LOCUS       sequence-424865-        9964 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Hfl1-8Flag. Uses auxotrophic marker LEU2(Saccharomyces
            cerevisiae). .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9964)
  AUTHORS   He CW, Cui XF, Ma SJ, Xu Q, Ran YP, Chen WZ, Mu JX, Li H, Zhu J, 
            Gong Q, Xie Z
  TITLE     Membrane recruitment of Atg8 by Hfl1 facilitates turnover of 
            vacuolar membrane proteins in yeast cells approaching stationary 
            phase.
  JOURNAL   BMC Biol. 2021 Jun 4;19(1):117. doi: 10.1186/s12915-021-01048-7.
  PUBMED    34088313
REFERENCE   2  (bases 1 to 9964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2021-06-4"; volume: "19(1):117. doi"; pages: " 
            10.1186/s12915-021-01048-7"
COMMENT     Sequence Label: P1K-HFL1-8flag-LEU
FEATURES             Location/Qualifiers
     source          1..9964
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        276..683
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             4805..4828
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4835..4858
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4865..4888
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4895..4918
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4925..4948
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4955..4978
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4985..5008
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5015..5038
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      5068..5233
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5123..5147)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(6511..6530)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6512..6530)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6537..6554)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6537..6553)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6546..6568)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6694..7149
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6781..6800)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6991..7012
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7505..7524)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7624..7646
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7684..7702)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7787..7891
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7892..7960,7961..8752)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8110..8129)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8923..9511
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9412..9431
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9665..9682
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9799..9820
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9835..9840,9841..9858,9859..9865)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9873..9889
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9878..9900
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9897..9913
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9897..9913
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        9934..9952
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggccggccat ttaaatgttt aaacggccac gctggccgag gagaacttct agtatatcta
       61 catacctaat attattgcct tattaaaaat ggaatcccaa cgattacatc aaaatccaca
      121 ttctcttcaa aatcaattgt cctgtacttc cttgttcatg tgtgttcaaa aacgttatat
      181 ttataggata attatactct atttctcaac aagtaattgg ttgtttggcc gagcggtcta
      241 aggcgcctga ttcaagaaat atcttgaccg cagttaactg tgggaatact caggtatcgt
      301 aagatgcaag agttcgaatc tcttagcaac cattattttt ttcctcaaca taacgagaac
      361 acacaggggc gctatcgcac agaatcaaat tcgatgactg gaaatttttt gttaatttca
      421 gaggtcgcct gacgcatata cctttttcaa ctgaaaaatt gggagaaaaa ggaaaggtga
      481 gagcgccgga accggctttt catatagaat agagaagcgt tcatgactaa atgcttgcat
      541 cacaatactt gaagttgaca atattattta aggacctatt gttttttcca ataggtggtt
      601 agcaatcgtc ttactttcta acttttctta ccttttacat ttcagcaata tatatatata
      661 tatttcaagg atataccatt ctaatgtctg cccctaagtt accaatagat agcggtttaa
      721 tataaggttt caacaagaaa tattttgtga aaatcaaaag ttaatagttt cactgtcgaa
      781 aatatgaaag tttaagtttt cagttcatcc cattgtagtt atcaaatttc tgtgtttccc
      841 gtatatgtgt gctctgtatt catcgctatc ttgaaactgg ttatgatcgt tgcgcatgtg
      901 acctgtatcg ctcaaaagag cccggcaccg gtaccgggca aaagccccta gcaagctaaa
      961 atgattcaac tttcgtagta atttgttcaa ccggtcacag taaagtcaat gactaacttt
     1021 tgaatcgagg atgtatttcc aattgaaagc aatgtatata taaaccattg tgttgaagct
     1081 tgtcttacag ttttcttgta tcctcccatt gttcatatag tacttaaagt accataatcc
     1141 ttaaagaaca aacttgaaag agaatacact atacacaact aataaataaa gaataatcat
     1201 ggctgtgacc aagacaattg tagttctacc aggtgaccat gttggtcaag aaatcactga
     1261 agaggccatt aaagttttaa atgctattca ggaatgtcgt ccagacaagg tcaatttcaa
     1321 gtttgatcat catttgatcg gtggtgctgc aattgatgcc actggtgttc cattaccaga
     1381 cgaagctttg gaggcctcta agaaagctga tgccgtgctt ctgggtgctg ttggtggtcc
     1441 aaaatggggt actggcgctg tcagaccaga acaaggtctt ttgaaaatcc gtaaggagtt
     1501 gcaattgtat gctaatctaa gaccatgtaa ttttgcatct gattccttac tagatctatc
     1561 gccattgaag cctgaaattg caagaggtac agatttcgtc gttgttagag aactagtggg
     1621 tggtatttat ttcggggaga gaaaagaaga tgaaggtgat ggtgtcgcct gggatagcga
     1681 aaagtattct gtgcccgaag ttcaaagaat cactagaatg gctgctttca tggctctgca
     1741 acacaaccca cctttaccta tttggtctct agataaagcc aacgttctag catcatcaag
     1801 attatggaga aaaacagtgg aagagacgat taagaatgaa ttcccacaat tgactgttca
     1861 acatcaattg attgattccg ctgctatgat cttggttaag aacccaactc atctaaatgg
     1921 tattattatc acaaacaata tgtttggtga tattatctcc gatgaagcct ccgtgatccc
     1981 aggttcgtta ggattacttc catctgcctc cttggcatct cttccagata agaatacggc
     2041 atttggttta tatgagccat gtcatggctc tgctccagac ttaccaaagg gaaaggttaa
     2101 ccccgtagca acaatcctat ctgctgctat gatgttgaag ttgtctctgg acctgttcga
     2161 agaaggtgaa attatagaac aagcagtcaa gaaagtgttg gattctggta tcagaacagc
     2221 ggatctaaag ggtaccaact ctactactga ggtcggtgat gcagtagcga aagctgtcag
     2281 ggaactatta gcttagactt agtagatgta cagttgcata cataagtctg aacagaaaga
     2341 agtaaatatc atatctagtt cggtttagaa atcagtgact atattggttt tgaccagatc
     2401 gcaatccttg caagcattta gtaatgacta tcaaaccggt gaatcatctg ataaggctat
     2461 attagcttgt gcattcgcat gtatcgggaa acgaacggta ccgggcccac catgatggcg
     2521 aatccgaaga gcgagacact gtatctacaa agtccaagcc gaagaaacag tccccaaaat
     2581 cgtttgttgg tgctcatttg aggaggcaac tctcttttga tttatggtac ctattccttg
     2641 gattatttat aatatgcata tgcgagggca gaaaaatcga agacgttaat aaacctgatt
     2701 tcaatgtctt tgctatattg tttgaagttg ttagcgctta tggtacagtg ggtttgtcat
     2761 tgggttaccc aaacaccaac acatcactat ctgcccagtt caccgtatta tcgaagctag
     2821 tcataattgc catgctaata agaggaagaa atagaggttt accatacact ttggatcgtg
     2881 ccatcatgct gccaagtgac aaactggaac aaattgatcg tttacaagat atgaaagcta
     2941 agggtaagtt gttagccaaa gttggtgagg atccaatgac tacttacgtc aaaaagagat
     3001 cccacaaact gaaaaaaata gcaacaaagt tttgggggaa gcattaaaat caagtttacc
     3061 cctctttacc tacataagag ccaacgtaat tttctctcag tattatatgt attatctccg
     3121 aagtttatta ttctttaacg aaatataaaa gaaaaataag aaagattaag accctaaaag
     3181 gtgtaccact acgattcaac aagcacaacg aatcctatcc gtaatatact tttgatttct
     3241 gactattcta gaaaaatttt agttcatttt ttttccctct ttttacttat tgtatttttc
     3301 acaattgaat tgcattttgt cccctacgaa aaaaggcaga aaacgagatc taaaccactt
     3361 tcagatcaga ataataataa taataataac caaccaaagc catcaacagg tccactagaa
     3421 gcatctgcca agttacgacc taagcaaagg tttgagtgaa aagtgaattg cgacagtaca
     3481 atcacaaaag tatagaaaca taattgttct cgagatggaa aacaaactac tttgttggtg
     3541 gttgtactgg ccatgcgtgt attcatctat tatagctacc ataatatctt tttacaccat
     3601 aactagacac ttattaaatt accgtaaacc atatgaacaa agactatcca tacggatact
     3661 attgctcgtg cccattttta gtgtgtcatg cgcaagtggc ataattaagc ctgaagcagc
     3721 tcaattttat gtcgatccta tcagagaatt ttatgaagca ttcgttattt acactttttt
     3781 cacattttta acactactac taggaggcga gaggaacatc attactgttt taagcttaaa
     3841 tcacgccccc acaagacatc cgataccgct aataggtaaa atttgcaaac cgatagactt
     3901 atctgatcct tttgattttc tttttgtcaa aaaaggcata ttacagtatg tttggttcaa
     3961 accattttat tgttttggta cattgatatg cagtgcatgg aagctaccca aattcgaaat
     4021 atttttgaac gttttttata atatttcagt gacttggtca ctgtatagtt tagcactatt
     4081 ttggaagtgt ctctatccgg aattaactcc ttataaacca tggttgaaat ttttatgtgt
     4141 caaattgatt atttttgcct cttattggca gagcatcata atccagggtc tagtggttac
     4201 tggaaagttg ggtacgggaa accaagatcg aacctctgga tatgtgtaca agaatgggct
     4261 cctgtgtata gagatggtgc ctttcgccat cttgcatgca gtagctttcc cttggaacaa
     4321 atacacagca tttagcatac cttatggggc caggatgaaa ttcatttacg ctttgaaaga
     4381 ttttcttggt tgcggtgatc taatatggga ttttaaacag actttgtttg ctggtccctt
     4441 gtactataat tataggaact tcgatcctga agccatggat ctattaagca ctagacaaca
     4501 atccggtgcg actatggaaa gactaaagca tgggctaaga tttactgata atggaagaaa
     4561 tagttattgg gtggcatatg ggagtataga taacaacctt gttccggaat caatagagga
     4621 atcatgggaa gatgatattg ctggtcagag aacttttcct gaggatccaa attatccggt
     4681 tgttcacgac tacacgatgg gccatcgata tagtagatca atgaatgatt taagaagaga
     4741 tgttcaaagt aggtcatcaa tggcttgtgg tcgtgggatt cctgggttaa ttaacatcgc
     4801 tagcgattat aaagatgatg atgataaacc tagcgattat aaagatgatg atgataaacc
     4861 tagcgattat aaagatgatg atgataaacc tagcgattat aaagatgatg atgataaacc
     4921 tagcgattat aaagatgatg atgataaacc tagcgattat aaagatgatg atgataaacc
     4981 tagcgattat aaagatgatg atgataaacc tagcgattat aaagatgatg atgataaacc
     5041 taggtgaggc gcgccacttc taaataagcg aatttcttat gatttatgat ttttattatt
     5101 aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta ggttttaaaa
     5161 cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc tcaggtatag
     5221 catgaggtcg ctcttattga ccacaccgag ctctaagcaa atagctaaat tatatacgaa
     5281 ttaatattat gattaagtgt ttacgtgagt gcgatatttt tattactatc ttatacagtt
     5341 gtatatactc tataaaatga gttgtctatt aattaacgcg atgaatgctt tctgggttta
     5401 cctctccaac aactctagtt tacttctcaa tacattcaat tgtatttgat ttgtcaatac
     5461 ttcatcatta atcaattcta tagttttgtt tttctcgttt atttccaaat ttaatgcatc
     5521 aattttatta ttcaatttgt cgttgatttt ggttaatgat tttatggttt gatctctggc
     5581 attgattgtt tgtgttagtt tttcattatt gataattaaa ttatttaagt tagttatcaa
     5641 ctcggtgttt tcaagtttca agttttcaat ttctttagag tttattagat ttgtcaaagt
     5701 ttctgaattg cttgattggt cctgtagaag agtatttgtt gttgtggata attgattcaa
     5761 tttttgagac aattgctgga aggcgttgaa atatctagca tcaatctcat ggtttttttc
     5821 ccgagagtct cgtagattca attgttttaa tatatcttgg gaccactctt gatttgaact
     5881 catggaaatt aaactgggtg ttgtgttgtg gtgtaatgat tgtaccccct ttgcttataa
     5941 ttgtgtggcc accgaagtcg gtgatgctgt cgccgaagaa gttaagaaaa tccttgctta
     6001 aaaagattct ctttttttat gatatttgta cataaacttt ataaatgaaa ttcataatag
     6061 aaacgacacg aaattacaaa atggaatatg ttcatagggt agacgaaact atatacgcaa
     6121 tctacataca tttatcaaga aggagaaaaa ggaggatgta aaggaataca ggtaagcaaa
     6181 ttgatactaa tggctcaacg tgataaggaa aaagaattgc actttaacat taatattgac
     6241 aaggaggagg gcaccacaca aaaagttagg tgtaacagaa aatcatgaaa ctatgattcc
     6301 taatttatat attggaggat tttctctaaa aaaaaaaaaa tacaacaaat aaaaaacact
     6361 caatgacctg accatttgat ggagtttaag tcaatacctc cttgaaccat ttcccataat
     6421 ggtgaaagtt ccctcaagaa ttttactctg tcagaaacgg ccttaacgac gtaggccggc
     6481 catttaaatg tttaaacggc cacgctggcc ccctatagtg agtcgtatta caattcactg
     6541 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     6601 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     6661 tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg
     6721 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     6781 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     6841 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     6901 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     6961 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     7021 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     7081 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     7141 ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     7201 agggtaataa ctgatataat taaattgaag ctctaatttg tgagtttagt attaggatct
     7261 ctacccttgg cgaaaagtcc tctgccaaca atgatgatat ctgatccacc acttacaact
     7321 tcgtcgacgg ttctgtactg ctgacccaat atgcgtatat ataccaatct aagtctgtgc
     7381 tccttccttc gttcttcctt ctgttcggag attaccgaat caaaaaaatt tcaaggaaac
     7441 cgaaatcaaa aaaaagaata aaaaaaaaat gatgaattga aaaggtggta tggtgcactc
     7501 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     7561 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     7621 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     7681 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     7741 cgtcggcgcg ccgaggacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     7801 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     7861 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     7921 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     7981 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     8041 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     8101 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     8161 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     8221 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     8281 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     8341 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     8401 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     8461 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     8521 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     8581 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     8641 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     8701 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     8761 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     8821 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     8881 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     8941 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     9001 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     9061 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     9121 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     9181 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     9241 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     9301 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     9361 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     9421 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     9481 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     9541 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     9601 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     9661 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     9721 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     9781 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     9841 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     9901 aaacagctat gaccatgatt acgccaagct cggaattaac cctcactaaa gggaacaaaa
     9961 gctg
//
