LOCUS       sequence-424904-        3242 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PrpsM driven expression of mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Delgadillo-Guevara M, Halte M, Erhardt M, Popp PF
  TITLE     Fluorescent tools for the standardized work in Gram-negative 
            bacteria.
  JOURNAL   J Biol Eng. 2024 Apr 8;18(1):25. doi: 10.1186/s13036-024-00420-9.
  PUBMED    38589953
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2024-04-8"; volume: "18(1):25. doi"; pages: " 
            10.1186/s13036-024-00420-9"
COMMENT     Sequence Label: pEM8733
FEATURES             Location/Qualifiers
     source          1..3242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1214..1300
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1337..1354)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1550..1569)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2053..2147)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2177..2968,2969..3037))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2800..2819
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3038..3142)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3210..3228
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgaggatc ccgggtacct gcagctagcg tcgacgaaag gctacggccg ttaattggtc
       61 gcctgagaag ttacggagag taaaaatgaa agttcgtgct tccgtcaaga aattatgccg
      121 taactgcaaa atcgttaagc gtgatggtgt catccgtgtg atttgcagtg ccgagccgaa
      181 gcataaacag cgccaaggct gattttttcg catatttttc ttgcaaagtt gggttgagct
      241 ggctagatta gccagccaat cttttgtatg tctgtacgtt tccatttgag tatcctgaaa
      301 acgggctttt cagcatggta cgtacatatt aaatagtagg agtgcatagt ggcccgtata
      361 gcaggcatta acattcctga tcagaaacac gccgtgatcg cgttaacttc gatctacggt
      421 gtcggcaaga cccgttctaa agccatcctg gctggaattc ataaggagga aaaacatatg
      481 gtttccaagg gcgaggagga taacatggct atcattaaag agttcatgcg cttcaaagtt
      541 cacatggagg gttctgttaa cggtcacgag ttcgagatcg aaggcgaagg cgagggccgt
      601 ccgtatgaag gcacccagac cgccaaactg aaagtgacta aaggcggccc gctgcctttt
      661 gcgtgggaca tcctgagccc gcaatttatg tacggttcta aagcgtatgt taaacaccca
      721 gcggatatcc cggactatct gaagctgtct tttccggaag gtttcaagtg ggaacgcgta
      781 atgaattttg aagatggtgg tgtcgtgacc gtcactcagg actcctccct gcaggatggc
      841 gagttcatct ataaagttaa actgcgtggt actaattttc catctgatgg cccggtgatg
      901 cagaaaaaga cgatgggttg ggaggcgtct agcgaacgca tgtatccgga agatggtgcg
      961 ctgaaaggcg aaattaaaca gcgcctgaaa ctgaaagatg gcggccatta tgacgctgaa
     1021 gtgaaaacca cgtacaaagc caagaaacct gtgcagctgc ctggcgcgta caatgtgaat
     1081 attaaactgg acatcacctc tcataatgaa gattatacga tcgtagagca atatgagcgc
     1141 gcggagggtc gtcattctac cggtggcatg gatgagctgt acaaataata ataagcggcc
     1201 gctctagagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     1261 ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgccct agacctaggc
     1321 ggagatattc cgcttcctcg ctcactgact cgctccgctc ggtcgttcgg ctgcggcgag
     1381 cggtaccggc ttactggcgg ggcggaaatt tcctggaaga tgccaggaag aaacttaaca
     1441 gggaagcgat aaggccacgg cagagccgtt tttccatagg ctccgccccc ctgacaagca
     1501 tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg acaggactta aagataccag
     1561 gcgtttcccc ctggtggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt
     1621 gtcactccgc tgttatggcc gcgtttatct cattccacgc ctgacactcg gttccgggaa
     1681 ggcagttcgc tccaagctgg actgtatgca cgaacccccc gttcagtccg actgctgcgc
     1741 ctgttccggt aactatcgac ttgagtccaa gccggaaaga cacgacaaag cgccactggc
     1801 agcagccact ggtaagaggg tgtacaagag attactgatg cagagttctt gaagtcagag
     1861 ccaaactacg gctaaactgg aaggacgaat ttggtggcag cggtcttgta caccctgtta
     1921 ccacggttca gaagttcccc aacttactga accttcgaaa aaccacctcc ccaggtggtt
     1981 ttttcgtttt cagagcaaga gattacgacg caacggaaag gatctcaaga ggttcatctt
     2041 actagtgctt ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa
     2101 atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcgt
     2161 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     2221 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     2281 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     2341 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     2401 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     2461 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     2521 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     2581 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     2641 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     2701 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     2761 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     2821 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     2881 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     2941 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     3001 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     3061 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     3121 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     3181 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc
     3241 ac
//
