LOCUS       sequence-424830-        7864 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  8xGCN4pep transgene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7864)
  TITLE     Cheeseman lab plasmids Nov 2023
REFERENCE   2  (bases 1 to 7864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGS400_myc-8xGCN4-T2A-BSD Lenti
FEATURES             Location/Qualifiers
     source          1..7864
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    88..109
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(124..129,130..147,148..154)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    162..178
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     167..189
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     186..202
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     186..202
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     221..241
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        223..241
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        269..495
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             496..676
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    723..848
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1345..1578
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1763..1807
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     1985..2005
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    2206..2323
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2395..2606
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2635..2664
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2707..2727
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2740..2784
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             2821..2886
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             2902..2967
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             2983..3048
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3064..3129
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3145..3210
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3226..3291
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3307..3372
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3388..3453
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             3481..3534
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3541..3939
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    3985..4573
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4038..4058)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4456..4467)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4645..4878
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4956..5077
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4993..5012)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5047..5066
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5117..5252
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5272..5291)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5273..5291)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5301..5318)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5301..5317)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5310..5332)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5459..5914
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5546..5565)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5756..5777
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5940..6044
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6045..6113,6114..6905)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6263..6282)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7076..7664
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7565..7584
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7818..7835
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
       61 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
      121 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
      181 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
      241 ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc
      301 aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc
      361 cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg
      421 acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta
      481 gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc
      541 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
      601 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
      661 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
      721 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
      781 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
      841 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
      901 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
      961 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     1021 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     1081 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     1141 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat
     1201 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     1261 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     1321 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     1381 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     1441 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     1501 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     1561 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     1621 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     1681 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     1741 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     1801 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     1861 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     1921 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     1981 cagagagggc ctatttccca tgattccttc atatttgcat atacgataca aggctgttag
     2041 agagataatt agaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg
     2101 tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta
     2161 tcataaattc tagatcttga gacaaatggc agtattcatc cacaatttta aaagaaaagg
     2221 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2281 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2341 cagcagagat ccactttggc gccggctcgt cgagtggctc cggtgcccgt cagtgggcag
     2401 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
     2461 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
     2521 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
     2581 gcaacgggtt tgccgccaga acacaggtgt cgtgacgcgg gatccgatat catggagcag
     2641 aagctgatca gcgaggagga cctgggaggg gatcggtgga gctccaccgg tggcggccgc
     2701 tctagagaga atctttattt tcagggcgcc gccaaattca aagaaaccgc tgctgctaaa
     2761 ttcgaacgcc agcacatgga cagcggaggt ggaggtacta gtctcgaggg ttctggagga
     2821 ttgttgccca aaaactacca cttggagaac gaggtcgcaa ggttgaaaaa attggtcggt
     2881 gagcgtggcg gctcaggcgg ccttcttcca aaaaattatc atcttgaaaa tgaagttgct
     2941 agacttaaaa aacttgttgg agaaagaggc gggagcggcg gcctgctgcc caagaactac
     3001 cacctggaga acgaggtcgc ccgcctgaag aagctggtcg gcgagcgcgg tggttctgga
     3061 ggtttgctcc caaagaacta ccacttggaa aacgaagtcg caagattgaa gaagttggtc
     3121 ggtgaaagag gaggaagcgg aggattatta cctaaaaatt atcatttgga aaatgaagtt
     3181 gctagattga agaagttggt tggagagaga ggggggtctg gaggactact accgaaaaac
     3241 taccacctag agaacgaggt ggcccgacta aaaaagctag tgggcgagcg cggcggcagt
     3301 ggcggcctcc tccccaagaa ttaccacctc gaaaatgaag tagcaaggct caagaagctc
     3361 gtaggagaaa gaggtggttc cgggggactc cttccaaaaa actatcatct tgaaaacgaa
     3421 gttgcacgtc ttaaaaaact tgttggcgaa cgtggcggct ctggcgggct gctggaattc
     3481 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatcgat
     3541 atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc
     3601 aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc
     3661 cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg
     3721 gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga
     3781 aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg
     3841 catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt
     3901 cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag gtaccactag tcccgggatg
     3961 catagcgcta cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     4021 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     4081 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     4141 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     4201 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     4261 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     4321 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     4381 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     4441 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     4501 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     4561 gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     4621 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     4681 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     4741 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     4801 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     4861 tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat
     4921 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     4981 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     5041 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     5101 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     5161 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     5221 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     5281 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     5341 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     5401 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     5461 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     5521 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     5581 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     5641 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     5701 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     5761 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     5821 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     5881 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     5941 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     6001 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     6061 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     6121 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     6181 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     6241 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     6301 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     6361 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     6421 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     6481 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     6541 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     6601 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     6661 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     6721 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     6781 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     6841 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     6901 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     6961 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     7021 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     7081 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     7141 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     7201 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     7261 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     7321 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     7381 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     7441 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     7501 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     7561 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     7621 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     7681 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     7741 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     7801 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     7861 aacc
//
