1861 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1921 agacccacct cccaaccccg aggggaccca gagagggcct atttcccatg attccttcat
     1981 atttgcatat acgatacaag gctgttagag agataattag aattaatttg actgtaaaca
     2041 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
     2101 ttttaaaatt atgttttaaa atggactatc ataaattcta gatcttgaga caaatggcag
     2161 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     2221 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     2281 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgtcg
     2341 agtggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     2401 gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa
     2461 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     2521 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtgtcg
     2581 tgacgcggga tccgatatcc gccaccatgg tgagcaaggg cgaggaggtc atcaaagagt
     2641 tcatgcgctt caaggtgcgc atggagggct ccatgaacgg ccacgagttc gagatcgagg
     2701 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     2761 gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttcatgtac ggctccaagg
     2821 cgtacgtgaa gcaccccgcc gacatccccg attacaagaa gctgtccttc cccgagggct
     2881 tcaagtggga gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg acccaggact
     2941 cctccctgca ggacggcacg ctgatctaca aggtgaagat gcgcggcacc aacttccccc
     3001 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccacc gagcgcctgt
     3061 acccccgcga cggcgtgctg aagggcgaga tccaccaggc cctgaagctg aaggacggcg
     3121 gccactacct ggtggagttc aagaccatct acatggccaa gaagcccgtg caactgcccg
     3181 gctactacta cgtggacacc aagctggaca tcacctccca caacgaggac tacaccatcg
     3241 tggaacagta cgagcgctcc gagggccgcc accacctgtt cctggggcat ggcaccggca
     3301 gcaccggcag cggcagctcc ggcaccgcct cctccgagga caacaacatg gccgtcatca
     3361 aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga
     3421 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
     3481 ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct
     3541 ccaaggcgta cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg
     3601 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc
     3661 aggactcctc cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact
     3721 tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc
     3781 gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg
     3841 acggcggcca ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac
     3901 tgcccggcta ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca
     3961 ccatcgtgga acagtacgag cgctccgagg gccgccacca cctgttcctg tacggcatgg
     4021 acgagctgta caagaccgga ggggatcggt ggagctccac cggtggcggc cgctctagag
     4081 agaatcttta ttttcagggc gccgccaaat tcaaagaaac cgctgctgct aaattcgaac
     4141 gccagcacat ggacagcgga ggtggaggta ctagtctcga gggttctgga ggattgttgc
     4201 ccaaaaacta ccacttggag aacgaggtcg caaggttgaa aaaattggtc ggtgagcgtg
     4261 gcggctcagg cggccttctt ccaaaaaatt atcatcttga aaatgaagtt gctagactta
     4321 aaaaacttgt tggagaaaga ggcgggagcg gcggcctgct gcccaagaac taccacctgg
     4381 agaacgaggt cgcccgcctg aagaagctgg tcggcgagcg cggtggttct ggaggtttgc
     4441 tcccaaagaa ctaccacttg gaaaacgaag tcgcaagatt gaagaagttg gtcggtgaaa
     4501 gaggaggaag cggaggatta ttacctaaaa attatcattt ggaaaatgaa gttgctagat
     4561 tgaagaagtt ggttggagag agaggggggt ctggaggact actaccgaaa aactaccacc
     4621 tagagaacga ggtggcccga ctaaaaaagc tagtgggcga gcgcggcggc agtggcggcc
     4681 tcctccccaa gaattaccac ctcgaaaatg aagtagcaag gctcaagaag ctcgtaggag
     4741 aaagaggtgg ttccggggga ctccttccaa aaaactatca tcttgaaaac gaagttgcac
     4801 gtcttaaaaa acttgttggc gaacgtggcg gctctggcgg gctgctgccc aagaattacc
     4861 acctggaaaa tgaagtggcc cgcctgaaga agctggtggg cgaacgcgga ggcagtggag
     4921 gcctactacc gaagaactac cacctagaga acgaggtggc acgcctaaag aagctagtgg
     4981 gcgagcgcgg cggctcgggt ggtttattac ctaagaatta tcatttagaa aatgaagtag
     5041 ctcgattaaa gaagttagta ggtgaacgtg gtgggtctgg tggtcttctt ccgaagaact
     5101 accaccttga gaacgaggta gctcgtctta agaaacttgt aggtgagcgt ggtggaagcg
     5161 gtggtgggct ggcaagccac gtttggtgcc gggagctgca tgtgtcagag gaacttttgc
     5221 caaagaatta tcatcttgag aacgaagtgg ctcgtcttaa gaaactcgta ggagaaagag
     5281 gtggcagtgg aggtctgctt cccaagaatt accacctgga aaatgaggta gctagactga
     5341 aaaagctcgt aggcgagcgc gggggaagtg ggggattgct gccaaaagaa ttcgagggca
     5401 gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatc gatatggcca
     5461 agcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca
     5521 tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct
     5581 tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg
     5641 gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga
     5701 acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg
     5761 ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat
     5821 tgctgccctc tggttatgtg tgggagggct aaggtaccac tagtcccggg atgcatagcg
     5881 ctacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     5941 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     6001 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     6061 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     6121 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     6181 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     6241 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     6301 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     6361 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     6421 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     6481 ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta gccacttttt
     6541 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     6601 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     6661 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     6721 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     6781 aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt tataacttgc
     6841 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     6901 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     6961 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     7021 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     7081 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     7141 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt
     7201 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     7261 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     7321 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
     7381 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     7441 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     7501 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     7561 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     7621 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     7681 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     7741 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     7801 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     7861 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     7921 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     7981 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     8041 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     8101 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     8161 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     8221 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     8281 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     8341 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     8401 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     8461 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     8521 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     8581 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     8641 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     8701 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     8761 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     8821 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     8881 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     8941 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     9001 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     9061 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     9121 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     9181 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     9241 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     9301 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     9361 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     9421 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     9481 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     9541 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     9601 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     9661 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     9721 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     9781 tctccccgcg cgttggccga ttcattaat
//
