LOCUS       sequence-424924-        3263 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PrpsM driven expression of mNeptune2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Delgadillo-Guevara M, Halte M, Erhardt M, Popp PF
  TITLE     Fluorescent tools for the standardized work in Gram-negative 
            bacteria.
  JOURNAL   J Biol Eng. 2024 Apr 8;18(1):25. doi: 10.1186/s13036-024-00420-9.
  PUBMED    38589953
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2024-04-8"; volume: "18(1):25. doi"; pages: " 
            10.1186/s13036-024-00420-9"
COMMENT     Sequence Label: pEM10687
FEATURES             Location/Qualifiers
     source          1..3263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1235..1321
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1358..1375)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1571..1590)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2074..2168)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2198..2989,2990..3058))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2821..2840
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3059..3163)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3231..3249
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgaggatc ccgggtacct gcagctagcg tcgacgaaag gctacggccg ttaattggtc
       61 gcctgagaag ttacggagag taaaaatgaa agttcgtgct tccgtcaaga aattatgccg
      121 taactgcaaa atcgttaagc gtgatggtgt catccgtgtg atttgcagtg ccgagccgaa
      181 gcataaacag cgccaaggct gattttttcg catatttttc ttgcaaagtt gggttgagct
      241 ggctagatta gccagccaat cttttgtatg tctgtacgtt tccatttgag tatcctgaaa
      301 acgggctttt cagcatggta cgtacatatt aaatagtagg agtgcatagt ggcccgtata
      361 gcaggcatta acattcctga tcagaaacac gccgtgatcg cgttaacttc gatctacggt
      421 gtcggcaaga cccgttctaa agccatcctg gctggaattc ataaggagga aaaacatatg
      481 gtgagcaaag gcgaagaact gattaaagaa aacatgcata tgaaactgta tatggaaggc
      541 accgtgaaca accatcattt taaatgcacc agcgaaggcg aaggcaaacc gtatgaaggc
      601 acccagaccg gccgcattaa agtggtggaa ggcggcccgc tgccgtttgc gtttgatatt
      661 ctggcgacct gctttatgta tggcagcaaa acctttatta accataccca gggcattccg
      721 gattttttta aacagagctt tccggaaggc tttacctggg aacgcgtgac cacctatgaa
      781 gatggcggcg tgctgaccgt gacccaggat accagcctgc aggatggctg cctgatttat
      841 aacgtgaaac tgcgcggcgt gaactttccg agcaacggcc cggtgatgca gaaaaaaacc
      901 ctgggctggg aagcgagcac cgaaaccctg tatccggcgg atggcggcct ggaaggccgc
      961 tgcgatatgg cgctgaaact ggtgggcggc ggccatctgc attgcaacct gaaaaccacc
     1021 tatcgcagca aaaaaccggc gaaaaacctg aaaatgccgg gcgtgtattt tgtggatcgc
     1081 cgcctggaac gcattaaaga agcggataac gaaacctatg tggaacagca tgaagtggcg
     1141 gtggcgcgct attgcgatct gccgagcaaa ctgggccata aactgaacgg catggatgaa
     1201 ctgtataaat aatgagcggc cgctctagag gcatcaaata aaacgaaagg ctcagtcgaa
     1261 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
     1321 tccgccgccc tagacctagg cggagatatt ccgcttcctc gctcactgac tcgctccgct
     1381 cggtcgttcg gctgcggcga gcggtaccgg cttactggcg gggcggaaat ttcctggaag
     1441 atgccaggaa gaaacttaac agggaagcga taaggccacg gcagagccgt ttttccatag
     1501 gctccgcccc cctgacaagc atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc
     1561 gacaggactt aaagatacca ggcgtttccc cctggtggct ccctcgtgcg ctctcctgtt
     1621 cctgcctttc ggtttaccgg tgtcactccg ctgttatggc cgcgtttatc tcattccacg
     1681 cctgacactc ggttccggga aggcagttcg ctccaagctg gactgtatgc acgaaccccc
     1741 cgttcagtcc gactgctgcg cctgttccgg taactatcga cttgagtcca agccggaaag
     1801 acacgacaaa gcgccactgg cagcagccac tggtaagagg gtgtacaaga gattactgat
     1861 gcagagttct tgaagtcaga gccaaactac ggctaaactg gaaggacgaa tttggtggca
     1921 gcggtcttgt acaccctgtt accacggttc agaagttccc caacttactg aaccttcgaa
     1981 aaaccacctc cccaggtggt tttttcgttt tcagagcaag agattacgac gcaacggaaa
     2041 ggatctcaag aggttcatct tactagtgct tggattctca ccaataaaaa acgcccggcg
     2101 gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa
     2161 caggagtcca agcgagctcg taaacttggt ctgacagtta ccaatgctta atcagtgagg
     2221 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     2281 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     2341 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     2401 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     2461 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     2521 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     2581 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     2641 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     2701 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     2761 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     2821 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     2881 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     2941 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     3001 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     3061 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     3121 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     3181 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     3241 tcacgaggcc ctttcgtctt cac
//
