LOCUS       sequence-424930-        3239 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PrpsM driven expression of mNeonGreen - sequence codon-optimized for
            Salmonella.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Delgadillo-Guevara M, Halte M, Erhardt M, Popp PF
  TITLE     Fluorescent tools for the standardized work in Gram-negative 
            bacteria.
  JOURNAL   J Biol Eng. 2024 Apr 8;18(1):25. doi: 10.1186/s13036-024-00420-9.
  PUBMED    38589953
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2024-04-8"; volume: "18(1):25. doi"; pages: " 
            10.1186/s13036-024-00420-9"
COMMENT     Sequence Label: pEM8731
FEATURES             Location/Qualifiers
     source          1..3239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             483..494
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      1211..1297
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1334..1351)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1547..1566)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2050..2144)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2174..2965,2966..3034))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2797..2816
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3035..3139)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3207..3225
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgaggatc ccgggtacct gcagctagcg tcgacgaaag gctacggccg ttaattggtc
       61 gcctgagaag ttacggagag taaaaatgaa agttcgtgct tccgtcaaga aattatgccg
      121 taactgcaaa atcgttaagc gtgatggtgt catccgtgtg atttgcagtg ccgagccgaa
      181 gcataaacag cgccaaggct gattttttcg catatttttc ttgcaaagtt gggttgagct
      241 ggctagatta gccagccaat cttttgtatg tctgtacgtt tccatttgag tatcctgaaa
      301 acgggctttt cagcatggta cgtacatatt aaatagtagg agtgcatagt ggcccgtata
      361 gcaggcatta acattcctga tcagaaacac gccgtgatcg cgttaacttc gatctacggt
      421 gtcggcaaga cccgttctaa agccatcctg gctggaattc ataaggagga aaaacatatg
      481 gtatcgaagg gcgaggaaga taacatggcg tcgttgccgg cgacccacga actgcatatt
      541 tttggcagta ttaacggcgt agactttgac atggtaggtc aggggacggg taatcctaat
      601 gatggctatg aagagttaaa cttaaagtcc acgaaagggg acctgcagtt cagcccttgg
      661 attctggtcc cacacattgg ctatggcttc caccagtact tgccttatcc ggacggcatg
      721 agtcccttcc aggccgcaat ggtcgatgga tcgggctacc aggtacatcg cacgatgcaa
      781 tttgaagacg gcgcaagtct gacggtgaac tatcgttata catatgaagg tagccatatt
      841 aaaggggaag cccaagtgaa agggactgga tttccggcag atggtccggt tatgactaat
      901 tcgctgactg cggcggattg gtgtcgttcc aaaaagacgt accccaatga caaaaccatc
      961 attagcactt ttaaatggag ctacaccaca ggcaacggga agcgctaccg cagtacggcc
     1021 cgtacgacgt atacttttgc caaaccgatg gcggcgaact acctgaagaa tcaaccgatg
     1081 tatgtctttc ggaaaacaga actgaagcac agtaagaccg agttaaactt caaagaatgg
     1141 caaaaagcat ttaccgatgt aatgggcatg gatgaactgt ataaatgata agcggccgct
     1201 ctagaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     1261 ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgccctaga cctaggcgga
     1321 gatattccgc ttcctcgctc actgactcgc tccgctcggt cgttcggctg cggcgagcgg
     1381 taccggctta ctggcggggc ggaaatttcc tggaagatgc caggaagaaa cttaacaggg
     1441 aagcgataag gccacggcag agccgttttt ccataggctc cgcccccctg acaagcatca
     1501 cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggacttaaag ataccaggcg
     1561 tttccccctg gtggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc
     1621 actccgctgt tatggccgcg tttatctcat tccacgcctg acactcggtt ccgggaaggc
     1681 agttcgctcc aagctggact gtatgcacga accccccgtt cagtccgact gctgcgcctg
     1741 ttccggtaac tatcgacttg agtccaagcc ggaaagacac gacaaagcgc cactggcagc
     1801 agccactggt aagagggtgt acaagagatt actgatgcag agttcttgaa gtcagagcca
     1861 aactacggct aaactggaag gacgaatttg gtggcagcgg tcttgtacac cctgttacca
     1921 cggttcagaa gttccccaac ttactgaacc ttcgaaaaac cacctcccca ggtggttttt
     1981 tcgttttcag agcaagagat tacgacgcaa cggaaaggat ctcaagaggt tcatcttact
     2041 agtgcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc
     2101 cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgtaaa
     2161 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     2221 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     2281 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     2341 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     2401 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     2461 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     2521 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     2581 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     2641 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     2701 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     2761 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     2821 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     2881 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     2941 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     3001 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     3061 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     3121 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     3181 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcac
//
