LOCUS       sequence-424909-        2849 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PrpsM driven expression of miniSOG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Delgadillo-Guevara M, Halte M, Erhardt M, Popp PF
  TITLE     Fluorescent tools for the standardized work in Gram-negative 
            bacteria.
  JOURNAL   J Biol Eng. 2024 Apr 8;18(1):25. doi: 10.1186/s13036-024-00420-9.
  PUBMED    38589953
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2024-04-8"; volume: "18(1):25. doi"; pages: " 
            10.1186/s13036-024-00420-9"
COMMENT     Sequence Label: pEM10689
FEATURES             Location/Qualifiers
     source          1..2849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             478..798
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     terminator      821..907
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(944..961)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1157..1176)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1660..1754)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1784..2575,2576..2644))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2407..2426
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2645..2749)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2817..2835
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgaggatc ccgggtacct gcagctagcg tcgacgaaag gctacggccg ttaattggtc
       61 gcctgagaag ttacggagag taaaaatgaa agttcgtgct tccgtcaaga aattatgccg
      121 taactgcaaa atcgttaagc gtgatggtgt catccgtgtg atttgcagtg ccgagccgaa
      181 gcataaacag cgccaaggct gattttttcg catatttttc ttgcaaagtt gggttgagct
      241 ggctagatta gccagccaat cttttgtatg tctgtacgtt tccatttgag tatcctgaaa
      301 acgggctttt cagcatggta cgtacatatt aaatagtagg agtgcatagt ggcccgtata
      361 gcaggcatta acattcctga tcagaaacac gccgtgatcg cgttaacttc gatctacggt
      421 gtcggcaaga cccgttctaa agccatcctg gctggaattc ataaggagga aaaacatatg
      481 gaaaaaagct ttgtgattac cgatccgcgc ctgccggata acccgattat ttttgcgagc
      541 gatggctttc tggaactgac cgaatatagc cgcgaagaaa ttctgggccg caacggccgc
      601 tttctgcagg gcccggaaac cgatcaggcg accgtgcaga aaattcgcga tgcgattcgc
      661 gatcagcgcg aaattaccgt gcagctgatt aactatacca aaagcggcaa aaaattttgg
      721 aacctgctgc atctgcagcc gatgcgcgat cagaaaggcg aactgcagta ttttattggc
      781 gtgcagctgg atggctaatg agcggccgct ctagaggcat caaataaaac gaaaggctca
      841 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag
      901 gacaaatccg ccgccctaga cctaggcgga gatattccgc ttcctcgctc actgactcgc
      961 tccgctcggt cgttcggctg cggcgagcgg taccggctta ctggcggggc ggaaatttcc
     1021 tggaagatgc caggaagaaa cttaacaggg aagcgataag gccacggcag agccgttttt
     1081 ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggtggcg
     1141 aaacccgaca ggacttaaag ataccaggcg tttccccctg gtggctccct cgtgcgctct
     1201 cctgttcctg cctttcggtt taccggtgtc actccgctgt tatggccgcg tttatctcat
     1261 tccacgcctg acactcggtt ccgggaaggc agttcgctcc aagctggact gtatgcacga
     1321 accccccgtt cagtccgact gctgcgcctg ttccggtaac tatcgacttg agtccaagcc
     1381 ggaaagacac gacaaagcgc cactggcagc agccactggt aagagggtgt acaagagatt
     1441 actgatgcag agttcttgaa gtcagagcca aactacggct aaactggaag gacgaatttg
     1501 gtggcagcgg tcttgtacac cctgttacca cggttcagaa gttccccaac ttactgaacc
     1561 ttcgaaaaac cacctcccca ggtggttttt tcgttttcag agcaagagat tacgacgcaa
     1621 cggaaaggat ctcaagaggt tcatcttact agtgcttgga ttctcaccaa taaaaaacgc
     1681 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc
     1741 tatcaacagg agtccaagcg agctcgtaaa cttggtctga cagttaccaa tgcttaatca
     1801 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     1861 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     1921 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     1981 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     2041 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     2101 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     2161 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     2221 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     2281 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     2341 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     2401 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     2461 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     2521 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     2581 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     2641 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     2701 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     2761 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     2821 ggcgtatcac gaggcccttt cgtcttcac
//
