LOCUS       sequence-424926-        8015 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8015)
  TITLE     Patrick Moore plasmids
REFERENCE   2  (bases 1 to 8015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA6.MCV.LT206.V5(CM2B4)
FEATURES             Location/Qualifiers
     source          1..8015
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..65)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        237..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        617..820
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     771..791
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     865..884
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        865..883
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3882..3923
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3933..3950
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3973..3990)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3979..4203
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4249..4677
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4336..4355)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4546..4567
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4686..4706)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4691..5020
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4871..5006
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4933..4952
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5068..5115
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5134..5532
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    5690..5811
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5727..5746)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5781..5800
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5860..5876)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5860..5876)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5873..5895)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5884..5900
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5908..5914,5915..5932,5933..5938))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5953..5974
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6091..6108)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6262..6850)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6342..6361)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7021..7812,7813..7881))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7644..7663
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7882..7986)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc
      841 cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc aagctggcta
      901 gcgtttaaac ttaagcttgg taccgagctc ggatccacta gtccagtgtg gtggaattct
      961 gcagatatca tggatttagt cctaaatagg aaagaaagag aggctctctg caagctttta
     1021 gagattgctc ctaattgtta tggcaacatc cctctgatga aagctgcttt caaaagaagc
     1081 tgcttaaagc atcaccctga taaaggggga aatcctgtta taatgatgga attgaacacc
     1141 ctttggagca aattccagca aaatatccac aagctcagaa gtgacttctc tatgtttgat
     1201 gaggtcagta caaaatttcc ttgggaagaa tatggaactt taaaggatta tatgcaaagt
     1261 ggatataatg ctagattttg cagaggtcct gggtgcatgc ttaagcaact tagagattct
     1321 aagtgcgctt gtattagctg taagttgtct cgccagcatt gtagtctaaa aactttaaag
     1381 caaaaaaact gtctgacgtg gggagagtgt ttttgctatc agtgctttat tctttggttt
     1441 ggatttcctc ctacttggga aagttttgac tggtggcaaa aaactttaga agaaactgac
     1501 tactgcttac tgcatctgca ccttttctag actcctactt ccttcctctg taagtattag
     1561 atatggaaaa gtctataagg caaaatatca aagaaaggtt atttatgaca gattttctgt
     1621 actttcccat ctaggttgac gaggccccta tatatgggac cactaaattc aaagaatggt
     1681 ggagatcagg aggattcagc ttcgggaagg catatgaata tgggcccaat ccacacggga
     1741 ccaactcaag atccagaaag ccttcctcca atgcatccag gggagccccc agtggaagct
     1801 caccacccca cagccagagc tcttcctctg ggtatgggtc cttctcagcg tcccaggctt
     1861 cagaccccca gtccagagga cccgatatac ctcccgaaca ccatgaggaa cccacctcat
     1921 cctctggatc cagtagcaga gaggagacca ccaattcagg aagagaatcc agcacaccca
     1981 atggaaccag tgtacctaga aattcttcca gaacggatgg cacctgggag gatctcttct
     2041 gcgatgaatc actttcctcc cctgagcctc cctcgtcctc tgaggagcct gaggagcccc
     2101 cctcctcaag aagctcgccc cggcagcccc cgtcttcctc tgccgaggag gcctcgtcat
     2161 ctcagtttac agatgaggaa tacagatcct cctccttcac caccccgaag acccctcctc
     2221 cattctcaag aaagcgaaaa tttggggggt cccgaagctc tgcaagctct gctagttcag
     2281 caagttttac aagcactcca ccaaagccaa aaaagaacag agaaactcct gttcctactg
     2341 attttcctat tgatctttct gattatctta gccatgctgt atatagtaat aaaacagtaa
     2401 gttgttttgc catttatact acttctgata aagctataga gttatatgat aagattgaga
     2461 aatttaaagt tgattttaaa agcaggcatg cctgtgaatt aggatgtatt ttattgttta
     2521 taactttatc aaagcataga gtatctgcta ttaagaattt ttgctctacc ttctgcacta
     2581 taagcttttt aatttgtaaa ggagtgaata agatgcctga aatgtataat aatttatgca
     2641 agccccctta caaattactg caagagaata agccactgct aaattatgaa tttcaagaaa
     2701 aagaaaaaga ggccagctgt aattggaatt tagttgctga atttgcttgt gaatatgagc
     2761 tagacgacca ctttattatc ttagcccatt atctagactt tgcaaaacca tttccttgcc
     2821 aaaagtgtga aaacagatct cgcctcaaac ctcacaaggc tcatgaggct catcattcta
     2881 atgctaagct attttatgaa tctaaatctc agaaaaccat ttgccaacaa gccgcagaca
     2941 ctgttctagc caaaaggagg ttagagatgc tggaaatgac caggacagaa atgctatgta
     3001 agaagtttaa gaagcaccta gagagattaa gagatttaga tacaatagat ctactgtatt
     3061 atatgggtgg tgtggcctgg tactgctgct tatttgaaga gtttgaaaag aagctgcaga
     3121 aaattattca attattaaca gagaatatac ctaagtatag aaacatttgg tttaaagggc
     3181 ctattaacag tggaaaaaca agctttgctg cagccttaat agatttgcta gaagggaagg
     3241 ccttgaatat aaactgtcca tctgataagc tgccttttga actaggatgt gctttggata
     3301 aatttatggt tgtttttgag gatgtgaaag ggcaaaatag cctaaataaa gatctgcaac
     3361 cagggcaagg aataaataac cttgataact taagagatca tctagatggt gctgtagctg
     3421 taagcttaga gaagaagcat gtgaataaaa agcatcagat ttttcctcct tgtattgtta
     3481 ctgctaatga ttattttatt cccaaaacat taatagcaag atttagttat actttacact
     3541 tttccccaaa ggcaaatcta agagattccc tggatcagaa catggaaata agaaaaagaa
     3601 gaattcttca aagtggaacc actttattgc tttgtcttat ttggtgcttg cctgatacaa
     3661 cctttaagcc ttgcttacaa gaagaaatta aaaactggaa gcaaatttta cagagtgaaa
     3721 tatcatatgg taaattttgt caaatgatag aaaatgtaga agctggtcag gaccctctgc
     3781 tcaatattct tattgaggaa gagggccctg aggaaactga agaaacccaa gattctggta
     3841 ctttttctca atactcgagt ctagagggcc cgcggttcga aggtaagcct atccctaacc
     3901 ctctcctcgg tctcgattct acgcgtaccg gtcatcatca ccatcaccat tgagtttaaa
     3961 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     4021 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     4081 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     4141 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     4201 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     4261 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     4321 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     4381 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     4441 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     4501 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     4561 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     4621 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     4681 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     4741 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     4801 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     4861 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     4921 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     4981 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     5041 atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc
     5101 atagtataat acgacaaggt gaggaactaa accatggcca agcctttgtc tcaagaagaa
     5161 tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac
     5221 agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat
     5281 cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca
     5341 gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc
     5401 tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag
     5461 gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg
     5521 tgggagggct aagcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga
     5581 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     5641 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     5701 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     5761 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     5821 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     5881 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     5941 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     6001 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     6061 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     6121 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     6181 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     6241 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     6301 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6361 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6421 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     6481 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     6541 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     6601 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     6661 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     6721 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     6781 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     6841 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     6901 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     6961 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     7021 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     7081 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     7141 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     7201 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     7261 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     7321 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     7381 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     7441 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     7501 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     7561 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     7621 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     7681 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     7741 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     7801 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     7861 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     7921 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     7981 tccgcgcaca tttccccgaa aagtgccacc tgacg
//
