LOCUS       sequence-424923-        3248 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PrpsM driven expression of mCerulean.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Delgadillo-Guevara M, Halte M, Erhardt M, Popp PF
  TITLE     Fluorescent tools for the standardized work in Gram-negative 
            bacteria.
  JOURNAL   J Biol Eng. 2024 Apr 8;18(1):25. doi: 10.1186/s13036-024-00420-9.
  PUBMED    38589953
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2024-04-8"; volume: "18(1):25. doi"; pages: " 
            10.1186/s13036-024-00420-9"
COMMENT     Sequence Label: pEM8313
FEATURES             Location/Qualifiers
     source          1..3248
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1220..1306
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1343..1360)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1556..1575)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2059..2153)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2183..2974,2975..3043))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2806..2825
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3044..3148)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3216..3234
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgaggatc ccgggtacct gcagctagcg tcgacgaaag gctacggccg ttaattggtc
       61 gcctgagaag ttacggagag taaaaatgaa agttcgtgct tccgtcaaga aattatgccg
      121 taactgcaaa atcgttaagc gtgatggtgt catccgtgtg atttgcagtg ccgagccgaa
      181 gcataaacag cgccaaggct gattttttcg catatttttc ttgcaaagtt gggttgagct
      241 ggctagatta gccagccaat cttttgtatg tctgtacgtt tccatttgag tatcctgaaa
      301 acgggctttt cagcatggta cgtacatatt aaatagtagg agtgcatagt ggcccgtata
      361 gcaggcatta acattcctga tcagaaacac gccgtgatcg cgttaacttc gatctacggt
      421 gtcggcaaga cccgttctaa agccatcctg gctggaattc ataaggagga aaaacatatg
      481 gttagtaaag gggaggagtt atttactggt gtagttccga ttttagtcga gcttgacggg
      541 gatgtaaatg ggcacaaatt tagtgtctct ggtgaaggcg agggtgatgc tacttacggg
      601 aaacttacgc tgaagtttat ctgtaccact ggaaagcttc cggttccctg gcccaccttg
      661 gttacaacat tgacatgggg tgtccaatgc ttcgcgcgct atcccgatca tatgaaacag
      721 cacgatttct tcaagtctgc catgccggag ggttacgttc aagaacgcac aatctttttc
      781 aaggatgatg gaaattacaa aactcgtgcg gaagtaaagt tcgagggtga tacccttgtg
      841 aaccgtattg agttgaaggg gattgatttt aaagaggacg gcaacatttt agggcacaaa
      901 ttagagtata atgctatctc ggacaacgta tatatcacag cggacaaaca gaaaaacggc
      961 atcaaagcta acttcaaaat tcgtcataat attgaggatg ggtctgttca actggcggat
     1021 cattaccagc agaacactcc aattggggac ggcccagtct tgttaccgga taaccattat
     1081 ttgagcacgc agtctaaatt gtcgaaagat cccaatgaaa aacgggatca catggtactg
     1141 ttggagttcg tcacggcggc cgggattact ttggggatgg acgagttata taagtgataa
     1201 gcggccgctc tagaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1261 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgccctagac
     1321 ctaggcggag atattccgct tcctcgctca ctgactcgct ccgctcggtc gttcggctgc
     1381 ggcgagcggt accggcttac tggcggggcg gaaatttcct ggaagatgcc aggaagaaac
     1441 ttaacaggga agcgataagg ccacggcaga gccgtttttc cataggctcc gcccccctga
     1501 caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag gacttaaaga
     1561 taccaggcgt ttccccctgg tggctccctc gtgcgctctc ctgttcctgc ctttcggttt
     1621 accggtgtca ctccgctgtt atggccgcgt ttatctcatt ccacgcctga cactcggttc
     1681 cgggaaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgactg
     1741 ctgcgcctgt tccggtaact atcgacttga gtccaagccg gaaagacacg acaaagcgcc
     1801 actggcagca gccactggta agagggtgta caagagatta ctgatgcaga gttcttgaag
     1861 tcagagccaa actacggcta aactggaagg acgaatttgg tggcagcggt cttgtacacc
     1921 ctgttaccac ggttcagaag ttccccaact tactgaacct tcgaaaaacc acctccccag
     1981 gtggtttttt cgttttcaga gcaagagatt acgacgcaac ggaaaggatc tcaagaggtt
     2041 catcttacta gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct
     2101 gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga
     2161 gctcgtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     2221 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     2281 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     2341 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     2401 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     2461 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     2521 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     2581 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     2641 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     2701 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     2761 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     2821 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     2881 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     2941 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     3001 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     3061 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     3121 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     3181 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     3241 gtcttcac
//
