LOCUS       sequence-424920-        3315 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PrpsM driven expression of SSAGSAAGS-mGFPmut2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Delgadillo-Guevara M, Halte M, Erhardt M, Popp PF
  TITLE     Fluorescent tools for the standardized work in Gram-negative 
            bacteria.
  JOURNAL   J Biol Eng. 2024 Apr 8;18(1):25. doi: 10.1186/s13036-024-00420-9.
  PUBMED    38589953
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2024-04-8"; volume: "18(1):25. doi"; pages: " 
            10.1186/s13036-024-00420-9"
COMMENT     Sequence Label: pEM9177
FEATURES             Location/Qualifiers
     source          1..3315
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1287..1373
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1410..1427)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1623..1642)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2126..2220)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2250..3041,3042..3110))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2873..2892
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3111..3215)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3283..3301
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgaggatc ccagatggag ttctgaggtc acgaaaggct acggccgtta attggtcgcc
       61 tgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
      121 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
      181 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
      241 tagattagcc agccaatctt ttgtatgtct gtacgtttcc atttgagtat cctgaaaacg
      301 ggcttttcag catggtacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
      361 ggcattaaca ttcctgatca gaaacacgcc gtgatcgcgt taacttcgat ctacggtgtc
      421 ggcaagaccc gttctaaagc catcctggct ggatcggctt attccctaac taattaaaga
      481 ttaaccccgg gaggaaaaac atatgagctc ggctggctcc gctgctggtt ctggcgaatt
      541 cgtggtcagt aagggggagg aattgttcac gggtgttgtt ccgattttgg tcgaacttga
      601 cggagacgtg aatggtcaca aattctctgt ctccggcgag ggggagggtg atgctaccta
      661 tgggaaattg acgttgaaat tcatttgcac cacgggaaag ttgcctgtac cgtggcccac
      721 tcttgtcaca acgtttgcct atggattgca atgcttcgcc cggtatcctg accacatgaa
      781 acagcacgac ttctttaaat cggcgatgcc cgaaggctat gtccaggaac gtaccatttt
      841 ttttaaggac gatggaaatt acaaaacgcg ggcagaagtg aaatttgagg gggatacgct
      901 tgtaaaccgt atcgaactga agggaattga tttcaaggag gatggcaaca ttttggggca
      961 caagttagag tacaattaca actctcacaa cgtttatatc atggctgata aacaaaagaa
     1021 tggaatcaag gtgaatttta aaatccgcca caatattgag gacggaagtg tgcaactggc
     1081 tgatcactat caacagaata cccctatcgg cgatggcccc gtattgctgc ccgacaatca
     1141 ctatttaagt acacaaagta agctttcgaa agaccccaat gaaaagcggg accacatggt
     1201 ccttctggag tttgtaactg ccgctggcat cacacacggc atggatgagt tatataaatg
     1261 ataataagcg gccgctctag aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     1321 cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgc
     1381 cctagaccta ggcggagata ttccgcttcc tcgctcactg actcgctccg ctcggtcgtt
     1441 cggctgcggc gagcggtacc ggcttactgg cggggcggaa atttcctgga agatgccagg
     1501 aagaaactta acagggaagc gataaggcca cggcagagcc gtttttccat aggctccgcc
     1561 cccctgacaa gcatcacgaa atctgacgct caaatcagtg gtggcgaaac ccgacaggac
     1621 ttaaagatac caggcgtttc cccctggtgg ctccctcgtg cgctctcctg ttcctgcctt
     1681 tcggtttacc ggtgtcactc cgctgttatg gccgcgttta tctcattcca cgcctgacac
     1741 tcggttccgg gaaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt
     1801 ccgactgctg cgcctgttcc ggtaactatc gacttgagtc caagccggaa agacacgaca
     1861 aagcgccact ggcagcagcc actggtaaga gggtgtacaa gagattactg atgcagagtt
     1921 cttgaagtca gagccaaact acggctaaac tggaaggacg aatttggtgg cagcggtctt
     1981 gtacaccctg ttaccacggt tcagaagttc cccaacttac tgaaccttcg aaaaaccacc
     2041 tccccaggtg gttttttcgt tttcagagca agagattacg acgcaacgga aaggatctca
     2101 agaggttcat cttactagtg cttggattct caccaataaa aaacgcccgg cggcaaccga
     2161 gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc
     2221 caagcgagct cgtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     2281 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     2341 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     2401 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     2461 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     2521 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     2581 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     2641 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     2701 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     2761 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     2821 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     2881 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     2941 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     3001 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     3061 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     3121 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     3181 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     3241 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     3301 ccctttcgtc ttcac
//
