LOCUS       sequence-424933-       10486 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAPT Exon 10 reporter containing the MS2 hairpin 30 base pairs 
            upstream of the 3′ splice site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10486)
  AUTHORS   Schmok JC, Jain M, Street LA, Tankka AT, Schafer D, Her HL, 
            Elmsaouri S, Gosztyla ML, Boyle EA, Jagannatha P, Luo EC, Kwon EJ, 
            Jovanovic M, Yeo GW
  TITLE     Large-scale evaluation of the ability of RNA-binding proteins to 
            activate exon inclusion.
  JOURNAL   Nat Biotechnol. 2024 Jan 2. doi: 10.1038/s41587-023-02014-0.
  PUBMED    38168984
REFERENCE   2  (bases 1 to 10486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Jan 2. doi: 10.1038/s41587-023-02014-0."
COMMENT     Sequence Label: lucMAPT-30U
FEATURES             Location/Qualifiers
     source          1..10486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1040..1161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1051..1070)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1105..1124
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1335..2129)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1446..1465)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2056..2075
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2196..2525)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2210..2345
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2264..2283)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     2510..2530
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(2539..2967)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2649..2670)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2861..2880
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    3013..3237
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3226..3243
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(3283..4215)
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="ASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAENA
                     VIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAWF
                     ELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDIA
                     LIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPREI
                     PLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKVK
                     GLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     misc_RNA        complement(5409..5427)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     CDS             complement(6654..8303)
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /db_xref="GI:42718124"
                     /protein_id="AAS38485.1"
                     /translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFSAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     RBS             7336..7344
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(8310..8351)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     regulatory      8357..8366
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(8438..8457)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8439..8457)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(8502..8705)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8531..8551)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8706..9085
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     9257..9276
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        9351..9455
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9456..9524,9525..10316)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9674..9693)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
     1321 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     1381 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     1441 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
     1501 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     1561 tcgccatggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac
     1621 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     1681 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     1741 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     1801 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     1861 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     1921 agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc
     1981 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     2041 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     2101 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca
     2161 gatccgaaaa tggatataca agctcccggg agctttttgc aaaagcctag gcctccaaaa
     2221 aagcctcctc actacttctg gaatagctca gaggcagagg cggcctcggc ctctgcataa
     2281 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc
     2341 gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc
     2401 atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga
     2461 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga
     2521 cacacattcc acagaattaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     2581 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     2641 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     2701 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     2761 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     2821 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     2881 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     2941 cgcttaatgc gccgctacag ggcgcgtggg gataccccct agagccccag ctggttcttt
     3001 ccgcctcaga agccatagag cccaccgcat ccccagcatg cctgctattg tcttcccaat
     3061 cctccccctt gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac
     3121 aatgcgatgc aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg
     3181 tcaaggaagg cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg
     3241 aggctgatca gcggtttaaa cttaagcttg gtaccgagct cgtcactgct cgttcttcag
     3301 cacgcgctcc acgaagctct tgatgtactt acccatttca tctggagcgt cctcctggct
     3361 gaagtggagg cccttcacct tcacgaactc ggtgttaggg aacttcttag ctccctcgac
     3421 aatagcgttg gaaaagaacc cagggtcgga ctcgatgaac atcttaggca gatcgtcgct
     3481 ggcccgaagg taggcgttgt agttgcggac aatctggacg acgtcgggct tgcctccctt
     3541 aacgagaggg atctcgcgag gccaggagag ggtaggccgt ctaacctcgc ccttctcctt
     3601 gaatggctcc aggtaggcag cgaactcctc aggctccagt ttccgcatga tcttgcttgg
     3661 gagcatggtc tcgacgaaga agttattctc aagcaccatt ttctcgccct cttcgctctt
     3721 gatcagggcg atatcctcct cgatgtcagg ccactcgtcc caggactcga tcacgtccac
     3781 gacactctca gcatggacga tggccttgat cttgtcttgg tgctcgtagg agtagtgaaa
     3841 ggccagacaa gccccccagt cgtggcccac aaagatgatt ttctttggaa ggttcagcag
     3901 ctcgaaccaa gcggtgaggt acttgtagtg atccaggagg cgatatgagc cattcccgct
     3961 cttgccggac ttacccattc cgatcagatc agggatgatg catctagcca cgggctcgat
     4021 gtgaggcacg acgtgcctcc acaggtagct ggaggcagcg ttaccatgca gaaaaatcac
     4081 ggcgttctcg gcgtgcttct cggaatcata gtagttgatg aaggagtcca gcacgttcat
     4141 ttgcttgcag cgagcccacc actgaggccc agtgatcatg cgtttgcgtt gctcggggtc
     4201 atacaccttg gaagctggtt tatgatggat gttgcctaat gagccacact tggaggtcac
     4261 cttgctcagg tcaactggtt tgtagactat ttgcacctgg agatgagaga ggaggagaga
     4321 gaagaggaaa ggcagagaga gaggaagaca gagagacaaa aaggataaaa tgggattatt
     4381 ttattaaggg tgtaactcaa tgagaagcag ctgtgtaagc ccaggccctt attggacagt
     4441 ggttcccagc tgccatgagg agcacatcct agtcagggtg gaaaaatgtg ttgtcgaaat
     4501 tctgccaacc ctgccccagg ggagctgggg actgtgggac tggagtgtct tcaatccctg
     4561 cagaacaaaa gagcctctgg gaaaaacagg gacccattgt cattgtgcaa gacccagaca
     4621 tttgctcagc aaacgcttaa gtgtaaaaat gtcactccct ttcctgacat gaaattgaca
     4681 gatcacaatc aggcatgaag aaaaaagtga tcaatatgat gagtatgcac agcatctgaa
     4741 aaatcttgct ggaaaggcag gagtggggta tctgctgggc ctcaaagagg ggaggcgtcc
     4801 gctcatcaga acaaacgggt cacctgtcca cagtggagcc actcaacgtc aacctgccca
     4861 ggtgtcgggg gaggaggcgg cagcccagtc tcaggtgcca ccaccttcag cccaacttcc
     4921 aatgggctat ggggctcctg gggacagggc tgggcatgag tggaaggaga ctttgatggg
     4981 tattctcagc ttcccaggca gggtccggag agagggtccg tcatctgccc tattctgtcc
     5041 acacagcctg cgcaggctgg gggacccaga aatgctggga caccccctcc tagaatatga
     5101 ggaaggggct tctgggaaca gtggaccgtg tgggggaatg tggggagccg ggctacattc
     5161 acccagaggt cgcagccaga tcctggggat ttattctatg cagtgtctcg caagtgtacg
     5221 cactcacacc acttcctaat aattcaagcc acagcacggc gcatgggacg tgtgaaggta
     5281 ctcacactgc cgcctcccgg gacgtgttag atattatcct ttgagccaca cttggactgg
     5341 acgttgctaa gatccagctt cttattaatt atctgcacct ttggtagcca gaaaaaagga
     5401 tgagtgacac atgggtgatc ctcatgtacg ccaccctgga cccgcctgct tgctcgcaag
     5461 gacgcctcca ctttcgatga gtgacatgcg ccccctactt gctcgcaagg atgcctccac
     5521 tttcggactt ggcagaggta gtctggggag tgacgggagg ttgagtcaca tcccagggga
     5581 gctgaaggga tagagggctg ctctgaaggc atcccagaag tgtgagtgat ttccagcggg
     5641 ctttacgaag ctgggattgc aggtcctcac ttcacaaaag gatggcatcg tgaggcctga
     5701 accccagagg ggagcgttca ccagcctgag gtcaagtgaa aaatgtgttc ggccacactg
     5761 acgagttagt aataggaagt tccccggcat ctccactcac acctggctag acccaccggc
     5821 acattcccag agaactccct gcggctgttc tcaagcctgc cgggcatcag aatcacctgt
     5881 ggatttctcc cagaagaatc agctgatcaa aacagaaccc ccaatctccc cagaatccag
     5941 aggaacccaa cctaagcaga tggcccacga gtggagatgc tgacctatgg gtggcagtgt
     6001 attctgccca gaaggcaggt ggggaaccac tgtgggtgcc ctgactatga gagcctccgg
     6061 ccgtggagac acagccctgc catctctgtc cgattaacag gggctgaagg ggcaagagaa
     6121 gagccagaac gttctctcgc ctgaggaggg cactcacagt gtagtggaga gcccaataaa
     6181 gaactgaact tccttgaaga gggtccagag gggactgggg tgttatgcgg gggtgctcag
     6241 gtgcggggag ccccacacct ccatgcacag tcccacgact ccacgctcaa ccgcgcacct
     6301 ccaggcgcag ccctaccctt ccaggcacag ccctacccct ccaggcgcag ccccccacac
     6361 ctccacgcgc agccagccac tctcaccttc ccgcctcccg gctggtgctt caggttctca
     6421 gtggagccga tcttggactt gacattcttc aggtctggca tgggcacggg ggctgtctgc
     6481 aggcggctct tggcggaaga cggcgacttg ggtggagtac ggaccactgc caccttcttg
     6541 ggctcccggg tgggtggggt tggaagggac ggggtgcggg agcggctgct gggagtgcct
     6601 ggggagccgg ggctgctgta gccgctgcga tcccctgatt ttggaggttc actcacggcg
     6661 atcttgccgc ctttcttagc cttgatcagg atctcgcgga tcttgcgggc gtccagcttg
     6721 ccggtcaggc ccttgggcac ctcgtccacg aacaccactc cgccgcgcag cttcttggcg
     6781 gttgtcacct ggctggccac atagtccacg atctccttct cggtcatggt cttgccgtgt
     6841 tccagcacga cgacggcggc aggcagctcg ccggcatcgt cgtcgggcag gccggccact
     6901 ccggcgtcga aaatgttagg gtgctgcagc aggatagact ccagctcggc tggggccacc
     6961 tggtagccct tgtacttgat cagagacttc aggcggtcca cgatgaagaa gtgctcgtcc
     7021 tcgtcccagt aggcaatgtc gccagagtgc agccagccgt ccttgtcgat cagggcgttt
     7081 gtggcctcag ggttattcac gtagccggac ataatcatag ggccgcgcac acacagctcg
     7141 ccgcgctggt tcactcccag ggtcttgccg gtgtccaggt ccaccacctt ggcctcgaag
     7201 aatggcacca ccttgcccac ggcgccaggc ttgtcgtcgc cctctggggt aatcagaatg
     7261 gcgctggttg tctcggtcag gccgtagccc tggcggatgc ctggcagatg aaagcgcttg
     7321 gccacggctt cgcccacctc cttagacagt ggggcgccgc cagaggcaat ctcgtgcagg
     7381 ttagacaggt cgtacttgtc gatcagggtg ctcttagcgg agaagctgaa cagggttggc
     7441 accagcaggg cagactgaat tttgtagtct tgcaggctgc gcaggaacag ctcctcctcg
     7501 aagcggtaca tcagcaccac ccgaaagccg caaatcaggt agcccagggt ggtgaacatg
     7561 ccgaagccgt ggtggaatgg caccacgctc agaatagcgg tgtcggggat gatctggttg
     7621 ccgaaaatag ggtcgcgggc gtgagagaag cgcacacagg cggtgcgatg aggcagggcc
     7681 acgcccttag gcaggccggt agacccagag ctgttcatga tcagggcaat ggttttgtcc
     7741 ctgtcgaaag actctggcac gaagtcgtac tcgttgaagc caggaggcag atgagatgtc
     7801 acgaatgtgt acatgctctg gaagccctgg tagtcggtct tagagtccat gatgatgatc
     7861 ttctggatga taggcagctt cttctgcacg ttcaggatct tctgcaggcc cttcttagac
     7921 acgaacacca cggtaggctg agaaatgccc atgctgttca gcagctcgcg ctcgttgtaa
     7981 atgtcgttag caggggccac ggccactccg atgaacaggg cgcccagcac tggcatgaag
     8041 aactgcagag agttctcaga gcacaccacg atgcggtggt tggtgttcag gccgtacctc
     8101 ttcatggcct cggccaggcg cacagacatc tcgaagtact cggcataggt gatgtccacc
     8161 tcaatgtggg catcggtgaa ggcaatggtg ccaggcacca gggcatacct cttcatggcc
     8221 ttgtgcagct gctcgccagc ggtgccatcc tccagagggt agaagggagc agggcccttc
     8281 ttaatgttct tagcatcggc catgctgccc gtagaatcga gaccgaggag agggttaggg
     8341 ataggcttac cgctgcccat ggtggctgca gatatccagc acagtggcgg ccgctcgagt
     8401 ctagagggcc cgtttaaacg ctagccagct tgggtctccc tatagtgagt cgtattaatt
     8461 tcgataagcc agtaagcagt gggttctcta gttagccaga gagctctgct tatatagacc
     8521 tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt
     8581 ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga
     8641 cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc
     8701 atcaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat
     8761 aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg
     8821 ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac
     8881 tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt
     8941 attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt
     9001 tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat
     9061 taataactag tcaataatca atgtcaacgc gtatatctgg cccgtacatc gcgaagcagc
     9121 gcaaaacgcc taaccctaag cagattcttc atgcaattgt cggtcaagcc ttgccttgtt
     9181 gtagcttaaa ttttgctcgc gcactactca gcgacctcca acacacaagc agggagcaga
     9241 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca taggggatcg
     9301 ggagatctcc cgatccgtcg acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     9361 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     9421 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     9481 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     9541 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     9601 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     9661 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     9721 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     9781 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     9841 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     9901 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     9961 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
    10021 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
    10081 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
    10141 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
    10201 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
    10261 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
    10321 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
    10381 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
    10441 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttc
//
