LOCUS       sequence-424925-        3219 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PrpsM driven expression of mScarlet-I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Delgadillo-Guevara M, Halte M, Erhardt M, Popp PF
  TITLE     Fluorescent tools for the standardized work in Gram-negative 
            bacteria.
  JOURNAL   J Biol Eng. 2024 Apr 8;18(1):25. doi: 10.1186/s13036-024-00420-9.
  PUBMED    38589953
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2024-04-8"; volume: "18(1):25. doi"; pages: " 
            10.1186/s13036-024-00420-9"
COMMENT     Sequence Label: pEM10686
FEATURES             Location/Qualifiers
     source          1..3219
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1191..1277
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1314..1331)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1527..1546)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2030..2124)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2154..2945,2946..3014))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2777..2796
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3015..3119)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3187..3205
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgaggatc ccgggtacct gcagctagcg tcgacgaaag gctacggccg ttaattggtc
       61 gcctgagaag ttacggagag taaaaatgaa agttcgtgct tccgtcaaga aattatgccg
      121 taactgcaaa atcgttaagc gtgatggtgt catccgtgtg atttgcagtg ccgagccgaa
      181 gcataaacag cgccaaggct gattttttcg catatttttc ttgcaaagtt gggttgagct
      241 ggctagatta gccagccaat cttttgtatg tctgtacgtt tccatttgag tatcctgaaa
      301 acgggctttt cagcatggta cgtacatatt aaatagtagg agtgcatagt ggcccgtata
      361 gcaggcatta acattcctga tcagaaacac gccgtgatcg cgttaacttc gatctacggt
      421 gtcggcaaga cccgttctaa agccatcctg gctggaattc ataaggagga aaaacatatg
      481 gtgagcaaag gcgaagcggt gattaaagaa tttatgcgct ttaaagtgca tatggaaggc
      541 agcatgaacg gccatgaatt tgaaattgaa ggcgaaggcg aaggccgccc gtatgaaggc
      601 acccagaccg cgaaactgaa agtgaccaaa ggcggcccgc tgccgtttag ctgggatatt
      661 ctgagcccgc agtttatgta tggcagccgc gcgtttatta aacatccggc ggatattccg
      721 gattattata aacagagctt tccggaaggc tttaaatggg aacgcgtgat gaactttgaa
      781 gatggcggcg cggtgaccgt gacccaggat accagcctgg aagatggcac cctgatttat
      841 aaagtgaaac tgcgcggcac caactttccg ccggatggcc cggtgatgca gaaaaaaacc
      901 atgggctggg aagcgagcac cgaacgcctg tatccggaag atggcgtgct gaaaggcgat
      961 attaaaatgg cgctgcgcct gaaagatggc ggccgctatc tggcggattt taaaaccacc
     1021 tataaagcga aaaaaccggt gcagatgccg ggcgcgtata acgtggatcg caaactggat
     1081 attaccagcc ataacgaaga ttataccgtg gtggaacagt atgaacgcag cgaaggccgc
     1141 catagcaccg gcggcatgga tgaactgtat aaataataat ctagaggcat caaataaaac
     1201 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     1261 tcctgagtag gacaaatccg ccgccctaga cctaggcgga gatattccgc ttcctcgctc
     1321 actgactcgc tccgctcggt cgttcggctg cggcgagcgg taccggctta ctggcggggc
     1381 ggaaatttcc tggaagatgc caggaagaaa cttaacaggg aagcgataag gccacggcag
     1441 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
     1501 agtggtggcg aaacccgaca ggacttaaag ataccaggcg tttccccctg gtggctccct
     1561 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc actccgctgt tatggccgcg
     1621 tttatctcat tccacgcctg acactcggtt ccgggaaggc agttcgctcc aagctggact
     1681 gtatgcacga accccccgtt cagtccgact gctgcgcctg ttccggtaac tatcgacttg
     1741 agtccaagcc ggaaagacac gacaaagcgc cactggcagc agccactggt aagagggtgt
     1801 acaagagatt actgatgcag agttcttgaa gtcagagcca aactacggct aaactggaag
     1861 gacgaatttg gtggcagcgg tcttgtacac cctgttacca cggttcagaa gttccccaac
     1921 ttactgaacc ttcgaaaaac cacctcccca ggtggttttt tcgttttcag agcaagagat
     1981 tacgacgcaa cggaaaggat ctcaagaggt tcatcttact agtgcttgga ttctcaccaa
     2041 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
     2101 ttactggatc tatcaacagg agtccaagcg agctcgtaaa cttggtctga cagttaccaa
     2161 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     2221 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     2281 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     2341 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     2401 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     2461 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     2521 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     2581 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     2641 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     2701 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     2761 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     2821 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     2881 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     2941 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     3001 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     3061 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     3121 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     3181 tataaaaata ggcgtatcac gaggcccttt cgtcttcac
//
