LOCUS       sequence-424908-       10512 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAPT Exon 10 reporter containing the MS2 hairpin 30 base pairs 
            downstream of the 5′ splice site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10512)
  AUTHORS   Schmok JC, Jain M, Street LA, Tankka AT, Schafer D, Her HL, 
            Elmsaouri S, Gosztyla ML, Boyle EA, Jagannatha P, Luo EC, Kwon EJ, 
            Jovanovic M, Yeo GW
  TITLE     Large-scale evaluation of the ability of RNA-binding proteins to 
            activate exon inclusion.
  JOURNAL   Nat Biotechnol. 2024 Jan 2. doi: 10.1038/s41587-023-02014-0.
  PUBMED    38168984
REFERENCE   2  (bases 1 to 10512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Jan 2. doi: 10.1038/s41587-023-02014-0."
COMMENT     Sequence Label: lucMAPT-30D
FEATURES             Location/Qualifiers
     source          1..10512
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1040..1161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1051..1070)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1105..1124
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1335..2129)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1446..1465)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2056..2075
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2196..2525)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2210..2345
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2264..2283)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     2510..2530
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(2539..2967)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2649..2670)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2861..2880
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    3013..3237
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3226..3243
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(3287..4219)
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="ASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAENA
                     VIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAWF
                     ELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDIA
                     LIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPREI
                     PLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKVK
                     GLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     misc_RNA        complement(5271..5289)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     CDS             complement(6680..8329)
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /db_xref="GI:42718124"
                     /protein_id="AAS38485.1"
                     /translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFSAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     RBS             7362..7370
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(8336..8377)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     regulatory      8383..8392
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(8464..8483)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8465..8483)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(8528..8731)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8557..8577)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8732..9111
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     9283..9302
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        9377..9481
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9482..9550,9551..10342)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9700..9719)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
     1321 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     1381 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     1441 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
     1501 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     1561 tcgccatggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac
     1621 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     1681 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     1741 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     1801 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     1861 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     1921 agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc
     1981 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     2041 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     2101 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca
     2161 gatccgaaaa tggatataca agctcccggg agctttttgc aaaagcctag gcctccaaaa
     2221 aagcctcctc actacttctg gaatagctca gaggcagagg cggcctcggc ctctgcataa
     2281 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc
     2341 gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc
     2401 atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga
     2461 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga
     2521 cacacattcc acagaattaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     2581 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     2641 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     2701 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     2761 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     2821 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     2881 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     2941 cgcttaatgc gccgctacag ggcgcgtggg gataccccct agagccccag ctggttcttt
     3001 ccgcctcaga agccatagag cccaccgcat ccccagcatg cctgctattg tcttcccaat
     3061 cctccccctt gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac
     3121 aatgcgatgc aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg
     3181 tcaaggaagg cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg
     3241 aggctgatca gcggtttaaa cttaagcttg gtaccgagct cggatctcac tgctcgttct
     3301 tcagcacgcg ctccacgaag ctcttgatgt acttacccat ttcatctgga gcgtcctcct
     3361 ggctgaagtg gaggcccttc accttcacga actcggtgtt agggaacttc ttagctccct
     3421 cgacaatagc gttggaaaag aacccagggt cggactcgat gaacatctta ggcagatcgt
     3481 cgctggcccg aaggtaggcg ttgtagttgc ggacaatctg gacgacgtcg ggcttgcctc
     3541 ccttaacgag agggatctcg cgaggccagg agagggtagg ccgtctaacc tcgcccttct
     3601 ccttgaatgg ctccaggtag gcagcgaact cctcaggctc cagtttccgc atgatcttgc
     3661 ttgggagcat ggtctcgacg aagaagttat tctcaagcac cattttctcg ccctcttcgc
     3721 tcttgatcag ggcgatatcc tcctcgatgt caggccactc gtcccaggac tcgatcacgt
     3781 ccacgacact ctcagcatgg acgatggcct tgatcttgtc ttggtgctcg taggagtagt
     3841 gaaaggccag acaagccccc cagtcgtggc ccacaaagat gattttcttt ggaaggttca
     3901 gcagctcgaa ccaagcggtg aggtacttgt agtgatccag gaggcgatat gagccattcc
     3961 cgctcttgcc ggacttaccc attccgatca gatcagggat gatgcatcta gccacgggct
     4021 cgatgtgagg cacgacgtgc ctccacaggt agctggaggc agcgttacca tgcagaaaaa
     4081 tcacggcgtt ctcggcgtgc ttctcggaat catagtagtt gatgaaggag tccagcacgt
     4141 tcatttgctt gcagcgagcc caccactgag gcccagtgat catgcgtttg cgttgctcgg
     4201 ggtcatacac cttggaagct ggtttatgat ggatgttgcc taatgagcca cacttggagg
     4261 tcaccttgct caggtcaact ggtttgtaga ctatttgcac ctggagatga gagaggagga
     4321 gagagaagag gaaaggcaga gagagaggaa gacagagaga caaaaaggat aaaatgggat
     4381 tattttatta agggtgtaac tcaatgagaa gcagctgtgt aagcccaggc ccttattgga
     4441 cagtggttcc cagctgccat gaggagcaca tcctagtcag ggtggaaaaa tgtgttgtcg
     4501 aaattctgcc aaccctgccc caggggagct ggggactgtg ggactggagt gtcttcaatc
     4561 cctgcagaac aaaagagcct ctgggaaaaa cagggaccca ttgtcattgt gcaagaccca
     4621 gacatttgct cagcaaacgc ttaagtgtaa aaatgtcact ccctttcctg acatgaaatt
     4681 gacagatcac aatcaggcat gaagaaaaaa gtgatcaata tgatgagtat gcacagcatc
     4741 tgaaaaatct tgctggaaag gcaggagtgg ggtatctgct gggcctcaaa gaggggaggc
     4801 gtccgctcat cagaacaaac gggtcacctg tccacagtgg agccactcaa cgtcaacctg
     4861 cccaggtgtc gggggaggag gcggcagccc agtctcaggt gccaccacct tcagcccaac
     4921 ttccaatggg ctatggggct cctggggaca gggctgggca tgagtggaag gagactttga
     4981 tgggtattct cagcttccca ggcagggtcc ggagagaggg tccgtcatct gccctattct
     5041 gtccacacag cctgcgcagg ctgggggacc cagaaatgct gggacacccc ctcctagaat
     5101 atgaggaagg ggcttctggg aacagtggac cgtgtggggg aatgtgggga gccgggctac
     5161 attcacccag aggtcgcagc cagatcctga gagcccaaga aggatttatt ctatgcagtg
     5221 tctcgcaagt gtacgcactc acaccacttc ctaataattc aagccacagc acatgggtga
     5281 tcctcatgta cggcgcatgg gacgtgtgaa ggtactcaca ctgccgcctc ccgggacgtg
     5341 ttagatatta tcctttgagc cacacttgga ctggacgttg ctaagatcca gcttcttatt
     5401 aattatctgc acctttggta gccagaaaaa aggatgagtg acacgccacc ctggacccgc
     5461 ctgcttgctc gcaaggacgc ctccactttc gatgagtgac atgcaccacc ctggacccgc
     5521 ctacttgctc gcaaggatgc ctccactttc ggacttggca gaggtagtct ggggagtgac
     5581 gggaggttga gtcacatccc aggggagctg aagggataga gggctgctct gaaggcatcc
     5641 cagaagtgtg agtgatttcc agcgggcttt acgaagctgg gattgcaggt cctcacttca
     5701 caaaaggatg gcatcgtgag gcctgaaccc cagaggggag cgttcaccag cctgaggtca
     5761 agtgaaaaat gtgttcggcc acactgacga gttagtaata ggaagttccc cggcatctcc
     5821 actcacacct ggctagaccc accggcacat tcccagagaa ctccctgcgg ctgttctcaa
     5881 gcctgccggg catcagaatc acctgtggat ttctcccaga agaatcagct gatcaaaaca
     5941 gaacccccaa tctccccaga atccagagga acccaaccta agcagatggc ccacgagtgg
     6001 agatgctgac ctatgggtgg cagtgtattc tgcccagaag gcaggtgggg aaccactgtg
     6061 ggtgccctga ctatgagagc ctccggccgt ggagacacag ccctgccatc tctgtccgat
     6121 taacaggggc tgaaggggca agagaagagc cagaacgttc tctcgcctga ggagggcact
     6181 cacagtgtag tggagagccc aataaagaac tgaacttcct tgaagagggt ccagagggga
     6241 ctggggtgtt atgcgggggt gctcaggtgc ggggagcccc acacctccat gcacagtccc
     6301 acgactccac gctcaaccgc gcacctccag gcgcagccct acccttccag gcacagccct
     6361 acccctccag gcgcagcccc ccacacctcc acgcgcagcc agccactctc accttcccgc
     6421 ctcccggctg gtgcttcagg ttctcagtgg agccgatctt ggacttgaca ttcttcaggt
     6481 ctggcatggg cacgggggct gtctgcaggc ggctcttggc ggaagacggc gacttgggtg
     6541 gagtacggac cactgccacc ttcttgggct cccgggtggg tggggttgga agggacgggg
     6601 tgcgggagcg gctgctggga gtgcctgggg agccggggct gctgtagccg ctgcgatccc
     6661 ctgattttgg aggttcactc acggcgatct tgccgccttt cttagccttg atcaggatct
     6721 cgcggatctt gcgggcgtcc agcttgccgg tcaggccctt gggcacctcg tccacgaaca
     6781 ccactccgcc gcgcagcttc ttggcggttg tcacctggct ggccacatag tccacgatct
     6841 ccttctcggt catggtcttg ccgtgttcca gcacgacgac ggcggcaggc agctcgccgg
     6901 catcgtcgtc gggcaggccg gccactccgg cgtcgaaaat gttagggtgc tgcagcagga
     6961 tagactccag ctcggctggg gccacctggt agcccttgta cttgatcaga gacttcaggc
     7021 ggtccacgat gaagaagtgc tcgtcctcgt cccagtaggc aatgtcgcca gagtgcagcc
     7081 agccgtcctt gtcgatcagg gcgtttgtgg cctcagggtt attcacgtag ccggacataa
     7141 tcatagggcc gcgcacacac agctcgccgc gctggttcac tcccagggtc ttgccggtgt
     7201 ccaggtccac caccttggcc tcgaagaatg gcaccacctt gcccacggcg ccaggcttgt
     7261 cgtcgccctc tggggtaatc agaatggcgc tggttgtctc ggtcaggccg tagccctggc
     7321 ggatgcctgg cagatgaaag cgcttggcca cggcttcgcc cacctcctta gacagtgggg
     7381 cgccgccaga ggcaatctcg tgcaggttag acaggtcgta cttgtcgatc agggtgctct
     7441 tagcggagaa gctgaacagg gttggcacca gcagggcaga ctgaattttg tagtcttgca
     7501 ggctgcgcag gaacagctcc tcctcgaagc ggtacatcag caccacccga aagccgcaaa
     7561 tcaggtagcc cagggtggtg aacatgccga agccgtggtg gaatggcacc acgctcagaa
     7621 tagcggtgtc ggggatgatc tggttgccga aaatagggtc gcgggcgtga gagaagcgca
     7681 cacaggcggt gcgatgaggc agggccacgc ccttaggcag gccggtagac ccagagctgt
     7741 tcatgatcag ggcaatggtt ttgtccctgt cgaaagactc tggcacgaag tcgtactcgt
     7801 tgaagccagg aggcagatga gatgtcacga atgtgtacat gctctggaag ccctggtagt
     7861 cggtcttaga gtccatgatg atgatcttct ggatgatagg cagcttcttc tgcacgttca
     7921 ggatcttctg caggcccttc ttagacacga acaccacggt aggctgagaa atgcccatgc
     7981 tgttcagcag ctcgcgctcg ttgtaaatgt cgttagcagg ggccacggcc actccgatga
     8041 acagggcgcc cagcactggc atgaagaact gcagagagtt ctcagagcac accacgatgc
     8101 ggtggttggt gttcaggccg tacctcttca tggcctcggc caggcgcaca gacatctcga
     8161 agtactcggc ataggtgatg tccacctcaa tgtgggcatc ggtgaaggca atggtgccag
     8221 gcaccagggc atacctcttc atggccttgt gcagctgctc gccagcggtg ccatcctcca
     8281 gagggtagaa gggagcaggg cccttcttaa tgttcttagc atcggccatg ctgcccgtag
     8341 aatcgagacc gaggagaggg ttagggatag gcttaccgct gcccatggtg gctgcagata
     8401 tccagcacag tggcggccgc tcgagtctag agggcccgtt taaacgctag ccagcttggg
     8461 tctccctata gtgagtcgta ttaatttcga taagccagta agcagtgggt tctctagtta
     8521 gccagagagc tctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca
     8581 atggggcgga gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac
     8641 tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac
     8701 gcccattgat gtactgccaa aaccgcatca ccatggtaat agcgatgact aatacgtaga
     8761 tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc
     8821 atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg
     8881 ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg
     8941 gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc
     9001 agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat gggctatgaa
     9061 ctaatgaccc cgtaattgat tactattaat aactagtcaa taatcaatgt caacgcgtat
     9121 atctggcccg tacatcgcga agcagcgcaa aacgcctaac cctaagcaga ttcttcatgc
     9181 aattgtcggt caagccttgc cttgttgtag cttaaatttt gctcgcgcac tactcagcga
     9241 cctccaacac acaagcaggg agcagatact ggcttaacta tgcggcatca gagcagattg
     9301 tactgagagt gcaccatagg ggatcgggag atctcccgat ccgtcgacgt caggtggcac
     9361 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     9421 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     9481 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     9541 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     9601 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     9661 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     9721 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     9781 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     9841 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     9901 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     9961 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
    10021 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
    10081 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
    10141 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
    10201 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
    10261 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
    10321 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
    10381 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
    10441 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
    10501 caaaggatct tc
//
