LOCUS       sequence-424844-         603 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of CREB1 domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Martinez-Yamout MA, Nasir I, Shnitkind S, Ellis JP, Berlow RB, Kroon
            G, Deniz AA, Dyson HJ, Wright PE
  TITLE     Glutamine-rich regions of the disordered CREB transactivation domain
            mediate dynamic intra- and intermolecular interactions.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Nov 21;120(47):e2313835120. doi: 
            10.1073/pnas.2313835120. Epub 2023 Nov 14.
  PUBMED    37971402
REFERENCE   2  (bases 1 to 603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-11-21"; volume: "120(47):e2313835120. 
            doi"; pages: " 10.1073/pnas.2313835120. Epub 2023 Nov 14"
COMMENT     Sequence Label: H6Gb1-CREB(85-160)
FEATURES             Location/Qualifiers
     source          1..603
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..34
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             80..241
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             266..286
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             566..583
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccatc atcatcatca
       61 tcacagcagc ggcatgactt acaaactgat ccttaacggt aaaactttga aaggcgaaac
      121 cactaccgaa gctgttgatg ctgcgactgc agaaaaagtt ttcaaacagt acgctaacga
      181 caacggtgtt gacggtgaat ggacttacga cgatgcgact aagaccttca ccgttactga
      241 agaattcagc agcggcagca gcggcgaaaa cctgtatttt cagggcacag ttcagtcttc
      301 ctgcaaggac ttaaaaagac ttttctccgg aactcagatt tcaactattg cagaaagtga
      361 agattcacag gagtctgtgg atagtgtaac tgattcccaa aaacgaaggg aaatcctttc
      421 aaggaggcct tcctacagga aaattttgaa tgacttatct tctgatgcac caggggtgcc
      481 aaggattgaa gaagaaaaat cagaagaaga gacttaatag gatccgaatt cgagctccgt
      541 cgacaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa
      601 caa
//
